51results about How to "High interference efficiency" patented technology

The invention relates to a radar jamming method and device as well as a frequency phased array jammer. In the frequency phased array jammer, a digital radio frequency memory is used as a front-end basic component to store the received enemy detection signals, forwarding jamming or smart jamming is used flexibly, and finally, an frequency phased array antenna generates jamming signals to produce false targets more than conventional jamming ones, so that detection and tracking of an enemy radar on a moving target are effectively destroyed. The method has the innovation that the novel frequency phased array is applied, the frequency phased array antenna transits jamming signals, and the jamming signals transmitted between the array elements are different in frequency, so that on the one hand, the power of the jamming signals is strengthened in an array form, and on the other hand, the transmitted jamming signals are different in frequency and thus seriously puzzle the detection of the target of the enemy radar in frequency.

The invention discloses an RNA (Ribonucleic Acid) interference vector and application thereof. The RNA interference vector provided by the invention comprises FAD2 (fatty acid desaturase 2) gene intron and an upstream polyclone site and a downstream polyclone site on two sides of the FAD2 gene intron, wherein the FAD2 gene intron is the 60th-1190th site nucleotide from the 5' terminal of the sequence 1 in a sequence table. Proven by experiments of the invention, the invention provides an RNA interference vector suitable for plants; shRNA (short hairpin RNA) formed by expression of the RNA interference vector in a receptor is diced by Dicer into siRNA (small interfering RNA); and the siRNA can specifically inhibit the expression of a target gene, thereby achieving the aims of controlling the plant height of paddy and studying the CYP90D2/D2 gene function.

A fundus observation device which can simultaneously capture both surface images and tomographic images of the fundus oculi is provided. The fundus observation device (1) has a fundus camera unit (1A), an OCT unit (150), and an arithmetic and control unit (200). The fundus camera unit (1A) has an illuminating optical system (100) and an imaging optical system (120). The arithmetic and control unit (200) forms the surface image of fundus oculi Ef based on signals from fundus camera unit (1A). The OCT unit (150) divides low coherence light LO into the signal light LS and the reference light LR, and detects the interference light LC that can be obtained from the signal light LS passing through fundus oculi Ef and the reference light LR passing through reference mirror (174). The arithmetic and control unit (200) forms tomographic images of fundus oculi Ef based on these detecting results. A Dichroic mirror (134) combines the optical path of the signal light LS toward fundus oculi Ef into the optical path for imaging of the imaging optical system (120), and separates the optical path of the signal light LS towards fundus oculi Ef from the optical path for imaging.

The invention discloses a method for breeding non-flying Harmonia axyridis. The method uses gene interference methods to interfere with expressions of vestigial or ultrabithorax, key genes of the wingdevelopment of the Harmonia axyridis, to breed the non-flying Harmonia axyridis. The breeding of the non-flying Harmonia axyridis includes three specific methods: injecting dsRNA, feeding dsRNA/nano-carriers, or soaking the body surface of the Harmonia axyridis in dsRNA/nano-carriers/surface active agents, or spraying or dripping the dsRNA/nano-carriers/surface active agents onto the body surfaceof the Harmonia axyridis. The method breeds the non-flying Harmonia axyridis by three potential gene interference methods, and the three potential gene interference methods are highly-efficient and stable and capable of thoroughly solving a problem of difficult colonization of artificially released natural enemies Harmonia axyridis in the control area, and has important theoretical and practicalsignificance. By applying the feeding method and the body wall penetration method to gene interference, the current situation of time-consuming and labor-consuming traditional methods is completely changed, efficiency and success rates are greatly improved, and costs and operation difficulty are reduced. The method for breeding the non-flying Harmonia axyridis has obvious social and economic benefits and no potential ecological risk.

The invention relates to an energy-saving wireless local area network (WLAN) all-channel shielding method, and belongs to the technical field of wireless security. The method comprises the following steps of: starting a shielding device to scan channels of the WLAN, and recording the maximum value of a received signal strength indicator (RSSI) on each working channel; calculating the wireless propagation loss (PL) from the shielding device end to the communication equipment end with the maximum RSSI on each channel; determining the minimum transmitting power Yi,j at which the shielding deviceworking on an i channel makes the throughput of a j channel reduced to zero; finding out the maximum value of Yi,j when the shielding device works in different channels and finding out the minimum value Yk, wherein the corresponding channel k is the optimal working channel of the shielding device; and setting the working channel of the shielding device as k, and transmitting a shielding signal atYk power. By the method, all channels can be interfered at the same time, the channel throughput is reduced at the same time, the problem that an interference signal cannot be transmitted when the original channel throughput is raised and skipped due to channel skip is solved, and interference efficiency is improved.

The invention discloses a research and development method of siRNA for COVID-19 virus drug therapy, which comprises: a first part of preliminarily screening potential high-efficiency siRNA based on multiple indexes, which specifically comprises the following steps: step 1, selecting an S gene sequence as a target sequence; step 2, obtaining a corresponding siRNA double strand; step 3, screening asiRNA sequence with a concentration of 36 to 53%; step 4, screening out siRNA with free energy; step 5, defining and calculating an index I; step 6, screening out siRNA of the first 50%; step 7, defining and calculating an index II; step 8, screening out siRNA with the index II being equal to 5; step 9, defining and calculating an index III; step 10, ranking the first 50% of siRNA; step 11, directly taking all the candidate siRNAs selected in the step 10; step 12, specifically targeting a target sequence; step 13, taking the remaining siRNA as the siRNA subjected to preliminary screening; anda second part of performing interference efficiency prediction and optimization by using a machine learning model. The method has the beneficial effect of realizing safe, reliable and high-interference-efficiency siRNA design.

The invention relates to a targeting liposome containing a Claudin3 gene interference sequence. The targeting liposome takes the Claudin3 gene interference sequence as an active ingredient, and phospholipid and folic acid coupling lipid as carrier materials. The composition can be used for delivering the Claudin3 gene interference sequence to tumor cells in a targeting manner, and can give play to relatively good antitumor activity by efficiently interfering the expression of a Claudin3 gene.

The invention discloses a protodermal factor 1 (PDF1) gene for controlling plant pollen fertility, and a preparation method and application thereof. A separated protein is obtained from cabbage, cabbage type rape and turnip type rape respectively, wherein sequences of the proteins are nucleotide sequences shown by SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3 respectively. The nucleotide sequences are coding sequences (CDS) which are cloned by taking cDNA as a template. A separated polypeptide is obtained from the cabbage, the cabbage type rape and the turnip type rape respectively, wherein the sequences of the separated polypeptides are amino acid sequences shown by SEQ ID No. 4, SEQ ID No. 5 and SEQ ID No. 6 respectively. The invention discloses the application of the PDF1 gene for controlling the plant pollen fertility, and relates to isolation and cloning, expression analysis and application of the PDF1 homologous gene for controlling microspore development and anther fertility. ThePDF1 is a programmed cell death (PCD) suppression factor; and the gene has obvious expression advantage in rape anther. By weakening the expression of the PDF1 gene through gene engineering technology, the microspore can be aborted, the fertility of the pollen can be controlled, a male sterile material and a restorer material can be created, and the gene can be used for development research of androgamete and improvement on quality of farm crops.

The invention provides a method for improving RNA interfering efficiency of mandibulate insects, comprising: 1), preparing target gene specific siRNA into a solution of certain concentration using water free of RNA enzyme; 2), processing artificial feed into discs 3-5 mm in thickness and 1-2 cm in diameter, and placing the discs in a 24-pore plate; 3), adding the solution of step 1) to the discs of step 2) so that the solution is absorbed by the feed completely; 4), placing newly hatched larvae of mandibulate insects into the 24-pore plate with the feed discs of step 3), feeding for a period of time, and detecting target gene expression of the insects. By modifying the method of feeding insect siRNA, the method for improving RNA interfering efficiency of mandibulate insects is established; the method is efficient and simple and highly operable, usage of siRNA is saved, interfering effect is significant, and technical support is provided for studying the functionality of genes of mandibulate insects.

The invention discloses a vibration-sensing corrugated diaphragm, and an optical fiber acceleration sensor and a micro-vibration detection system based on the vibration-sensing corrugated diaphragm. The vibration-sensing corrugated diaphragm comprises a composite film and a plurality of annular masses, the overall shape of the composite film is a disk structure with a uniform wall thickness, the lower surface of the composite film is provided with a plurality of grooves recessed upwardly, the plurality of grooves comprise a central groove and a plurality of annular grooves, the center groove and the annular grooves have the same depth, the central groove and the annular grooves are respectively arranged concentrically with the composite film, the central groove and the plurality of annulargrooves are provided with the plurality of annular masses in one-to-one correspondence, the outer wall of the annular mass is matched with the inner wall of the corresponding groove, the thickness ofthe annular mass is smaller than the depth of the corresponding groove, the upper end surface of the annular mass abuts against the top wall of the corresponding groove, and the lower end surface ofthe annular mass is higher than the lower surface of the composite film to form a corrugated shape of the diaphragm. The vibration-sensing corrugated diaphragm has the advantages of improving the sensitivity of the micro-vibration detection and realizing the miniaturization of the optical fiber acceleration sensor.

The invention discloses a rape BnPAB5 gene and application of the rape BnPAB5 gene. A separated gene has the sequence as shown in SEQ ID No.1 and SE1 ID No.2. The PAB5 in combination with the poly(A) tail plays a special role in the transcript processes, such as starting of polyadenylation, transporting and translation, thus the expression processes of other genes can be controlled. The BnPAB5 shows preferential expression in rape anther and microspore. The expression of Arabidopsis AtPAB5 is interfered to result in abortion of the microspore. The transgenic arabidopsis of overexpression BnPAB5 is utilized as a male parent and pollinated to the transgenic arabidopsis of the interference AtPAB5, and the fertility of plant anther of filial generation and pollen can be recovered. As the complementary test shown, the rape BnPAB5 and the arabidopsis AtPAB5 have the same functions, such as the functions of controlling the development of the rape anther and arabidopsis anther and the fertility of the pollen; the expression level of the PAB5 can be controlled by genetic engineering technology, thus the fertility of the pollen can be controlled, and male sterility line materials and restoring line materials can be created; and the rape BnPAB5 gene can be applied to the study on male gametes development and the improvement of quality of crop.

The invention relates to the field of biotechnology and particularly discloses a method for researching helicoverpa armigera larva RNA interference efficiency. The method for researching the helicoverpa armigera larva RNA interference efficiency is established by improving a method for feeding helicoverpa armigera larva siRNA (small interfering RNA), the lepidopteron helicoverpa armigera larva RNA interference efficiency is improved, and experimental results are stabilized. The method provided by the invention is efficient and simple, and is high in operability, the dosage of siRNA is definite, the repeatability is high, and technical supports for researching functions of genes of lepidopteron helicoverpa armigera are provided.

The invention provides a double optical path detection lateral shearing interferometer, which comprises a beam-splitting cubic prism 1, a beam-splitting cubic prism 2, a right-angle reflecting prism 1 and a right-angle reflecting prism 2, wherein an incident optical axis is vertical to an incident surface of the beam-splitting cubic prism 1 and is positioned at the center of the incident surface of the beam-splitting cubic prism 1; beam-splitting surfaces of the beam-splitting cubic prism 1 and the beam-splitting cubic prism 2 are positioned on the same plane, and the beam-splitting cubic prism 2 is positioned between an emergent surface of transmitted light rays of the beam-splitting cubic prism 1 and a hypotenuse surface of the right-angle reflecting prism 1; the hypotenuse surface of the right-angle reflecting prism 1 is parallel to the incident surface of the beam-splitting cubic prism 1; the right-angle reflecting prism 2 is positioned on the upper side of the beam-splitting cubic prism 2, and a hypotenuse surface of the right-angle reflecting prism 2 is parallel to the emergent surface of reflected light rays of the beam-splitting cubic prism 1. The invention further provides a lateral shearing interference method, which has higher interference efficiency, improves performance of the interferometer, and reduces the strict requirement of the interferometer on performance of a beam splitting film.

The invention discloses a method for achieving the radar noise interference based on multi-order inverse Fourier convolution transform, and the method is characterized in that firstly, the method canachieve the covering of a target echo and also can resist side-lobe blanking for a high-density false target; secondly, it must be a pulse signal for resisting sidelobe cancellation; thirdly, the interference has the in-pulse modulation features the same as a target echo. The method gives consideration to the technical characteristics of noise interference and deception jamming, and the interference waveform generated by the method is better in coherence. The method enables an interference pulse signal to pass a detection processing system of a radar easily, also makes the most of the energy of an interference machine, and can achieve the purpose of effective interference.

The invention discloses an RNA interference method based on a bemisia tabaci nymph body wall permeation approach. The method comprises the following steps of (1) cultivation of cotton seedlings; (2) insect inoculating and spawning; (3) RNA interference based on the body wall permeation approach, wherein dsRNA and an exogenous control gene dsEGFP are prepared, the dsRNA is uniformly mixed with a nano material of a star-shaped polymer according to the volume ratio of 1:1, the mixture is carefully dropped on nymphs, cotton leaves are inserted back into a bottle filled with clean water after the liquid drops are absorbed by the insects, the insects are placed into a culture box for continuing feeding, the death number is recorded 2 days later, and nymphs samples are collected. According to themethod, by utilizing the strong permeability of the novel nano material, the dsRNA quickly permeates into bemisia tabaci bodies through the body walls, so that the death rate is obviously reduced, the interference efficiency is improved, and the timeliness and the repeatability are high. In addition, the use amount of the dsRNA in the method is very small and reaches about 0.3 microliter per individual, the interference efficiency is ensured, and meanwhile the experimental cost is greatly reduced; the method has a good application prospect.

The invention discloses an intelligent electromagnetic information protection system. The system comprises a main control module, a state detection module, a network communication module, a broadbandinterference information source generation module, a targeted comb-shaped information source generation module, a VGA interface module, a DVI interface module, an HDMI interface module, a power supplymodule, an output efficiency detection module, a power output control module, a multi-channel broadband power amplifier and an antenna. The power supply module, the network communication module, thestate detection module, the output efficiency detection module, the power output control module, the broadband interference information source generation module and the targeted comb-shaped information source generation module are all connected with the main control module. According to the system, interface electromagnetic information such as VGA, DVI, HDMI and the like can be automatically and respectively selected to be effectively protected, the interference modes of VGA, DVI and HDMI can be automatically and simultaneously started at high power, and effective electromagnetic protection can be implemented on important secret-related places, large-scale comprehensive display platforms and high-density interconnection interfaces.

The invention discloses a rule weight distribution siRNA design method based on grid search, and relates to the technical field of bioinformatics. The method comprises the following steps: S1, training siRNA data to obtain siRNA design rule weights; and S2, based on the obtained siRNA design rule weights, screening out siRNA with high silence efficiency from the candidate siRNA set. According to the method, each rule is differentially treated according to different weights, and based on the multi-rule design of different weights, all rules can be prevented from being treated according to the same importance degree, important rules in the rules can be highlighted, effective siRNA and ineffective siRNA can be distinguished, and the efficiency of candidate siRNA sequences can be quantitatively predicted.

The invention belongs to the field of biological genes. Chronic pain is one of forms of expression for neuronal plasticity change, and has the physiological characteristics of increased reactivity of pain sense, treatment difficulty, unclear mechanism, and more side effects by the use of opiate receptor antagonist in treatment. Researches prove that the NMDA regulation is related to the chronic pain. Therefore, the invention provides a small RNA for a human and rat-homologous NMDA receptor NR2B, which has a sequence shown as SEQ ID NO:1. The invention also provides application of the small RNA to medicaments for treating the chronic pain. The small RNA can be directly injected, can inhibit the expression of the NMDA receptor NR2B for human and a rat in vitro and in vivo to achieve the effect of treating the chronic pain, and has the advantages of convenient administration route, no addiction, tolerance and other reactions, and long-term use.