51results about How to "High interference efficiency" patented technology

The invention discloses an RNA (Ribonucleic Acid) interference vector and application thereof. The RNA interference vector provided by the invention comprises FAD2 (fatty acid desaturase 2) gene intron and an upstream polyclone site and a downstream polyclone site on two sides of the FAD2 gene intron, wherein the FAD2 gene intron is the 60th-1190th site nucleotide from the 5' terminal of the sequence 1 in a sequence table. Proven by experiments of the invention, the invention provides an RNA interference vector suitable for plants; shRNA (short hairpin RNA) formed by expression of the RNA interference vector in a receptor is diced by Dicer into siRNA (small interfering RNA); and the siRNA can specifically inhibit the expression of a target gene, thereby achieving the aims of controlling the plant height of paddy and studying the CYP90D2 / D2 gene function.

The invention discloses a SiRNA (small interfering ribose nucleic acid) for inhibiting homosapiens chromosome 2 open reading frame 3(C2 or f3) (GCF) gene expression, which belongs to the field of medical genetic engineering. Deoxyribose nucleic acid (DNA) for an encoding SiRNA is selected from arbitrary group of (a), (b) and (c): (a) a base sequence of a positive-sense strand is shown as SEQ ID No.1, and the base sequence of an antisense strand is shown as SEQ ID No.2; (b) the base sequence of the positive-sense strand is shown as SEQ ID No.3, and the base sequence of the antisense strand is shown as SEQ ID No.4; and (c) the base sequence of the positive-sense strand is shown as SEQ ID No.5, and the base sequence of the antisense strand is shown as SEQ ID No.6. The invention further discloses a recombinant plasmid which comprises the DNA of the encoding SiRNA and a GCF-shRNA-HeLaCGMCC (China general microbiological culture collection center) No.5821, and a cell line has high RNA interfering efficiency, strong specificity and no toxic and side effects and can effectively inhibit GCF gene expression. The SiRNA for inhibiting GCF gene expression, the recombinant plasmid and the GCF-shRNA-HeLa monoclonal cell can also be used for preparing medicines for treating cervical cancer.

The invention discloses a vibration-sensing corrugated diaphragm, and an optical fiber acceleration sensor and a micro-vibration detection system based on the vibration-sensing corrugated diaphragm. The vibration-sensing corrugated diaphragm comprises a composite film and a plurality of annular masses, the overall shape of the composite film is a disk structure with a uniform wall thickness, the lower surface of the composite film is provided with a plurality of grooves recessed upwardly, the plurality of grooves comprise a central groove and a plurality of annular grooves, the center groove and the annular grooves have the same depth, the central groove and the annular grooves are respectively arranged concentrically with the composite film, the central groove and the plurality of annulargrooves are provided with the plurality of annular masses in one-to-one correspondence, the outer wall of the annular mass is matched with the inner wall of the corresponding groove, the thickness ofthe annular mass is smaller than the depth of the corresponding groove, the upper end surface of the annular mass abuts against the top wall of the corresponding groove, and the lower end surface ofthe annular mass is higher than the lower surface of the composite film to form a corrugated shape of the diaphragm. The vibration-sensing corrugated diaphragm has the advantages of improving the sensitivity of the micro-vibration detection and realizing the miniaturization of the optical fiber acceleration sensor.