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Rule weight distribution siRNA design method based on grid search

A design method and weight distribution technology, applied in the field of bioinformatics, can solve problems such as not considering different treatment

Pending Publication Date: 2021-06-11
深圳市新合生物医疗科技有限公司
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  • Claims
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Problems solved by technology

However, the siRNA design based solely on existing rules has a strong preference on the one hand, and is usually only applicable to certain specific data; on the other hand, the method based on existing rules regards the weight of each rule as the same , without taking into account that each rule is treated differently with a different weight

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  • Rule weight distribution siRNA design method based on grid search
  • Rule weight distribution siRNA design method based on grid search
  • Rule weight distribution siRNA design method based on grid search

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Embodiment Construction

[0057] Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0058] One embodiment of the present invention: a method for designing siRNA based on grid search for rule weight distribution, comprising the following steps:

[0059] S1, training from siRNA data to obtain the weight of siRNA design rules;

[0060] S2. Screening siRNAs with high silencing efficiency from the candidate siRNA set based on the obtained siRNA design rule weights.

[0061] The grid search-based rule weight distribution ...

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Abstract

The invention discloses a rule weight distribution siRNA design method based on grid search, and relates to the technical field of bioinformatics. The method comprises the following steps: S1, training siRNA data to obtain siRNA design rule weights; and S2, based on the obtained siRNA design rule weights, screening out siRNA with high silence efficiency from the candidate siRNA set. According to the method, each rule is differentially treated according to different weights, and based on the multi-rule design of different weights, all rules can be prevented from being treated according to the same importance degree, important rules in the rules can be highlighted, effective siRNA and ineffective siRNA can be distinguished, and the efficiency of candidate siRNA sequences can be quantitatively predicted.

Description

technical field [0001] The present invention relates to the technical field of bioinformatics, in particular to a grid search-based method for designing siRNA with regular weight distribution. Background technique [0002] RNA interference (RNA interference, RNAi) is a post-transcriptional gene silencing phenomenon induced by short double-stranded RNA (Small interfering RNA, siRNA). Functional exploration, gene expression regulation mechanism and other fields have accelerated the research of functional genomics, and also promoted the research of gene therapy and other related fields. [0003] siRNA is a double-stranded RNA composed of 20 to 27 base pairs. Its efficiency is affected by many factors such as the target gene mRNA sequence and its own sequence. One of the key factors is the siRNA sequence design. A large number of studies have proved that siRNA designed for the same target mRNA The effects vary greatly. How to screen siRNAs for efficient silencing of target gen...

Claims

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Application Information

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IPC IPC(8): G16B20/00G06K9/62
CPCG16B20/00G06F18/214G06F18/241
Inventor 万季刘鹏沈一鸣徐韵婉潘有东王弈宋麒
Owner 深圳市新合生物医疗科技有限公司
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