The invention discloses a method for
RNA interference of
whitefly eggs, comprising the following steps: dsRNA, star-shaped
polymer nanomaterials and surfactants are mixed to obtain an interference
reagent; clean cotton seedlings are placed in a
greenhouse insect cage to receive eggs for 12h-48h, cotton seedlings are taken out and the number of eggs on the cotton seedlings is recorded; the interference
reagent is drawn with a
pipette and dripped on the egg body, the dripping process keeps the
pipette from touching the egg body, and the droplet is absorbed by the egg body, and the cotton seedlings with eggs are placed back in the
greenhouse insect cage to continue to be raised; after the preset time of raising, samples of some eggs are taken,
RNA is extracted, and
RNA interference efficiency is identified, and the remaining eggs are normally raised in the
greenhouse insect cage until the adult emerges, the number of emerging adults is recorded, and the phenotypic changes of adults are observed. The
RNA interference method of
whitefly eggs of the present invention can eliminate the damage of
microinjection to eggs, and while ensuring the
hatching rate of eggs, interference with target genes is achieved, breaking through the
bottleneck of the interference technology of
whitefly eggs.