The present invention discloses a
nucleic acid amplification product detection
reagent and a detection method, and the detection
reagent is prepared by the following method: 1) using
cellobiose and 1,3-propanedithiol as carbon sources, and preparing
carbon quantum dots by a hydrothermal method; 2) modifying
copper ions on the surface of
carbon quantum dots to obtain the detection
reagent; when there is a byproduct
pyrophosphate generated by the
nucleic acid amplification reaction, the
fluorescence of the
carbon quantum dots will be enhanced, so that the solution obtained after the reaction of the detection reagent with the product of
nucleic acid amplification is subjected to
fluorescence detection, and the qualitative and / or quantitative detection of the nucleic acid amplification product is realized. The present invention provides a nucleic acid amplification product detection reagent and a method, and a reagent that can be used for the detection of
pyrophosphate, a byproduct of nucleic acid amplification, is prepared by using
copper ions to modify carbon
quantum dots, and is successfully applied to the detection of nucleic acid amplification products, and can realize the qualitative and quantitative detection of nucleic acid amplification products, and it has the advantages of being simple and convenient, fast and intuitive, and highly prepared.