The invention discloses an
enzyme immunoassay method for progestational
hormone in excrement of sows. The method comprises the steps of: (1)
sample collection: collecting the excrement of the sows, weighing and dissolving 0.5 g of excrement of the sows into 20 ml of measuring buffer liquid, carrying out centrifuging, taking and placing supernatant at 4 DEG C to be detected; and (2) measuring content of progestational
hormone in a sample by utilizing competitive ELISA (
enzyme-linked immuno
sorbent assay): measuring according to an ELISA process. According to the
enzyme immunoassay method for the progestational
hormone in the excrement of the sows, the detection sensitivity of progesterone reaches 0.2 ng / ml or 8 ng / g; the
recovery rate of the progesterone is 95.78%; inter-
assay and intra-
assay variable coefficients are respectively 6.8% (n is equal to 9) and 11.23% (n is equal to 8); the specificity of the progesterone is that the cross reaction rates of the progesterone to estradiol,
estriol and oestrone are all less than 0.01%, and the cross
reaction rate of the progesterone to
testosterone is 0.35%; the stability is good; the required sample is easy to collect, animals are not injured, a measured result is reliable, and a method for measuring blood of the animals can be replaced to monitor a series of reproductive activities of the animals.