95 results about "Dicer" patented technology

The invention provides compositions and methods for signal activated RNA interference (saRNAi), preferably in vivo. The invention provides polynucleotides that switches between an inactive form and an active form upon covalent or non-covalent interaction with one or more specific chemical signals, such as disease-specific mRNA, miRNA, or other cellular RNA products with sequences that characterize diseased states of the cell. The interaction between the subject polynucleotides and the signals is preferably mediated by hybridization, which exposes, facilitates the formation, and / or allows the formation of a substrate that can be processed by proteins of the RNAi pathway (such as Dicer). The input and output of multiple different polynucleotides of the invention can form an in vivo signaling network. In addition, the multiple input signals can be integrated to modulate the activity of the subject polynucleotides.

The present invention provides novel, stable nucleic acid-lipid particles comprising one or more Dicer-substrate dsRNAs and / or small hairpin RNAs (shRNAs), methods of making the particles, and methods of delivering and / or administering the particles (e.g., for the treatment of a disease or disorder). In some embodiments, the nucleic acid-lipid particles of the invention comprise Dicer-substrate dsRNAs and / or shRNAs. In other embodiments, the nucleic acid-lipid particles of the invention comprise Dicer-substrate dsRNAs and / or shRNAs in combination with one or more additional interfering RNAs (e.g., siRNA, aiRNA, and / or miRNA). In further embodiments, the Dicer-substrate dsRNAs and / or shRNAs present in the nucleic acid-lipid particles of the invention are chemically synthesized.

The invention provides a bean curd dicer. The dicer comprises a base, a horizontal guide rail, a tray, a first cutting device and a second cutting device, wherein the horizontal guide rail is fixed on the base; the tray is used for arranging bean curd, supported on the horizontal guide rail and capable of sliding along the horizontal guide rail; the first cutting device is fixed on the base and located on one side of the tray, the tray passes through the first cutting device in a sliding mode, and the bean curd on the tray is cut by the first cutting device horizontally; and the second cutting device is positioned above the horizontal guide rail and capable of sliding vertically, and the bean curd on the tray is cut by the second cutting device vertically. According to the bean curd dicer, the structure is simple, the action process can be performed easily, the whole size of the dicer is small, and environmental conditions in bean curd dicing can be controlled easily.

The present invention provides methods for attenuating gene expression in a cell using gene-targeted double stranded RNA (dsRNA). The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene).

This invention provides a method for the in vivo delivery of oligonucleotides. The invention utilizes the presence of one or plurality of HES linked to an oligonucleotide to deliver a nucleic acid sequence of interest into the cytoplasm of cells and tissues of live organisms. The delivery vehicle is nontoxic to cells and organisms. Since delivery is sequence-independent and crosses membranes in a receptor-independent manner, the delivered oligonucleotide can target complementary sequences in the cytoplasm as well as in the nucleus of live cells. Sequences of bacterial or viral origin can also be targeted. The method can be used for delivery of genes coding for expression of specific proteins, antisense oligonucleotides, siRNAs, shRNAs, Dicer substrates, miRNAs, anti-miRNAs or any nucleic acid sequence in a living organism. The latter include mammals, plants, and microorganisms such as bacteria, protozoa, and viruses.

A dicing tool having: a foodstuff holder for holding a block of foodstuff and a cutting base; the cutting base having: a rectangular framework for sliding the foodstuff holder therealong; a first part having an triangular shaped front edge, equally spaced triangular cutting blades being arranged in a triangular arrangement that follows the profile of the triangularly shaped front edge; a handle; a second part having a second upper surface and a right front edge and a left front edge arranged in a deep V shape, with continuous blades attached to the right and left front edges; wherein the second upper surface is always parallel to the first surface and can be slidingly reciprocated between an upper position and a lower position where the horizontal blade is substantially on a level with the first surface and the base of the triangular cutting blades.

The invention discloses an RNA (Ribonucleic Acid) interference vector and application thereof. The RNA interference vector provided by the invention comprises FAD2 (fatty acid desaturase 2) gene intron and an upstream polyclone site and a downstream polyclone site on two sides of the FAD2 gene intron, wherein the FAD2 gene intron is the 60th-1190th site nucleotide from the 5' terminal of the sequence 1 in a sequence table. Proven by experiments of the invention, the invention provides an RNA interference vector suitable for plants; shRNA (short hairpin RNA) formed by expression of the RNA interference vector in a receptor is diced by Dicer into siRNA (small interfering RNA); and the siRNA can specifically inhibit the expression of a target gene, thereby achieving the aims of controlling the plant height of paddy and studying the CYP90D2 / D2 gene function.

Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Ago2 because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules.

The present invention provides a small-activating RNA, which comprises a sense sequence containing 25-30 nucleotides and an antisense sequence containing 25-30 nucleotides, wherein at least 80% of the antisense sequence is complementary to the sense sequence, the sense sequence or antisense sequence contains the matching fragment having 19-25 nucleotides, and at least 80% of the sequence of the matching fragment is complementary to the fragment of the target gene regulation sequence. According to the present invention, the small-activating RNA adopted as the Dicer substrate can activate the gene expression at the transcriptional and epigenetic levels, effectively imitates the natural maturation process of the endogenous double-stranded small RNA, and effectively increases the efficiency of RNA activation.

A manual food dicer includes a frame equipped with a cross blade and two guiding rails, a carriage movable in translation, and a longitudinal cutting knife equipped with triangular blades, wherein the knife cooperates with positioning mechanism enabling it to be elastically mounted between a working position in which the triangular blades jut out over the upper surface of the frame, and wherein a retracted position in which the triangular blades are retracted, the translation of the carriage causing the knife to move into retracted position.

MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. The miRNAs are useful to diagnose and treat cancers.

Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Ago2 because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules.

Abstract of the DisclosureABSTRACT Dicer (e.g., DCR-1) interactors are disclosed as are methods to positively or negatively modulate Dicer activity. Uses of Dicer interactors as drug targets are featured. Also featured are uses of Dicer interactors and modulators of same to modulate various Dicer functions in vitro, in cell cultures, and in vivo.

The present invention relates, in general, to RNA silencing and, in particular, to a method of effecting targeted delivery of an RNA silencing moiety using a targeting moiety that binds to a cell surface receptor and mediates internalization of the RNA silencing moiety to be accessible to Dicer. Also provided is a chimeric nucleic acid molecule comprised of a targeting moiety and an RNA silencing moiety, wherein the targeting moiety is an aptamer and the RNA silencing moiety comprises a Dicer substrate.

A manually operated food dicer having a frame structure which is supported above a support surface, the frame structure having a top surface that includes at least a first slicing blade and at least one carriage guide configuration. A food item carriage displaceably deployed on the frame structure by way of the carriage guide configuration, the food item carriage including an upper receptacle for receiving food items and a lower dicing blade platform. As the food item carriage traverses the length of the frame structure in a cutting direction, the upper receptacle passes over the slicing blade resulting in a slide of the food item being deposited on the lower dicing blade platform, after which the carriage guide configuration brings a top surface of the lower dicing blade platform into contact with a bottom dicing surface of the frame structure further cutting the food item slice into diced food cubes.

The invention relates to an article for daily use, in particular to a simple dicer, which comprises a fixed frame. The simple dicer is characterized in that transverse blades are uniformly arranged in the fixed frame; guide rails are arranged at the bottom of the fixed frame; a sliding frame capable of sliding is arranged on the guide rails; vertical blades are uniformly arranged in the sliding frame; a storage plate is arranged between the guide rails; handles are arranged on the two sides of the sliding frame. The simple dicer has the beneficial effects that the simple dicer is simple in structure and convenient to operate; food materials can be easily diced, so that convenience in life is improved.

The invention provides a bean curd dicer. The dicer comprises a base, a horizontal guide rail, a tray, a first cutting device and a second cutting device, wherein the horizontal guide rail is fixed on the base; the tray is used for arranging bean curd, supported on the horizontal guide rail and capable of sliding along the horizontal guide rail; the first cutting device is fixed on the base and located on one side of the tray, the tray passes through the first cutting device in a sliding mode, and the bean curd on the tray is cut by the first cutting device horizontally; and the second cutting device is positioned above the horizontal guide rail and capable of sliding vertically, and the bean curd on the tray is cut by the second cutting device vertically. According to the bean curd dicer, the structure is simple, the action process can be performed easily, the whole size of the dicer is small, and environmental conditions in bean curd dicing can be controlled easily.

The present invention provides double-stranded RNA molecules that mediate RNA interference in target cells, preferably hepatic cells. The invention also provides double-stranded RNA molecules that are modified to be resistant to nuclease degradation, which inactivates a virus, and more specifically, hepatitis C virus (HCV). The invention also provides a method of using these modified RNA molecules to inactivate virus in mammalian cells and a method of making modified small interfering RNAs (siRNAs) using human Dicer.

The invention provides a method for cultivating dwarf rice by utilizing Dicer protein OsDCL3b in rice. In the method, the expression of an OsDCL3b gene in a rice cell is inhibited by utilizing a transgene, specially, the expression of the OsDCL3b gene in the rice is inhibited by utilizing a RNA (ribonucleic acid) interference technology so as to obtain the dwarf rice. The method has important significance in lodging resistance of rice and has wide application prospect.

Provided are a piezoelectric vibrator and a high efficient method manufacturing the same. The piezoelectric vibrator (70, 72) includes a tuning-fork vibrator (78) having a base (74) and legs (76a, 76b). The piezoelectric vibrator (70, 72) is formed as a laminate of two piezoelectric substrates (80, 82), an intermediate electrode (84), surface electrodes (86, 88, 90), and a whole surface electrode (94). The surface electrodes (86, 88, 90) are divided by division portions (92a, 92b) extending from the base (74) to the legs (76a, 76b). The division portions (92a, 92b) are formed wider at portions joining to a circuit board than at the other portions. The division portions (92a, 92b) are formed by dividing an electrode formed on the whole surface of a piezoelectric plate constituting the vibrator (78) by means of a dicer, or by dividing the electrode by means of laser irradiation or etching. The tuning-fork vibrator exhibits good insulation reliability at the time of mounting on a circuit board, and has a high vibrator sensitivity and a high S / N ratio.

The invention relates to a method for cultivating cotton resisting verticillium dahliae. The method comprises the step that genes of a sequence shown in the SEQ ID NO.1 and genes of the inverted repeat sequence are guided into a cotton genome. According to the method, verticillium dahliae virulence genes are guided into a cotton plant, so that the genes are transcribed in the cotton plant to generate a double-stranded RNA structure corresponding to the verticillium dahliae virulence genes, Dicer-like protein in the cotton plant cuts the double-stranded RNA structure into siRNA with the size being 20-25nt, and the siRNA can recognize the virulence genes mRNA complementary with the sequence in verticillium dahlia infecting the cotton, degrades the virulence genes and lowers the pathogenicity of verticillium dahlia.

The invention discloses an RNA (Ribonucleic Acid) interference vector and application thereof. The RNA interference vector provided by the invention comprises FAD2 (fatty acid desaturase 2) gene intron and an upstream polyclone site and a downstream polyclone site on two sides of the FAD2 gene intron, wherein the FAD2 gene intron is the 60th-1190th site nucleotide from the 5' terminal of the sequence 1 in a sequence table. Proven by experiments of the invention, the invention provides an RNA interference vector suitable for plants; shRNA (short hairpin RNA) formed by expression of the RNA interference vector in a receptor is diced by Dicer into siRNA (small interfering RNA); and the siRNA can specifically inhibit the expression of a target gene, thereby achieving the aims of controlling the plant height of paddy and studying the CYP90D2 / D2 gene function.

A dicer includes a body providing a motor, a container and a container cover, a feeding tube extending on the container cover, a push rod in the feeding tube, a power output shaft in the container, deceleration components and cutter disks on the output shaft. The cutter disks comprises a slicing cutter disk and a dicing cutter disk, a block in the container cover, a handle in the slicing cutter disk, and through-holes in the dicing cutter disk. The dicer is able to cut food into pieces with the same shape and size. The deceleration components are provided in the container and have simple structure, which occupy small space. The handle is provided on the slicing cutter disk for facilitating to detach the slice cutter disk for cleaning, and a through-hole is defined in the dicing cutter disk for facilitating to detach the dicing cutter disk for cleaning.

A dicer is provided with a power output shaft in the container, and deceleration components and cutter disks on the output shaft. The cutter disks comprises a slicing cutter disk and a dicing cutter disk, a block in the container cover, a handle in the slicing cutter disk, and through-holes in the dicing cutter disk. The dicer is able to cut food into pieces with the same shape and size, and the dicing effectiveness is substantially perfect. The deceleration components have simple structure, which occupy small space and are convenient for household use. The handle is provided on the slicing cutter disk to facilitate detachment of the slice cutter disk for cleaning, and a through-hole is defined in the dicing cutter disk to facilitate detachment of the cutter disk for cleaning. The block makes the slicing cutter disk stable when rotating, to facilitate dicing.

The invention relates to a watermelon pulp-taking dicer. The watermelon pulp-taking dicer is characterized by comprising an executing system and a power system, wherein an executing mechanism comprises a fixing base, a pulp-taking device and a dicing device. The watermelon pulp-taking dicer has the advantages of being high in automation degree, convenient to operate, clean and sanitary, economical and practical and less in waste. Diced watermelon peel and watermelon blocks are in regular block shapes, and the follow-up processing is facilitated.

The invention discloses a meat dicer that cuts meat into pieces, and the meat dicer comprises a meat feeding part; a first cutting part that comprises a first central shaft that rotates vertical to the meat transfer direction, and multiple first cutting blades that extend from the first central shaft and rotate through the first central shaft so as to cut the meat from the meat feeding part vertical to the meat transfer direction and form thread-shaped meat; and a first cutting part that comprises a second central shaft that rotates vertical to the first central shaft and multiple first cutting blades that are arranged at the periphery of the second central shaft and are isolated from each other in a preset isolation distance and rotate through the second central shaft so as to cut the thread-shaped meat from the first cutting part vertical to the cutting direction of the first cutting part to form sliced meat.

The invention provides a high-speed dicer which greatly improves transverse cutting efficiency. The high-speed dicer comprises a frame, an electric cabinet and a feeding platform, wherein the electric cabinet is fixed on the frame; the feeding platform is provided with a feeding conveying belt; the frame is further provided with an upper clamping conveying belt and a pressing cylinder; the pressing cylinder is connected with the upper clamping conveying belt; the upper clamping conveying belt is pressed to the feeding conveying belt; a longitudinal slitting device is arranged at the downstream of the feeding conveying belt; a transverse dicing device is arranged at the downstream of the longitudinal slitting device; a lower cutter is arranged below the transverse dicing device; a diced meat discharging belt is arranged below the lower cutter; the longitudinal slitting device comprises a longitudinal cutting device mounting plate; a longitudinal pre-cutting knife and a longitudinal cutter are arranged on the longitudinal cutting device mounting plate in parallel; and a stopping plate is arranged at the downstream of the longitudinal cutter and is mounted on the longitudinal cutting device mounting plate through a spring.