Establishment of novel RNA interference method for bemisia tabaci nymphs

Abstract

The invention discloses an RNA interference method based on a bemisia tabaci nymph body wall permeation approach. The method comprises the following steps of (1) cultivation of cotton seedlings; (2) insect inoculating and spawning; (3) RNA interference based on the body wall permeation approach, wherein dsRNA and an exogenous control gene dsEGFP are prepared, the dsRNA is uniformly mixed with a nano material of a star-shaped polymer according to the volume ratio of 1:1, the mixture is carefully dropped on nymphs, cotton leaves are inserted back into a bottle filled with clean water after the liquid drops are absorbed by the insects, the insects are placed into a culture box for continuing feeding, the death number is recorded 2 days later, and nymphs samples are collected. According to themethod, by utilizing the strong permeability of the novel nano material, the dsRNA quickly permeates into bemisia tabaci bodies through the body walls, so that the death rate is obviously reduced, the interference efficiency is improved, and the timeliness and the repeatability are high. In addition, the use amount of the dsRNA in the method is very small and reaches about 0.3 microliter per individual, the interference efficiency is ensured, and meanwhile the experimental cost is greatly reduced; the method has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biology and relates to the construction of a novel RNA interference method for whitefly nymphs. Background technique [0002] Bemisia tabaci belongs to the order Hemiptera and belongs to the family Aleyrodidae. As a worldwide agricultural pest, it is widely distributed in China, Japan, Malaysia, India, the United States and other countries. Bemisia tabaci is a rapidly evolving compound species. Bemisia tabaci was first introduced into my country in 1949, and broke out in the 1990s, causing huge economic losses to my country's agricultural production. Q bemisia tabaci was first discovered in Yunnan Province of my country in 2003, and now Q bemisia tabaci has replaced B bemisia tabaci as the main endangered species in my country. Bemisia tabaci is a polyphagous pest with a wide host spectrum. Its host plants involve 74 families and 420 species, mainly including Brassicaceae, Cucurbitaceae, Fabaceae, Solanaceae, etc....

AI Technical Summary

Problems solved by technology

[0005] At present, the operation technology of RNAi on Bemisia tabaci adults has been relatively mature, but the mature experience of RNAi on Bemisia tabaci nymphs at home and abroad is too little. In this experiment, new nanomaterials were used, and the 3-4 instar nymphs of Bemisia tabaci were used as samples. Experimental object, successfully established a set of mature RNAi technology

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