66results about How to "Experimental results are stable" patented technology

The invention discloses an SAR back projection imaging method based on feature distance subspace. The method is on the basis of distance history vector, and the concept of distance history vector match degree is put forward from the angle that whether a conventional BP algorithm can focus; and then, based on minimum mean square error criterion and principal component analysis principle, the concept of feature distance subspace is put forward in the SAR imaging field. The measured distance history vector is optimally corrected in the feature distance subspace, so that measurement noise is suppressed very well, the distance history vector match degree is improved, compensated phase position is subjected to accurate estimation, and finally, good focusing effect is obtained. The SAR back projection imaging method is suitable for SAR two-dimension imaging and three-dimensional imaging of any system, and thus the method has very good popularization performance. Finally, since correcting the distance history vector in the feature distance subspace is a linear operation, the method has very high operability.

The invention discloses a method for testing biotoxicity of oil-field wastewater by employing vibrio qinghaiensis Q67. The oil-field wastewater is taken as test wastewater, the vibrio qinghaiensis Q67 is taken as a bioindicator and a 96-pore microwell plate is taken as a carrier; by adopting a 96-pore microwell plate analyzing method, the luminous intensity is detected by adopting a microwell plate spectraphotometer; and the luminescent inhibition rate is calculated by using the luminous intensity of an experiment group and a control group and Zn(NO3)2 is set as a toxic reference substance, thereby judging the biotoxicity of the oil-field wastewater. According to the method, the toxicity of the oil-field wastewater is detected and analyzed by using a microplate method; and the comprehensive toxicity of the oil-field wastewater is judged through calculating the luminescent inhibition rate of luminescent bacteria; the problem that conventional physicochemical indexes cannot represent the biotoxicity of the oil-field wastewater is solved; the method is fast, sensitive and convenient to popularize and apply; the selected toxic reference substance Zn(NO3)2 is steady in experiment result, low in price and medium in toxicity.

The invention provides a method for screening and identifying potassium-rich tobacco. The method comprises steps as follows: 1) tobacco seeds are sown on a low-potassium culture medium for seedling culture, tobacco seedlings are graded according to the reaction degree of tobacco seedlings to low potassium, tobacco plants which have high yellowing degree and are active are screened and transplanted to a full nutrient solution for growth; 2) when the tobacco seedlings grow to have 6-8 leaves, the tobacco seedlings are transplanted to a field, tobacco leaves in the same leaf positions of the selected plants are subjected to fixation, drying, grinding and sieving in the manure period, the content of potassium is measured, and the plant with the highest potassium content is labeled; 3) seeds of the plant with the highest potassium content are harvested, planting and screening in step 1) and step 2) are performed repeatedly until genotype homogeneity is realized, and the variety of potassium-rich tobaccos is bred. Based on the principle that potassium-rich variants are not resistant to low potassium, potassium-rich tobacco variant materials are screened by use of the low-potassium culture medium, field verification is combined in the later period, and the result is accurate and stable.

The invention discloses a method for identifying breeds of Chinese cabbage and a special kit thereof. The kit comprises a primer combination I, a primer combination II, a primer combination III, a primer combination IV, a primer combination V and a primer combination VI. The method comprises the following steps: using the DNA of the Chinese cabbage to be detected as the a template, performing PCR amplification with the primer combination of the kit according to the claim 1, detecting the size of a polymorphism segment, and comparing the size of the polymorphism segment with that of a polymorphism segment obtained by carrying out PCR amplification on the existing Chinese cabbage breed with the primer combination; if the segment sizes obtained by the amplification are identical, the Chinese cabbage to be detected and the existing Chinese cabbage belong to the same breed; and if the polymorphism segment of the Chinese cabbage to be detected is different from that of the existing Chinese cabbage obtained by amplification, the Chinese cabbage to be detected is not the same breed as the existing Chinese cabbage. The method is convenient and simple to operate, and can efficiently and accurately identify the authenticity and consistency of the breed of the Chinese cabbage.

The invention provides a situation analysis method and a system of an LSSVM-ARIMA model based on locust optimization and a storage medium, belongs to the field of machine learning and data mining, andis characterized by comprising the following steps: (1) randomly initializing an initial position of a locust group; (2) determining a target function; (3) updating the position; (4) repeating the steps (1) and (2), and outputting c and sigma; (5) establishing an LSSVM model and an ARIMA model; calculating a prediction result y1 (t); (6) determining a low-frequency component Aj (t) and a high-frequency component Dj (t); (7) obtaining a first prediction result y1 (t); (8) calculating a prediction result y2 (t); and (9) fitting the prediction results y1 (t) and y2 (t) to obtain a final situation result y (t). According to the method, a mode of combining the LSSVM and the ARIMA is adopted, and the locust optimization algorithm is utilized to realize parameter optimization on the time-varyingsituation model. Experimental results show that the enterprise safety production situation prediction method established by the invention is effective, and a reliable method is provided for enterprise safety production management situation analysis.

The invention provides hydroformylated fresh goose red blood cells, a preparation method thereof and application of the hydroformylated fresh goose red blood cells to hemagglutination (HA) test for acellular pertussis. Specific conditions of the method for hydroformylating goose red blood cells are that: the goose red blood cells are hydroformylated for 40min by using 1.25 percent glutaraldehyde; the working concentration of hydroformylated goose red blood cells is 0.8 percent; and when the hydroformylated goose red blood cells are used for the HA test for acellular pertussis, a test result which is consistent with a test result obtained by using 0.8 percent fresh goose red blood cells is obtained, but after the fresh goose red blood cells are replaced by the hydroformylated goose red blood cells, the preservation period of the goose red blood cells is prolonged, experimental results are relatively stable, and the disadvantages that 1 percent fresh goose red blood cells which are required to be used in the prior art are crispy and fragile and the preservation period is short are overcome.

The invention discloses a lactate dehydrogenase isoenzyme electrophoretic separation method. Aiming at the defects existing in the separation methods taking barbital as an electrophoretic buffer solution in the prior art, the invention provides the lactate dehydrogenase isoenzyme electrophoretic separation method taking TBE buffer solution as the electrophoretic buffer solution, wherein the TBE buffer solution is obtained by dissolving 10.8g of Tris, 0.744g of Na2EDTA.2H2O and 5.5g of boric acid in distilled water, and making the volume constant to be 1000ml. The method can be optimized from two aspects, namely, controlling the electrophoresis temperature and/or controlling the pH of the electrophoretic buffer solution. The invention further provides a human cancer cell lactate dehydrogenase isoenzyme electrophoretic separation method realized by utilizing the lactate dehydrogenase isoenzyme electrophoretic separation method, and an application method of the TBE buffer solution in the lactate dehydrogenase isoenzyme electrophoretic separation. With the adoption of the method, the technical defects generated due to the use of the barbital buffer solution in the prior art are effectively avoided, and the application values in scientific research, clinical test and experiment teaching of the lactate dehydrogenase isoenzyme electrophoretic separation method are expanded.

The invention discloses a two-dimensional electrophoresis method for separating protein from a kenaf leaf efficiently and stably. In combination of biomass chemical features of the kenaf leaf, on the basis of the conventional protein two-dimensional electrophoresis method, the method for separating protein from the kenaf leaf, the selection of the pH range of an IPG gel strip, the protein sample applying way, the protein sample applying amount, the concentration of a separation gel, the setting of parameters of protein spot analyzing software and other aspects are improved and optimized. By the method, a two-dimensional electrophoretogram of the protein of the kenaf leaf with more clear protein spots which are uniformly distributed and with clear background can be obtained; the experimental result is stable; and averagely, 1300 protein spots can be detected on the gel with the size of 24cm*16cm. The invention paves a working foundation for further proteomic and molecular biologic research on kenaf and other hemps.

The invention relates to a stereo image comfort degree evaluation based on a multi-scale space theory and a non-salient region map. A distortion image pair is set as (Dl, Dr), and the method comprisesthe following steps: 1) computing 2D salient map SSal of the Dr; 2) respectively obtaining a disparity map D with the same size as an original image and two D1/4 disparity maps of down-sampling disparity map with the 1/4 size of the original image; 3) for two disparity maps, respectively extracting disparity statistical features, and placing two groups of feature vectors and a mean opinion scoreMOS into two SVW regression training networks; respectively extracting two groups of disparity statistical features through linear regression training by using two support vectors, and performing iterative training so as to obtain the comfort degree quality score finally.

The invention discloses a preparation method of a novel rare earth red pigment gamma-Ce2S3. The preparation method comprises the following steps: preparing modified CeO2 composite nano precursor powder and calcining and molding the red pigment gamma-Ce2S3. The preparation method of the modified CeO2 composite nano precursor powder comprises the following steps: preparing a CeCl3 solution and a Na2S solution, preparing a CeO2 precursor sample, preparing a CeO2 nano powder precursor and molding the modified CeO2 composite nano powder precursor. The calcining and forming process of the red pigment gamma-Ce2S3 comprises the following steps: calcining the modified composite nano-powder precursor and forming the red pigment gamma-Ce2S3. The red pigment gamma-Ce2S3 prepared by the preparation method disclosed by the invention has the advantages that the tinting strength, glossiness, purity and color intensity are obviously improved, meanwhile, the adhesive force is also obviously enhanced, the experiment result is stable through repeated experiments, and the preparation method is simple and reliable.

The invention relates to the field of biotechnology and particularly discloses a method for researching helicoverpa armigera larva RNA interference efficiency. The method for researching the helicoverpa armigera larva RNA interference efficiency is established by improving a method for feeding helicoverpa armigera larva siRNA (small interfering RNA), the lepidopteron helicoverpa armigera larva RNA interference efficiency is improved, and experimental results are stabilized. The method provided by the invention is efficient and simple, and is high in operability, the dosage of siRNA is definite, the repeatability is high, and technical supports for researching functions of genes of lepidopteron helicoverpa armigera are provided.

The invention provides a rapid seed selection kit for high-oleic acid peanuts. The research results show that fatty acid dehydrogenase gene (FAD2) is the key gene for controlling the oleic acid traitof peanuts, wherein FAD2 is co-encoded by two non-alleles FAD2A and FAD2B located on different genomes. According to the present invention, based on the genetic gene, multiplex amplification primers respectively for wild and mutant FAD2A gene and FAD2B gene markers are designed by using a colloidal gold test paper strip as a carrier according to the gene sequence conserved region in a peanut high-oleic-acid mutant gene so as to optimize the primer combinations and the reaction system; by using the principle of nucleic acid hybridization, the labeled amplification products are bound with the specific antibody on the colloidal gold test paper strip so as to establish the PCR amplification and colloidal gold lateral flow immunoassay hybridization kit for detecting FAD2A gene and FAD2B gene; and the kit has characteristics of simple operation, strong specificity and high detection sensitivity.

The invention relates to a method of metabonomically identifying fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum based on 1H-NMR and belongs to the technical field of identification of traditional Chinese medicines. A fresh corn cervi pantotrichum product and a processed product are identified through a multivariate data processing mode by researching the chemical compound difference of fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum by applying a metabonomic technology of 1H-NMR. Compared with an existing corn cervi pantotrichum identification technology, the method provided by the invention has obvious advantages: metabolites of corn cervi pantotrichum are illustrated integrally, and difference among the metabolites can be compared systematically; by means of progressive PCA, PLS-DA and OPLS-DA layer by layer, the accuracy of an experimental result is ensured; the experimental result can be stable and controllable by means of rich nuclear magnetism data combined with modern high-tech analyzing software, so that the method has macro accuracy and micro precision. The method is also simple, convenient, rapid and economic and suitable for universality and simplicity of market and common individual applications.

The invention discloses a simple preparation method of a rapid PCR template of filamentous fungi. The simple preparation method comprises the following steps of: (1) culturing to-be-detected filamentous fungi to obtain mycelia; (2) picking the mycelia of the filamentous fungi and putting the mycelia into a glass test tube filled with distilled water to obtain a mycelia mixed solution; (3) carrying out ultrasonic treatment on the glass test tube containing the mycelia mixed solution; and (4) directly taking the mycelia mixed solution subjected to ultrasonic treatment as a DNA template to carry out PCR amplification. The method disclosed by the invention does not need liquid nitrogen grinding, does not need to add any extraction reagent, is simple and rapid, and saves time and labor. The defects that existing filamentous fungi are long in DNA extraction time, more in added reagent and more in required instrument are overcome. The DNA template extracted by the method disclosed by the invention can obtain a stable and reliable experimental result through PCR reaction, is low in cost, good in effect, suitable for rapid analysis of large-batch samples, and also suitable for detection in a laboratory with imperfect instrument conditions.

The invention discloses an integrated hypoxia experiment simulation device and a method of mammals. The integrated hypoxia experiment simulation device comprises a processor module; the processor module is connected with a display module, a setting module, a sensor module, a power module and a control module; the control module is connected with a vacuum pump. The integrated hypoxia experiment simulation device provided by the invention has the characteristics of being compact in structure and convenient to use; besides; the device can automatically control vacuum pressure, reduce the operating difficulty of students making experiments, and enable the students to mainly focus on the observation of the hypoxia phenomenon, so that the experiment efficiency is greatly enhanced, and the experiment effect is improved; moreover, the experimental device is stable in experiment effect, high in repeatability and good in effect of experiment teaching.

The invention relates to a method for extracting abundant total DNA (deoxyribonucleic acid) from grape fruits, which comprises the following steps: 1. grinding grape fruits into granules; 2. extracting the granules with sorbitol, and filtering out polysaccharides to obtain grape pomace 1; 3. extracting the grape pomace 1 with mercaptoethanol, and filtering out phenols to obtain grape pomace 2; and 4. extracting the grape pomace 2 with a chloroform-isoamyl alcohol-KAc mixture, and filtering out proteins to obtain grape pomace 3 which is the total DNA in the grape fruits.

The invention provides a method for identifying pathogenicity of fusarium verticillioides indoors, and belongs to the technical field of prevention and treatment of corn diseases. The method comprisesthe following steps of (1) culturing the fusarium verticillioides indoors to obtain a fusarium verticillioides single bacterial colony; (2) cutting off a culture medium block having bacteria along the outer edge of the fusarium verticillioides single bacterial colony obtained in the step (1) to obtain bacterium cakes; and (3) taking fresh corn pistils, enabling the taken fresh corn pistils to beattached and put on the bacterium cakes obtained in the step (2) in parallel, performing airtight standing for 5-9d, observing the pathogenesis situation of the corn pistils, and determining the pathogenicity of the fusarium verticillioides. The method disclosed by the invention is operated indoors, the operation course is simple, the work load is small, and human resources can be saved; besides,in the implementation course, temperature and humidity are easy to control and are not liable to influence by external environment, and the experimental result is accurate, stable, reliable and low inerrors. After verification, the identification result is consistent with the identification result of the pathogenicity of land for growing field crops.

The invention provides a method for effectively extracting genomic DNA (deoxyribonucleic acid) of melaleuca alternifolia, which comprises the following steps: (1) grinding a sample into powder by liquid nitrogen, transferring the powder to a centrifuge tube, adding a DNA extraction buffer which is conventionally preheated, uniformly mixing and carrying out water bath at 65 DEG C for 30 minutes; (2) taking out the centrifuge tube and cooling to room temperature, adding a mixed solution of chloroform and isoamyl alcohol, uniformly mixing and performing centrifugal separation; (3) taking supernate, adding chloroform, uniformly mixing and performing centrifugal separation; (4) taking supernate, adding absolute ethyl alcohol which is conventionally precooled, uniformly mixing, standing for 30 minutes at -20 DEG C, then performing centrifugal separation, and discarding the supernate; (5) washing sedimentation with ethyl alcohol of which the volume concentration is 75%, drying at room temperature and adding a DNA dissolution buffer, and storing at -20 DEG C for standby use. The method provided by the invention is capable of effectively and rapidly extracting the genomic DNA of the melaleuca alternifolia which is high in purity and suitable for PCR detection, and the use of toxic chemicals in the extracting process is reduced.

The invention relates to the technical field of molecular biology experiments, in particular to a plant disease bacteria total DNA extracting method. The plant disease bacteria total DNA extracting method comprises the steps that a bacteria sample is subjected to culture by using a fluid medium, dissolving by using lysozyme, DNA separation by using a CTAB/NaCl solution and multiple chloroform andphenol-chloroform extraction to obtain an extracting solution containing DNA, and impurities in the extracting solution are removed to obtain the disease bacteria total DNA. The DNA extracting methodis good in cell wall breaking effect, the phenol-chloroform layered extraction rate is high, and the method is especially suitable for extracting the DNA of pathogenic bacteria of fruits and vegetables, such as rice, wheat, tomato and cucumber.

The invention relates to the field of biological technology, in particular to a probe of mRNA stem-loop structure and a preparation method thereof as well as application on EMSA thereof. The probe of mRNA stem-loop structure is fabricated according to the following method: according to the sequence of nuclei acid of the objective stem-loop structure, chemical synthesis method is adopted to directly synthesize a probe with the sequence length of nuclei acid of no more than 70nt, then fluorescence mark method is adopted to mark the probe. Applying the probe of stem-loop structure with fluorescence mark into EMSA technology has the advantages of the high specificity, the high sensitivity, the repeatability and the stability, simultaneously the preparation method is simple and the price is low.

The invention discloses a method for determining the content of glucose or a glucose polymer in a compound preparation, and belongs to the technical field of drug analysis and detection. The method comprises: (1) taking a compound preparation, and diluting to prepare a solution to be determined, wherein the dilution factor is Dil; (2) obtaining the concentration Cn of other optically active compounds excluding glucose or a glucose polymer in the solution to be determined; (3) according to an optical rotation determination method, determining the optical rotation [alpha] of the solution to be determined; and (4) calculating the content of the glucose or the glucose polymer according to the formula defined in the specification, wherein Fn is [alpha]n*L*Cn. According to the present invention,the content of the glucose or the glucose polymer in the compound preparation simultaneously containing two or more than two optically active compounds is determined by using the optical rotation determination method,, such that the method is simple, the operation is convenient, the result is stable and accuracy, and the parallelism is good.

The invention discloses a detection device and method for an irrigation water viscosity coefficient based on a magnetostrictive displacement sensor, and belong to the technical field of liquid viscosity coefficient detection. The detection device comprises a triangular iron support, a magnetostrictive displacement sensor, a temperature sensor, a measuring cylinder, a pressure sensor and a data processing and displaying device. The temperature sensor and the pressure sensor which are used for measuring temperature and density are arranged in the device, the influence of the temperature sensor and the pressure sensor on an experimental measurement result is considered, an experimental result is further corrected, and the accuracy is greatly improved. The limitation is greatly reduced, measurement can be carried out after a metal feeler lever of the magnetostrictive displacement sensor extends into the liquid for a certain depth, and the measurement can be carried out in any liquid and atany place as the condition is met. The magnetostrictive displacement sensor outputs continuous signals, and compared with a pulse signal, the signals are stable, the response speed is fast, the precision is high, the transportability is good and the data processing and display device can be easily connected.