Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of metabonomically identifying fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum based on 1H-NMR

A technology of metabolomics and fresh velvet antler, which is applied in the field of traditional Chinese medicine identification, can solve the problems of short analysis time and achieve the effects of simple sample preparation, guaranteed experimental results, and simple methods

Active Publication Date: 2018-04-06
CHANGCHUN UNIV OF CHINESE MEDICINE
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Metabolomics analysis based on nuclear magnetic resonance has the advantages of simple sample preparation, good repeatability, short analysis time, and the ability to detect various polar compounds at the same time. This method has been successfully applied to the analysis of many traditional Chinese medicines, but only donkey-hide gelatin Two kinds of animal drugs, donkey skin and donkey skin, use this analysis method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of metabonomically identifying fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum based on 1H-NMR
  • Method of metabonomically identifying fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum based on 1H-NMR
  • Method of metabonomically identifying fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum based on 1H-NMR

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0072] This implementation case involves a 1 The method of H-NMR metabolomics identification of fresh velvet and hot-fried velvet, the steps included in the method are as follows:

[0073] Step 1: Take fresh Ergang velvet and burn it with alcohol lamp to remove the hair, cut it into 2 parts longitudinally, wrap the section with plastic wrap for 1 part, and use it for traditional hot processing, and 1 part as a fresh product.

[0074] The traditional thermal processing method is: put the wrapped velvet antler into boiling water for boiling and frying for 5 times in total, 1 minute each time; after each frying and frying, insert a 16-gauge needle connected with nitrogen gas into the tip of the wrapped velvet antler. Drain the blood, boil for the fifth time and put it in an oven at 70°C to bake for 8 hours, take it out and cut it into small pieces, wash it with water 5 times, and set it aside.

[0075] Step 2, weighing 20 g of velvet antler sample, crushing the tissue with 10 ti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method of metabonomically identifying fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum based on 1H-NMR and belongs to the technical field of identification of traditional Chinese medicines. A fresh corn cervi pantotrichum product and a processed product are identified through a multivariate data processing mode by researching the chemical compound difference of fresh corn cervi pantotrichum and heat-fried corn cervi pantotrichum by applying a metabonomic technology of 1H-NMR. Compared with an existing corn cervi pantotrichum identification technology, the method provided by the invention has obvious advantages: metabolites of corn cervi pantotrichum are illustrated integrally, and difference among the metabolites can be compared systematically; by means of progressive PCA, PLS-DA and OPLS-DA layer by layer, the accuracy of an experimental result is ensured; the experimental result can be stable and controllable by means of rich nuclear magnetism data combined with modern high-tech analyzing software, so that the method has macro accuracy and micro precision. The method is also simple, convenient, rapid and economic and suitable for universality and simplicity of market and common individual applications.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine identification, in particular to a method based on 1 A method for the identification of fresh velvet and hot-fried velvet by H-NMR proton nuclear magnetic resonance spectroscopy metabolomics technique. Background technique [0002] Deer antler is a traditional precious Chinese medicine, which is the unossified and densely hairy young horns of the male deer Cervus nippon Temminck or the red deer Cervus elaphus Linnaeus. As a traditional clinical medicine, velvet antler has a history of thousands of years in our country. The 2015 edition of "Chinese Pharmacopoeia" records that velvet antler is sweet in taste and warm in nature, strengthens kidney yang, nourishes essence and blood, strengthens muscles and bones, regulates Chong and Ren, and relieves sores. Modern pharmacological studies have shown that deer antler has various pharmacological effects. It can protect myocardial d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
Inventor 孙佳明张辉吴楠刘冬杨银平
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com