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Method for determining biological limit value of Qingjin phlegm-reducing decoction

A measurement method and biological technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., can solve the problems of complex chemical components and few research reports on Qingjin Huatan Decoction

Pending Publication Date: 2021-08-31
安徽济人药业股份有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Due to the complex formula and chemical components of Qingjin Huatan Decoction, through literature search, it was found that there are few research reports on the material standards and quality control of Qingjin Huatan Decoction. Therefore, a biological activity determination method related to its efficacy and pharmacological effects was established , which is of great significance to the quality control of Qingjin Huatan Decoction

Method used

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Embodiment Construction

[0053] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0054] Depend on Figure 1-2 Shown, a kind of Qingjin Huatan Decoction biological limit assay method specifically comprises the following steps:

[0055] Step 1. Prepare the freeze-dried powder solution of the water extract of Qingjin Huatan Decoction and the LPS solution, and cultivate RAW264.7 macrophages;

[0056] Among them, the preparation process of the freeze-dried powder solution of water extract of Qingjin Huatan Decoction is as follows: add the freeze-dried powder of water extract of Qingjin Huatan Decoction in batches 1911260...

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Abstract

The invention discloses a method for determining biological limit value of Qingjin phlegm-reducing decoction, and belongs to the technical field of drug analysis and detection. The method comprises the following steps of Step 1, preparing a Qingjin phlegm-reducing decoction water extract freeze-dried powder solution and an LPS solution, and culturing RAW264.7 macrophages; Step 2, monitoring the relationship between the inoculation density of the RAW264.7 macrophages and incubation time; Step 3, monitoring the influence of the Qingjin phlegm-reducing decoction water extract freeze-dried powder solution on the activity of the RAW264.7 macrophages; Step 4, determining the biological activity limit value of Qingjin phlegm-reducing decoction water extract freeze-dried powder based on the secretion function of the RAW264.7 macrophages; Step 5, determining the biological activity limit value of the Qingjin phlegm-reducing decoction water extract freeze-dried powder based on the phagocytic function of the RAW264.7 macrophages; and Step 6, data processing. By establishing a biological activity determination method for associating the efficacy and pharmacological action of the decoction, the method disclosed by the invention has important significance on quality control of the Qingjin phlegm-reducing decoction.

Description

technical field [0001] The invention belongs to the technical field of drug analysis and detection, and in particular relates to a biological limit value determination method of Qingjin Huatan Decoction. Background technique [0002] Qingjin Huatan Decoction is derived from the "Medical System" written by Ye Wenling in the Ming Dynasty. It consists of 11 flavors including skullcap, gardenia, fritillary, mulberry, melon, orange, bellflower, Ophiopogon japonicus, Anemarrhena, Poria, and licorice. Composed of medicines, it has remarkable effects of clearing away heat and moistening the lungs, resolving phlegm and relieving cough. It is one of the 100 classic famous prescriptions in the "Catalogue of Ancient Classical Famous Prescriptions (First Batch)". In order to fully guarantee and exert its clinical efficacy, it is of great significance to establish a scientific and comprehensive quality control method. The commonly used quality control methods of traditional Chinese medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCG01N33/5055
Inventor 游云张琼玲肖顺丽李文军孙正霄丁世兰李翔宇
Owner 安徽济人药业股份有限公司
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