5 results about "Two dimensional electrophoresis" patented technology

The invention provides a rapid quantitative analysis method for an outer vesicle proteome, and belongs to the technical field of quantitative analysis of the outer vesicle proteome. According to the rapid quantitative analysis method for the outer vesicle proteome, firstly, a high-quality outer vesicle sample is obtained through low-speed centrifugation, ultra-speed centrifugation and membrane filtration; and obtaining proteome data by utilizing two-dimensional electrophoresis and mass spectrometry. Then, a mathematical model equation set is constructed for preliminary analysis, a protein interaction network is optimized through an embedded game optimization index model, and feature extraction and quantitative analysis are conducted through a convolution parameter adaptive function and a residual network deep learning model; finally, a quantitative analysis result containing the protein expression level and the function importance is generated in combination with the multi-dimensional protein expression contribution index, and the technical problem that in the prior art, in the rapid quantitative analysis process of the outer vesicle proteome, the protein component complexity is high, and consequently the quantitative precision is low is solved.

Epitachophoresis (ETP) methods and devices that improve concentrating samples and / or separating components of samples. ETP methods and devices allow for electromigration in two dimensions. Electromigration of a sample may first occur in a first dimension along a single plane. Electromigration may then continue in a second dimension, which may be different from the first dimension. The volume where the electromigration occurs may significantly reduce from the first dimension to the second dimension. This smaller dimension may allow for increased concentration of samples or improved separation of components of a sample.

The application discloses a strip detection method, device, medium and product, relates to the strip detection field, and the method comprises the following steps: carrying out data preprocessing on a two-dimensional electrophoresis image; the data preprocessing comprises the following steps: background removal, background removal and normalization; one-dimensional intensity projection is carried out on the preprocessed image, and one-dimensional intensity curve is generated; the one-dimensional intensity curve is used for representing the overall intensity distribution of each row of pixels in the preprocessed image; the one-dimensional intensity curve is detected peak value, and the search interval of the strip is determined; in the search interval corresponding to each strip, the center curve, the upper boundary curve and the lower boundary curve of the strip are determined by adopting a column-by-column scanning algorithm; and the strip is positioned according to the center curve, the upper boundary curve and the lower boundary curve of the strip. The application can improve the efficiency, accuracy, adaptability and operation convenience of strip detection in electrophoresis experiments.

An object of the present invention is to provide a new marker for prostate cancer and a method for detecting prostate cancer using the marker. The present invention provides a method for determining prostate cancer comprising the following steps of: (a) releasing PSA from a PSA-ACT complex present in a sample derived from a subject; (b) performing two-dimensional electrophoresis on the free PSA obtained in the step (a) to separate PSA and detecting a PSA spot; and (c) verifying the presence of the PSA spot detected on the basic side compared to the control PSA spot.

The invention discloses a strip detection method and device, a medium and a product, and relates to the field of strip detection, and the method comprises the steps: carrying out the data preprocessing of a two-dimensional electrophoresis image; the data preprocessing comprises the steps of background removal, background removal and normalization; performing one-dimensional intensity projection on the preprocessed image to generate a one-dimensional intensity curve; the one-dimensional intensity curve is used for representing the overall intensity distribution of each row of pixels in the preprocessed image; carrying out peak value detection on the one-dimensional intensity curve, and determining a search interval of a stripe; determining a center curve, an upper boundary curve and a lower boundary curve of the strip by adopting a column-by-column scanning algorithm in the search interval corresponding to each strip; and carrying out strip positioning according to the center curve, the upper boundary curve and the lower boundary curve of the strip. According to the invention, the efficiency, accuracy, adaptability and operation convenience of strip detection in an electrophoresis experiment can be improved.