Two-dimensional electrophoresis method for separating protein from kenaf leaf efficiently and stably

A two-dimensional electrophoresis and protein technology, applied in the field of plant molecular biology, can solve the problem of late start of kenaf proteomics research, and achieve the effects of stable experimental results, large numbers and uniform distribution.
CN102382166AInactive Publication Date: 2012-03-21FUJIAN AGRI & FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUJIAN AGRI & FORESTRY UNIV
Publication Date
2012-03-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a two-dimensional electrophoresis method for separating protein from a kenaf leaf efficiently and stably. In combination of biomass chemical features of the kenaf leaf, on the basis of the conventional protein two-dimensional electrophoresis method, the method for separating protein from the kenaf leaf, the selection of the pH range of an IPG gel strip, the protein sample applying way, the protein sample applying amount, the concentration of a separation gel, the setting of parameters of protein spot analyzing software and other aspects are improved and optimized. By the method, a two-dimensional electrophoretogram of the protein of the kenaf leaf with more clear protein spots which are uniformly distributed and with clear background can be obtained; the experimental result is stable; and averagely, 1300 protein spots can be detected on the gel with the size of 24cm*16cm. The invention paves a working foundation for further proteomic and molecular biologic research on kenaf and other hemps.
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Description

technical field

[0001] The invention belongs to the field of plant molecular biology and relates to a kenaf proteomics technology, in particular to a stable and efficient two-dimensional electrophoresis method for separating proteins from kenaf leaves. Background technique

[0002] Kenaf (Kenaf) is Malvaceae (Malvaceae) Hibiscus genus ( Hibiscus ) annual bast fiber crop, which was cultivated in African Sudan in 4000 BC. As a natural fiber crop, kenaf has the characteristics of fast growth, strong stress resistance, and wide adaptability. The huge biological yield is 3- 4 times, the extremely strong carbon dioxide absorption capacity is 4-5 times that of forests, and it is an advantageous crop for developing a low-carbon economy. In addition to being traditionally used as raw materials for processing hemp rope, sacks, carpets, etc., kenaf fiber is also a good material for processing automotive interiors, panels, and hemp carbon. Antibacterial and other biological functions....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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