33 results about "Bioindicator" patented technology

A sensor and method for detecting biological and chemical agents comprising metal interdigitized electrodes coated with hybrid polymer-based conducting film and an instrument for applying electrical voltage to the electrodes and registering the change in electrical current. The hybrid film also comprises indicator biomolecules encapsulated within the film or attached to it. The bioindicator molecules preferably comprise enzyme acetylcholinesterase. When these indicator biomolecules come in a contact with a pathogen, chemical and/or morphological changes occur in the film and electrical current flowing through the electrodes is modulated. The pathogen comprise inhibitors of enzymes, preferably organophosphates, thiophosphates or phosphonates. The change in current indicates the presence of a biological and chemical agent and is registered.

The present invention relates to novel types of cellular calcium probes that are based on Troponin C and two chromophors suitable for FRET (fluorescence resonance energy transfer). The Troponin C-based calcium sensors of the invention function in diverse subcellular environments, for example even when tethered to a cellular membrane. The invention further provides nucleic acid constructs encoding the calcium probes of the invention, expression constructs, host cells and transgenic animals. Furthermore, methods for the detection of changes of local calcium concentrations and for detecting the binding of a small molecule to fragments of Troponin C are provided.

A biological indicator including a carrier and an indicator material, said indicator material adhering on the surface of the carrier, and having a contact portion being contact with the surface of the carrier and a non-contact portion being not in contact with the surface of the carrier, and wherein the carrier includes an adhering area having a adhering portion to which the indicator material adheres and a non-adhering area having an non-adhering portion to which the indicator material does not adhere.

A biological indicator for monitoring the effectiveness of a sterilizing, disinfecting, etc., compound or condition is disclosed which comprises a substrate having a surface layer containing functional groups thereon desirably free of silicone linking groups. The functional groups are desirably in the form of a monolayer of a uniformed distribution and of a selected quantity. Various types of microorganisms such as spores and/or etiological agents are covalently bonded to the surface layer functional groups through a crosslinking reagent and thus form a uniform number and distribution of the microorganisms and/or etiological agents. After being subjected to sterilization or other similar treatment, etc., along with various articles such as instruments, the indicator can be cultivated to determine the effectiveness of the sterilization, disinfection, etc. process.

A biological indicator and method of making same. The biological indicator includes a carrier having a recess formed therein in order to restrict movement of an inoculum deposited onto the carrier. The inoculum includes microorganisms (e.g., bacterial spores) suspended in a suspension medium. The microorganisms are prepared by removing extraneous material and subjecting the microorganisms to sonication to break up agglomerations. The suspension medium includes a wetting agent to reduce surface tension, thereby facilitating flow of the suspension medium to prevent stacking of microorganisms on the surface of the carrier, and to allow the inoculum to more evenly “plate out” on carrier surfaces. The carrier, with inoculum deposited thereon, is enclosed in an envelope made of a material permeable to a vaporous deactivating agent (e.g., vaporized hydrogen peroxide, ozone, chlorine dioxide, ethylene oxide, etc.), thereby facilitating exposure to the vaporous deactivating agent.

In a method of determining the effectiveness of a sterilization procedure using a biological indicator, the biological indicator comprises a holographic sensor and spores or an enzyme. The method and the biological indicator rely on the holographic sensor to indicate the effectiveness of me sterilisation procedure and do not require a conventional pH indicator, a fluorophore or chromophore.

The present invention provides a test medium comprising a test microorganism, an indicator and a metal in ionized form, wherein the valence of said metal is at least 2 and the concentration of said metal is between 0.001 M and 1 M. Furthermore, there is provided a method for the determination of the presence of an antibiotic characterized in that a metal salt is added to said test medium and/or to said sample of fluid. Finally, there is provided a kit suitable for the determination of an antibiotic in a fluid.

The invention relates to a detection reagent kit and method of heat-resistant microorganisms in elemene lipidosome injection semi-finished products and belongs to the technical field of medical instruments and detection means. The detection reagent kit contains sterile elemene standard injection and heat-resistant microorganism biological indicator. The detection steps include S1 screening heat-resistant microorganisms in the elemene lipidosome injection semi-finished products by the boiling water bath; S2, separating, identifying and storing heat-resistant bacteria of bacillus; S3, preparing spore liquid of the heat-resistant bacteria prepared in the step S2, and calibrating concentration of spore liquid; S4, preparing biological indicator by products obtained in the step S3, and calibrating concentration of the biological indicator; S5, boiling quantitatively. The detection reagent kit fills the blank of the related technology and forms the detection technology of a complete closed loop from inspection, evaluation and treatment of heat-resistant microorganisms. The detection reagent kit helps detectors detect the pollution state of elemene, labor is saved, and detection efficiency is improved.

The application discloses a multifunctional biomedical instrument, which includes a main machine, an ozone generator, a cover body and a bottom plate. The main machine includes a base and a functional cabin arranged on the base; the ozone generator is located in the base; the main machine is placed on the bottom plate; the cover is closed on the bottom plate to form a closed space, and the base, functional cabin and ozone generator are all located in the closed space. Thus, a closed space is formed by the cover body and the bottom plate, the working host is placed in the closed space, and then disinfected by the ozone generator, so that the working host can be ozone-sterilized before or after each work is completed, ensuring that the operating samples And the working environment is free from pollution. By cooperating with different functional components, the working host can realize various functions such as dry recovery of cryopreserved cells, constant temperature culture of microorganisms, constant temperature oscillation of microwell plates, rocker shaker, metal bath, constant temperature culture of biological indicators, cell heat shock culture, etc. .

During autoclave sterilization, technicians often tighten waste bags too tight not allowing proper steam permeation into the items in the autoclave bag. Additionally, biological indicators (bioindicators) should be used to confirm sterilization of items in the waste bag; however it is recommended that these indicators be placed close to the center of the bag to give a more accurate representation of sterilization. This is not safely feasible due to the need to probe into a waste bag for placement and retrieval of biological indicators after autoclaving. This device addresses both of these technical problems. Placing it in an autoclave bag cinched at the composite collar allows steam to penetrate into the autoclave bag via a pass-through tube close to the center mass of the waste bag. Additionally, this device holds many types of biological indicators allowing them to be safely placed and retrieved from center mass of an autoclave bag.

The invention relates to a biological indicator for sterilization of medical instruments, in particular to a container for the biological indicator. The container comprises an outer pipe and a cap. The cap is mounted at the upper end of the outer pipe; a cap through hole communicated with a pipe cavity of the outer pipe is formed in the cap; an outer pipe end wall is arranged at the lower end of the outer pipe; and the outer pipe end wall is provided with an end wall through hole communicated with the pipe cavity of the outer pipe. The end wall through hole is formed in the outer pipe end wall, so that the end wall through hole, the pipe cavity of the outer pipe and the cap through hole are communicated to form a smooth gas flow path; the gas flow path is used for circulation of steam in the disinfection process of a pressure steam sterilizer; and the steam circulation effect of the outer pipe can be improved by utilizing the convection principle, so that bacterial flakes in the container can be in more sufficient contact with the steam, and the indication of the biological indicator is more accurate.

This invention relates to a biological indicator, comprising: a carrier inoculated with a test organism and an effective amount of a carbohydrate to reduce the resistance of the biological indicator to steam sterilization. The invention relates to a steam sterilization process using the biological indicator.

The invention relates to a biological indicator for determining the efficacy of a steam or heat sterilization process, and its method. The biological indicator comprises microbial spores (a), at least one sensor protein exogenous to the microbial spores (b), one fluorophore (c), and a culture medium (d). The invention also refers to the method of use of this biological indicator. This method consists of (a) placing the biological indicator along with a target material to be steam or heat sterilized within a steam or heat sterilizer, (b) carrying out a steam or heat sterilization process, (c) placing the biological indicator in a an incubator, (d) screening the biological indicator for immediate detectable changes in fluorescence intensity, while incubating the biological indicator in the incubator, (e) determining the efficacy of the steam or heat sterilization process based on the screening carried out during step d), (f) extending the incubation of the biological indicator obtained in step d), (g) screening the incubated biological indicators obtained in step e) for an optically detectable color change, and (h) determining the efficacy of the steam or heat sterilization process, according to optically detectable changes obtained in step g).

The invention discloses a reuse dressing sterilization validation device for fast comprehensive challenge of pressure steam sterilization. The reuse dressing sterilization validation device consists of reuse dressing load, an aluminum alloy housing, a sealing rubber ring and a fast bioindicator, wherein the fast bioindicator consists of a fifth-category pressure steam chemical indicator, a transparent bottle, a breathable cap, an ampoule bottle, an ampoule bottle holder, a bacteria tablet and a first-category chemical indicator; the reuse dressing load is arranged in the aluminum alloy housing; the fast bioindicator is arranged in the reuse dressing load; and the sealing rubber ring sleeves the aluminum alloy housing. The reuse dressing sterilization validation device has the advantages that the sterilization effect of a real dressing instrument set can be achieved; and two monitoring means of five categories of chemical indicators and the bioindicator can be synchronously carried out,so that the cost is reduced, the environment is protected, standardization of the monitoring means is also achieved, the industry standard requirements are met, and the complete device can be reusedto reduce the challenge cost.