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Method for identifying breeds of Chinese cabbage and special kit thereof

A variety identification and kit technology, applied in biochemical equipment and methods, microbial determination/inspection, instruments, etc., can solve problems such as time-consuming and financial resources, and achieve high polymorphism, stable experimental results, and repeatability. Good results

Inactive Publication Date: 2010-01-06
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acquisition of effective SSR primers consumes a lot of time and financial resources

Method used

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  • Method for identifying breeds of Chinese cabbage and special kit thereof
  • Method for identifying breeds of Chinese cabbage and special kit thereof
  • Method for identifying breeds of Chinese cabbage and special kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, acquisition of special kit for identification of Chinese cabbage variety and verification of its effect

[0033] 1. Materials and methods:

[0034] 1. Plant material

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Abstract

The invention discloses a method for identifying breeds of Chinese cabbage and a special kit thereof. The kit comprises a primer combination I, a primer combination II, a primer combination III, a primer combination IV, a primer combination V and a primer combination VI. The method comprises the following steps: using the DNA of the Chinese cabbage to be detected as the a template, performing PCR amplification with the primer combination of the kit according to the claim 1, detecting the size of a polymorphism segment, and comparing the size of the polymorphism segment with that of a polymorphism segment obtained by carrying out PCR amplification on the existing Chinese cabbage breed with the primer combination; if the segment sizes obtained by the amplification are identical, the Chinese cabbage to be detected and the existing Chinese cabbage belong to the same breed; and if the polymorphism segment of the Chinese cabbage to be detected is different from that of the existing Chinese cabbage obtained by amplification, the Chinese cabbage to be detected is not the same breed as the existing Chinese cabbage. The method is convenient and simple to operate, and can efficiently and accurately identify the authenticity and consistency of the breed of the Chinese cabbage.

Description

technical field [0001] The invention relates to a method for identifying varieties of Chinese cabbage and a special kit thereof. Background technique [0002] Cabbage vegetables are popular vegetables originating in my country, and their cultivation area is widely adapted to cultivation under various climatic conditions in the whole country. Since the difference in varieties directly affects the production and use performance of such vegetables, the identification of varieties is particularly important. For the identification of commercial seed varieties for controlling seed quality, variety testing for new variety registration, and genetic diversity analysis of resource germplasm, it is very necessary to develop an efficient, fast, accurate, economical and practical method for variety identification of cabbage vegetables. [0003] The classic identification method is the field detection of morphological markers (Meng Shuchun, Zheng Xiaoying, Liu Yumei, He Weiming, Liu Pang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/447
Inventor 李丽郑晓鹰宋顺华吴萍马连平张风兰李秀清邢宝田
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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