Method for improving RNA interfering efficiency of mandibulate insects

Abstract

The invention provides a method for improving RNA interfering efficiency of mandibulate insects, comprising: 1), preparing target gene specific siRNA into a solution of certain concentration using water free of RNA enzyme; 2), processing artificial feed into discs 3-5 mm in thickness and 1-2 cm in diameter, and placing the discs in a 24-pore plate; 3), adding the solution of step 1) to the discs of step 2) so that the solution is absorbed by the feed completely; 4), placing newly hatched larvae of mandibulate insects into the 24-pore plate with the feed discs of step 3), feeding for a period of time, and detecting target gene expression of the insects. By modifying the method of feeding insect siRNA, the method for improving RNA interfering efficiency of mandibulate insects is established; the method is efficient and simple and highly operable, usage of siRNA is saved, interfering effect is significant, and technical support is provided for studying the functionality of genes of mandibulate insects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for improving RNA interference efficiency of chewing mouthpart insects. Background technique [0002] Small molecular weight RNA (smallRNA) is a class of non-coding RNA molecules with a length of about 20-30 nucleotides (nt). Since it was first discovered in nematodes in 1995, it has attracted more and more attention from relevant scientists because of its special biological functions. According to the synthetic route, small molecular weight RNA can be divided into two types: small molecular weight interfering RNA (small interfering RNA, siRNA) and microRNA (microRNA, miRNA). When some small molecular weight endogenous or exogenous double-stranded RNA (double-strandedRNA, dsRNA) molecules are introduced into the host cell, they will induce specific degradation of the homologous endogenous mRNA, thereby efficiently and specifically It blocks the expression activity of homol...

AI Technical Summary

Problems solved by technology

The more commonly used methods of introducing siRNA into insects are mainly injection and feeding methods. The injection method requires special equipment, and the mechanical damage of the injection itself will cause a high mortality rate of the insects; the feeding method is relatively simple, but It has the characteristics of slow action and low efficiency

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