Method for gene transformation for barbadosnut by utilizing kanamycin resistant gene as screening mark

A kanamycin and selection marker technology, applied in the field of tissue culture and genetic engineering of Jatropha curcas, can solve the problems of impracticality and low success rate, avoid a large number of molecular detection work in the later stage, and achieve a high differentiation rate. , to ensure the effect of normal growth and differentiation
CN102304543BInactive Publication Date: 2014-05-28SINO FOREST CHINA INVESTMENTS LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SINO FOREST CHINA INVESTMENTS LTD
Publication Date
2014-05-28
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The invention relates to a method for gene transformation for barbadosnut by utilizing a kanamycin resistant gene as a screening mark. The method comprises the following steps: preparing a barbadosnut explant and an agrobacterium liquid, and carrying out cocultivation on the explant after impregnation by agrobacterium; cultivating the explant after the explant is subjected to cocultivation and bacteria removal and put in a screening culture medium C13KC, thus resistance callus is obtained; transferring the resistant callus to a differential medium SR13KC, and differentiating to obtain resistant adventitious buds; and transferring the resistant adventitious buds to a rooting medium R2KC, thus a transgenosis plant is obtained. Through utilizing the method provided by the invention, the genetic transformation operation to the barbadosnut by utilizing agrobacterium mediation is simple and convenient, the cost is low, and the method is used for establishing a favorable foundation for the barbadosnut genetic modification by an imported-function gene and the acquisition of high-yield high-oil and chill-proof novel products and the like.
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Description

technical field

[0001] The invention relates to the technical field of tissue culture of Jatropha curcas and the field of genetic engineering, in particular to a method for gene transformation of Jatropha curcas by using kanamycin as a selection marker. Background technique

[0002] The energy crisis is a huge challenge facing the world today. China's oil reserves only account for 2% of the world's total, but its consumption ranks second in the world. Therefore, there is an urgent need to find a new type of energy to replace the currently widely used fossil energy.

[0003] Compared with fossil resources, growing energy plants to obtain biodiesel has the advantage of being renewable, which is the fundamental way to solve the energy crisis. The energy plants that have been discovered so far are mainly concentrated in Apocynaceae, Euphorbiaceae, Romoaceae, Compositae, Myrtaceae, and Fabaceae.

[0004] Jatropha ( Jatopha curcas L.), also known as Jatropha, Diesel tree, belo...

Claims

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