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Method of acquiring plant phloem

A phloem and plant technology, applied in the field of obtaining plant phloem, can solve problems such as increasing difficulty, and achieve the effects of high cell homogeneity and accurate positioning

Inactive Publication Date: 2015-04-01
BEIJING UNIV OF AGRI
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  • Abstract
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Problems solved by technology

[0004] With the development of micromanipulation, Laser Capture Microdissection (LCM), which was originally applied in medicine, was applied to the research of botany. Compared with animal cells, plant cells are very different, with cell walls And vacuoles, especially dicotyledonous plants have secondary growth characteristics, and there is secondary thickening of the cell wall, which increases the difficulty of tissue sample preparation

Method used

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  • Method of acquiring plant phloem
  • Method of acquiring plant phloem
  • Method of acquiring plant phloem

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Embodiment 1

[0031] Embodiment 1, the combination of paraffin section and laser microdissection (Laser Capture Microdissection, LCM) accurately obtains the phloem of tomato stem

[0032] 1. Preparation of Paraffin Sections

[0033] 1. Material planting: Tomato seeds are treated by soaking and accelerating germination. After 2 to 3 days, when 60% of the seeds are "broken and white", that is, when the radicle is exposed, they are sown in the seedling tray. The seedling substrate is high-temperature sterilized peat: vermiculite (mass ratio 1:1). The tomato seedlings are placed in a greenhouse at a cultivation temperature of 25-28°C, 16hr light / 8hr dark, and the soil humidity is kept at 50-60%. MS nutrient solution was supplied every two weeks. When the seedlings grow to 2-3 leaves, the seedlings are transplanted into 8×8cm nutrient pots for seedling cultivation.

[0034] 2. Take vigorous tomato seedlings, cut off the leaves, leave only the stem section, and get the strong stem section belo...

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Abstract

The invention discloses a method of acquiring plant phloem. According to the method of acquiring the plant phloem disclosed by the invention, a laser capture microdissection method is adopted to a paraffin section of plant tissues of the phloem to acquire the phloem in the plant tissues. The method comprises the following step: with the combination of the paraffin section and a laser capture microdissection technology (Laser Capture Microdissection, LCM), successfully separating the pure plant phloem of tomatoes. Compared with the previous phloem cell acquiring method, the method is accurate in location and high in cell homogeneity and lays the foundation of molecular biology and genomics research for studying the phloem.

Description

technical field [0001] The invention relates to a method for obtaining plant phloem and belongs to the field of biotechnology. Background technique [0002] There are various RNA molecules in plant phloem sieve molecules. As a new signal regulation mode in plants, it can be transported through the phloem for long distances. This is a breakthrough discovery in the plant world. As the transport organization of vascular plants, the phloem is mainly responsible for transporting the products of photosynthesis from the source organ (source) for photosynthesis to other parts, namely the sink part. Previous studies have proved that there are thousands of RNAs in the phloem, but only a small part has been verified for its function. The regulatory mechanism for the long-distance transport of some RNAs is still unclear. Cytological and molecular biology methods are used to study the phloem. Material transport and gene expression and regulation are scientific problems that scientists h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 王绍辉范敬伟赵文超杨瑞赵福宽王建立
Owner BEIJING UNIV OF AGRI
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