A method for constructing wild rice chromosome segment replacement line

A technology of chromosome fragments and replacement lines, which is applied in the field of construction of wild rice chromosome fragment replacement lines, can solve the problems of too large imported fragments, low efficiency of gene cloning, insufficient research and analysis, etc., and achieve the effect of improving efficiency
CN106191046BActive Publication Date: 2019-05-28INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2019-05-28

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Abstract

The invention discloses a construction method of a wild rice chromsome segment substitution line (CSSL). According to the construction method of the wild rice chromsome segment substitution line, a substitution line population capable of covering an entire chromosome genome is constructed, genetic backgrounds of substitution lines are very clear, for one substitution line, the genetic background of the substitution line and a receptor parent are approximately the same, and a difference with a donor parent exists only in one known marker segment; for a complete set of substitution line, all wild rice chromsome segments have corresponding marking segments, and duplications and deletions are avoided. The constructed wild rice chromsome segment substitution line provides a basis for building a platform for comprehensively conducting wild rice genomics research and long-term beneficial help is provided for rice genomics research and innovation germplasm resources.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for constructing a chromosome segment replacement line of wild rice. Background technique

[0002] Common wild rice (Oryza.rufipogon Griff.) is the ancestor species of Asian cultivated rice (O.sativa L.), and it is an important germplasm resource for rice variety improvement. It has been found that wild rice has valuable genes such as resistance to diseases and insect pests, stress resistance, high yield, high quality, efficient phosphorus utilization, sterility and recovery. Therefore, excavating from wild rice the excellent genes that have been lost or weakened in cultivated rice, and finding and utilizing new favorable gene resources has become a new breakthrough in rice variety improvement.

[0003] Many important agronomic traits such as crop yield, quality, and resistance are mostly complex quantitative genetic traits. Due to its complex genetic backgroun...

Claims

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