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Selenium-enriched yeast rejuvenation method based on activity of selenophosphate synthetase (SPS)

A technology of selenium-enriched yeast and synthase, applied in the field of yeast, can solve the problem that selenium-enriched yeast cannot directly reflect the uncertainty of the ability of inorganic selenium to convert into organic selenium, and achieves the guarantee of excellent strains, a simple rejuvenation process, and a rejuvenation result. directional effect

Active Publication Date: 2020-01-14
安徽省华信生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the problems that the existing rejuvenation method of selenium-enriched yeast cannot directly reflect the ability of inorganic selenium to be converted into organic selenium, and the uncertainty of whether the screened bacterial strain can convert inorganic selenium into organic selenium at a high level, the present invention provides a Rejuvenation method of selenium-enriched yeast based on selenophosphate synthase activity

Method used

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  • Selenium-enriched yeast rejuvenation method based on activity of selenophosphate synthetase (SPS)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The specific operation steps of a selenium-enriched yeast based on selenium phosphate synthase activity are as follows:

[0036] Step 1: Activation of strains

[0037] Use an inoculation needle to pick a ring of selenium-enriched yeast strains to be rejuvenated and inoculate it on a solid medium slope composed of 130 g / L wort powder, 0.1 g / L chloramphenicol, and 15 g / L agar, and activate at 28 ℃ 24 h; then use the inoculation needle to pick up a loop of activated bacteria and place it in a 250 mL shake flask containing 50 mL of liquid medium composed of wort powder 130 g / L and chloramphenicol 0.1 g / L at 28°C Shake culture for 12 hours to obtain activated bacteria liquid;

[0038] Step 2: Hydrogen peroxide (H 2 O 2 ) Primary screening treatment

[0039] Take the activated bacteria solution and inoculate it with 10% of the inoculum amount into 50 mL liquid medium composed of 130 g / L wort powder and 0.1 g / L chloramphenicol, and shake culture at 28 ℃ for 6 h; add hydrogen peroxide...

Embodiment 2

[0044] The specific operation steps of a selenium-enriched yeast based on selenium phosphate synthase activity are as follows:

[0045] Step 1: Activation of strains

[0046] Use an inoculating needle to pick up a loop of selenium-enriched yeast strains to be rejuvenated on the slope of the solid medium, and activate them at 28 ℃ for 24 h; then use the inoculating needle to pick up a loop of activated strains in 50 mL of liquid culture medium In a 250 mL shake flask, shake culture at 28°C for 16 hours to obtain an activated bacterial solution;

[0047] Step 2: Hydrogen peroxide (H 2 O 2 ) Primary screening treatment

[0048] Take the activated bacteria solution and inoculate it in 50 mL liquid medium composed of 130 g / L wort powder and 0.1 g / L chloramphenicol at a 10% inoculum amount, and shake culture at 28 ℃ for 8 h; add hydrogen peroxide to make The final concentration of hydrogen peroxide is 2.5% by volume percentage. Continue to culture for 24 hours to obtain the culture solutio...

Embodiment 3

[0053] The specific operation steps of a selenium-enriched yeast based on selenium phosphate synthase activity are as follows:

[0054] Step 1: Activation of strains

[0055] Use an inoculating needle to pick up a loop of selenium-enriched yeast strains to be rejuvenated on the slope of the solid medium, and activate them at 28 ℃ for 24 h; then use the inoculating needle to pick up a loop of activated strains in 50 mL of liquid culture medium In a 250 mL shake flask, shake culture at 28°C for 15 hours to obtain an activated bacterial solution;

[0056] Step 2: Hydrogen peroxide (H 2 O 2 ) Primary screening treatment

[0057] Take the activated bacteria solution and inoculate it in 50 mL liquid medium composed of 130 g / L wort powder and 0.1 g / L chloramphenicol at a 10% inoculum amount, and culture it with shaking at 28 ℃ for 7 hours; add hydrogen peroxide to make The final concentration of hydrogen peroxide is 2.3% by volume percentage. Continue to culture for 24 hours to obtain the c...

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Abstract

The invention relates to a selenium-enriched yeast rejuvenation method based on activity of selenophosphate synthetase (SPS) and belongs to the technical field of yeast. The selenium-enriched yeast rejuvenation method comprises specific operation steps as follows: (1) activation of strains; (2) H2O2 primary screening treatment; and (3) secondary screening of selenium-enriched yeast with high SPS activity. The capacity of rejuvenated selenium-enriched yeast strains for enriching organic selenium is larger than 2000 [mu]g / g thalli by dry weight, and excellent strain guarantee is provided for fermentation production for converting inorganic selenium into organic selenium by the selenium-enriched yeast at a high level. Compared with an existing selenium-enriched yeast strain rejuvenation method, the selenium-enriched yeast rejuvenation method can directly screen out yeast strains for converting the inorganic selenium into the organic selenium at a high level with the activity of the SPS asa screening index, and the problems that existing rejuvenation methods cannot directly judge organic selenium enrichment capacity and cause rejuvenation failure easily in a rejuvenation process are solved; and by use of H2O2 as a selectivity factor, the workload is greatly reduced, and the uncertainty of organic selenium enrichment capacity of survival strains when the inorganic selenium is usedas the selectivity factor is avoided.

Description

Technical field [0001] The invention belongs to the technical field of yeast, and specifically relates to a method for rejuvenating selenium-enriched yeast. Background technique [0002] Selenium is located in the VI group of the periodic table. It is an essential trace element for the human body. It has biological activities such as anti-oxidation, improving immunity, anti-aging, etc. It can be found in the central nervous system, male reproductive system, endocrine system, exercise system, heart It plays an important role in the maintenance of functional homeostasis such as the vascular system. Long-term lack of selenium in the human body can easily lead to Keshan disease, Kashin-Beck disease, diabetes, cardiovascular and cerebrovascular diseases, neurodegenerative diseases and other diseases. Selenium exists in nature in the form of inorganic selenium and organic selenium. Inorganic selenium is more toxic and easily harmful to human health; while the toxicity of organic seleni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12Q1/04G01N33/573
CPCC12N1/38C12Q1/04G01N33/573
Inventor 杨思林张玉英魏春厅
Owner 安徽省华信生物药业股份有限公司
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