A rejuvenation method for selenium-rich yeast based on selenophosphate synthetase activity
A technology of selenium-enriched yeast and synthase, applied in the field of yeast, can solve the problem that selenium-enriched yeast cannot directly reflect the uncertainty of the ability of inorganic selenium to convert into organic selenium, and achieves the guarantee of excellent strains, a simple rejuvenation process, and a rejuvenation result. directional effect
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Embodiment 1
[0035] A kind of rejuvenation of selenium-enriched yeast based on the activity of selenophosphate synthase The specific operation steps are as follows:
[0036] Step 1: Activation of the strain
[0037] Use an inoculation needle to pick a ring of selenium-enriched yeast strains to be rejuvenated and inoculate it on a solid medium slope composed of 130 g / L wort powder, 0.1 g / L chloramphenicol, and 15 g / L agar, and activate it at 28 °C 24 h; then use an inoculation needle to pick up a ring of activated strains and place them in a 250 mL shake flask containing 50 mL of liquid medium consisting of wort powder 130 g / L and chloramphenicol 0.1 g / L, at 28 °C Shaking culture for 12 hours to obtain activated bacterial liquid;
[0038] Step 2: Hydrogen peroxide (H 2 o 2 ) primary screening treatment
[0039] The activated bacterial liquid was taken and inoculated into 50 mL of liquid medium composed of wort powder 130 g / L and chloramphenicol 0.1 g / L with 10% inoculum, and cultured wi...
Embodiment 2
[0044] A kind of rejuvenation of selenium-enriched yeast based on the activity of selenophosphate synthase The specific operation steps are as follows:
[0045] Step 1: Activation of the strain
[0046] Pick up a ring of selenium-enriched yeast strains to be rejuvenated with an inoculation needle and inoculate it on the slant of the solid medium, and activate it at 28 °C for 24 h; In a 250 mL shake flask, shake culture at 28°C for 16 h to obtain the activated bacterial liquid;
[0047] Step 2: Hydrogen peroxide (H 2 o 2 ) primary screening treatment
[0048] The activated bacterial liquid was taken and inoculated into 50 mL of liquid medium composed of wort powder 130 g / L and chloramphenicol 0.1 g / L with 10% inoculation amount, and cultured with shaking at 28 °C for 8 h; hydrogen peroxide was added to make the The final concentration of hydrogen peroxide was 2.5% by volume, and the culture was continued for 24 hours to obtain the culture solution; the culture solution was ...
Embodiment 3
[0053] A kind of rejuvenation of selenium-enriched yeast based on the activity of selenophosphate synthase The specific operation steps are as follows:
[0054] Step 1: Activation of the strain
[0055] Pick up a ring of selenium-enriched yeast strains to be rejuvenated with an inoculation needle and inoculate it on the slant of the solid medium, and activate it at 28 °C for 24 h; In a 250 mL shake flask, shake culture at 28°C for 15 h to obtain the activated bacterial liquid;
[0056] Step 2: Hydrogen peroxide (H 2 o 2 ) primary screening treatment
[0057] The activated bacterial liquid was taken and inoculated into 50 mL of liquid medium composed of wort powder 130 g / L and chloramphenicol 0.1 g / L with 10% inoculation amount, and cultured with shaking at 28 °C for 7 h; hydrogen peroxide was added to make the The final concentration of hydrogen peroxide was 2.3% by volume, and the culture was continued for 24 hours to obtain the culture solution; the culture solution was ...
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