A rejuvenation method for selenium-rich yeast based on selenophosphate synthetase activity

A technology of selenium-enriched yeast and synthase, applied in the field of yeast, can solve the problem that selenium-enriched yeast cannot directly reflect the uncertainty of the ability of inorganic selenium to convert into organic selenium, and achieves the guarantee of excellent strains, a simple rejuvenation process, and a rejuvenation result. directional effect

Active Publication Date: 2021-03-23
安徽省华信生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the problems that the existing rejuvenation method of selenium-enriched yeast cannot directly reflect the ability of inorganic selenium to be converted into organic selenium, and the uncertainty of whether the screened bacterial strain can convert inorganic selenium into organic selenium at a high level, the present invention provides a Rejuvenation method of selenium-enriched yeast based on selenophosphate synthase activity

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  • A rejuvenation method for selenium-rich yeast based on selenophosphate synthetase activity

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Embodiment 1

[0035] A kind of rejuvenation of selenium-enriched yeast based on the activity of selenophosphate synthase The specific operation steps are as follows:

[0036] Step 1: Activation of the strain

[0037] Use an inoculation needle to pick a ring of selenium-enriched yeast strains to be rejuvenated and inoculate it on a solid medium slope composed of 130 g / L wort powder, 0.1 g / L chloramphenicol, and 15 g / L agar, and activate it at 28 °C 24 h; then use an inoculation needle to pick up a ring of activated strains and place them in a 250 mL shake flask containing 50 mL of liquid medium consisting of wort powder 130 g / L and chloramphenicol 0.1 g / L, at 28 °C Shaking culture for 12 hours to obtain activated bacterial liquid;

[0038] Step 2: Hydrogen peroxide (H 2 o 2 ) primary screening treatment

[0039] The activated bacterial liquid was taken and inoculated into 50 mL of liquid medium composed of wort powder 130 g / L and chloramphenicol 0.1 g / L with 10% inoculum, and cultured wi...

Embodiment 2

[0044] A kind of rejuvenation of selenium-enriched yeast based on the activity of selenophosphate synthase The specific operation steps are as follows:

[0045] Step 1: Activation of the strain

[0046] Pick up a ring of selenium-enriched yeast strains to be rejuvenated with an inoculation needle and inoculate it on the slant of the solid medium, and activate it at 28 °C for 24 h; In a 250 mL shake flask, shake culture at 28°C for 16 h to obtain the activated bacterial liquid;

[0047] Step 2: Hydrogen peroxide (H 2 o 2 ) primary screening treatment

[0048] The activated bacterial liquid was taken and inoculated into 50 mL of liquid medium composed of wort powder 130 g / L and chloramphenicol 0.1 g / L with 10% inoculation amount, and cultured with shaking at 28 °C for 8 h; hydrogen peroxide was added to make the The final concentration of hydrogen peroxide was 2.5% by volume, and the culture was continued for 24 hours to obtain the culture solution; the culture solution was ...

Embodiment 3

[0053] A kind of rejuvenation of selenium-enriched yeast based on the activity of selenophosphate synthase The specific operation steps are as follows:

[0054] Step 1: Activation of the strain

[0055] Pick up a ring of selenium-enriched yeast strains to be rejuvenated with an inoculation needle and inoculate it on the slant of the solid medium, and activate it at 28 °C for 24 h; In a 250 mL shake flask, shake culture at 28°C for 15 h to obtain the activated bacterial liquid;

[0056] Step 2: Hydrogen peroxide (H 2 o 2 ) primary screening treatment

[0057] The activated bacterial liquid was taken and inoculated into 50 mL of liquid medium composed of wort powder 130 g / L and chloramphenicol 0.1 g / L with 10% inoculation amount, and cultured with shaking at 28 °C for 7 h; hydrogen peroxide was added to make the The final concentration of hydrogen peroxide was 2.3% by volume, and the culture was continued for 24 hours to obtain the culture solution; the culture solution was ...

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Abstract

The invention relates to a selenium-enriched yeast rejuvenation method based on activity of selenophosphate synthetase (SPS) and belongs to the technical field of yeast. The selenium-enriched yeast rejuvenation method comprises specific operation steps as follows: (1) activation of strains; (2) H2O2 primary screening treatment; and (3) secondary screening of selenium-enriched yeast with high SPS activity. The capacity of rejuvenated selenium-enriched yeast strains for enriching organic selenium is larger than 2000 [mu]g / g thalli by dry weight, and excellent strain guarantee is provided for fermentation production for converting inorganic selenium into organic selenium by the selenium-enriched yeast at a high level. Compared with an existing selenium-enriched yeast strain rejuvenation method, the selenium-enriched yeast rejuvenation method can directly screen out yeast strains for converting the inorganic selenium into the organic selenium at a high level with the activity of the SPS asa screening index, and the problems that existing rejuvenation methods cannot directly judge organic selenium enrichment capacity and cause rejuvenation failure easily in a rejuvenation process are solved; and by use of H2O2 as a selectivity factor, the workload is greatly reduced, and the uncertainty of organic selenium enrichment capacity of survival strains when the inorganic selenium is usedas the selectivity factor is avoided.

Description

Technical field [0001] The invention belongs to the technical field of yeast, and specifically relates to a rejuvenation method of selenium-rich yeast. Background technique [0002] Selenium is located in Group VI of the periodic table of elements. It is an essential trace element for the human body. It has biological activities such as antioxidant, improving immunity, and anti-aging. It plays an important role in the human central nervous system, male reproductive system, endocrine system, exercise system, and heart. It plays an important role in maintaining the homeostasis of the vascular system and other functions. Long-term selenium deficiency in the human body can easily lead to the occurrence of Keshan disease, Kashin-Beck disease, diabetes, cardiovascular and cerebrovascular diseases, neurodegenerative diseases and other diseases. Selenium exists in nature in two forms: inorganic selenium and organic selenium. Inorganic selenium is more toxic and can easily cause harm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12Q1/04G01N33/573
CPCC12N1/38C12Q1/04G01N33/573
Inventor 杨思林张玉英魏春厅
Owner 安徽省华信生物药业股份有限公司
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