A method for developing ssr primers for Chrysanthemum plants based on transcriptome sequencing
A technology of transcriptome sequencing and transcriptome sequence, which is applied in the direction of recombinant DNA technology, DNA / RNA fragments, etc., can solve the problems of small overall quantity and SSR primer reporting, so as to broaden the thinking and field, overcome the difficulty of obtaining, and increase the original data Effect
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[0089] The above method was used for high-throughput sequencing using chrysanthemum brain as a material. A total of 70,895 transcriptome sequences were generated, and 1,788 pairs of SSR primers were designed. The specific instructions are as follows:
[0090] Using the chrysanthemum brain of Chrysanthemum genus as material, the stems and leaves were collected for high-throughput sequencing of the transcriptome. A total of 70,895 transcriptome sequences were obtained by sequencing, and a total of 2,813 potential SSR sequences were detected after de-redundancy, accounting for 39.68% of the total. AC / TG content is the highest, followed by CA / GT and CCA / GGT ( figure 1 ). A total of 1788 pairs of SSR primers were designed using the primer3.0 batch design software.
[0091] 100 pairs of SSR primers were randomly selected from 1788 pairs of designed SSR primers, and the success rate of primer design was detected by using chrysanthemum brain DNA. The results showed that 81 pairs of S...
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