DNA (deoxyribonucleic acid) preserving solvent and DNA preserving method

Abstract

The invention discloses a DNA (deoxyribonucleic acid) preserving solvent and a DNA preserving method. The DNA preserving solvent comprises glycerol, TE buffer solution and 1-carboxyl-N, N, N-trimethyl-B lactone, wherein the final volume of the glycerol is 25-80%, the final concentration of the TE buffer solution is 1*TE-5*TE, and the final concentration of the 1-carboxyl-N, N, N-trimethyl-B lactone is 1-6mol / L. The DNA preserving method comprises the following steps of: (1) extracting DNA; and (2) dissolving the DNA in the DNA preserving solvent to be preserved. The DNA preserving solvent disclosed by the invention has the advantages of simple preparation, low cost, good preserving effect and long shelf life and is prevented from freezing and thawing repeatedly. The invention provides initial DNA records for genomics research, identity authentication, gene diagnosis, gene therapy, organ transplantation and the like in the future.

Description

technical field [0001] The present invention relates to a preservation of DNA (deoxyribonucleic acid, deoxyribonucleic acid), in particular to a DNA preservation solvent and a preservation method thereof. Background technique [0002] DNA is an important component of chromosomes and the basic genetic material of eukaryotes. It contains the genetic information of individual species and can be used to construct genomic libraries, isolate required genes, and detect related molecular markers. Because the amount of genetic information contained in DNA is large, easy to obtain, and has extremely stable chemical properties, it is one of the main contents of collection and preservation of germplasm resources and genetic resources. [0003] The sequence structure of DNA molecule has three important characteristics: (1) the sequence is different in different individuals; (2) it remains unchanged throughout life; (3) the DNA sequence in cells in different parts of the same human body i...

AI Technical Summary

Problems solved by technology

[0005] In order to maintain the integrity of the DNA structure and avoid large-scale DNA fragmentation and degradation, the best way to preserve DNA is to store it in dry powder at -80°C or at low temperature in liquid nitrogen. However, a large amount of DNA is required to make dry powder, and it is difficult to obtain materials. Not operational in resource collection

However, using absolute ethanol to preserve DNA, although it can be stored in advance, there is also the problem of repeated freezing and thawing.

The solid-phase DNA preservation technology is a new concept recently proposed, which is suitable for permanent preservation, and whether the preservation matrix of this method affects the subsequent DNA experiments and the specific effect of preservation, there is currently no data in this regard, and it remains to be tested by time

In addition, for samples that need to be used multiple times during storage, DNA needs to be re-extracted, which is complicated and can easily lead to DNA breakage, which will make it impossible to perform some detection experiments that require high DNA quality

Images