6 results about "Dna breakage" patented technology

The invention belongs to the technical field of biological medicines, and particularly discloses a bionic nano-vesicle for regulating and controlling neutrophil phenotype in combination with ultrasound, and a preparation method and application of the bionic nano-vesicle. The bionic nano-vesicle provided by the invention can be broken under the action of high-concentration glutathione existing in a tumor tissue microenvironment to release the sound-sensitive agent and functional siRNA, and under the action of US irradiation, the sound-sensitive agent is activated to generate a large amount of ROS, promote tumor cell DNA breakage, generate a large amount of double-stranded DNA, activate APC and promote IFN-beta release, so that the tumor tissue microenvironment is inhibited. The released functional siRNA can promote the N2-type neutrophile granulocyte to become N1-type neutrophile granulocyte, and the released functional siRNA can prevent the N1-type neutrophile granulocyte from being reversed to the N2-type neutrophile granulocyte again, so that the regulation and control on the phenotype of the neutrophile granulocyte are realized; under catalysis of CAT rich in the thylakoid membrane, H2O2 overexpressed by tumor tissue is decomposed into oxygen, the tumor hypoxia microenvironment can be relieved, and the tumor treatment effect can be improved.

The invention discloses a method for improving in-vitro maturation quality and cryopreservation effect of porcine oocytes, and relates to the technical field of biology. By adding the NMN, the comprehensive efficiency of in-vitro maturation and cryopreservation of the porcine oocytes is remarkably improved. Research finds that by adding NMN into an IVM culture medium, maturation of cell nucleuses is promoted, discharge of first polar bodies of oocytes is improved, oxidative stress and lipid peroxidation of the oocytes are reduced, mitochondrial activity is improved, and cytoplasm maturation of the oocytes is facilitated. Meanwhile, after the NMN is used for pretreatment, the survival rate of the vitrified and frozen oocytes is remarkably improved, and the active oxygen level and the DNA breakage level are reduced. Therefore, the addition of the NMN in the IVM culture solution may become a new method for improving and optimizing the IVM technology, and an innovative solution is provided for pig germplasm resource preservation and excellent breeding stock breeding.

The application discloses a preparation of a biological memristor and application of the biological memristor in sperm DNA breakage detection, and takes supernatant of a sample to be detected, and drops the supernatant on the surface of the memristor; after the memristor is connected with a detection platform, different voltage corresponding electrical signals are outputted, and the normal sperm and the sperm with DNA breakage are distinguished. The application has the characteristics of simple detection process, low cost, high sensitivity and fast response, and can quickly distinguish whether sperm DNA sequence breakage exists in sperm cells of a male patient with infertility.

Embodiments provide a construction method for a high-throughput sequencing library, a kit, and a use. The method comprises: using a Tn5 transposase complex to randomly break a DNA in a genome sample to obtain a DNA breakage product; carrying out molecular hybridization on an RNA enrichment probe and the DNA breakage product, and using streptavidin magnetic beads to collect an RNA-DNA complex in a molecular hybridization product; and carrying out methylation treatment and PCR amplification on the RNA-DNA complex to obtain a sequencing library. The method achieves directed enrichment of a target methylation site in the genome sample; when a new target methylation site needs to be added, an RNA enrichment probe is simply designed for same; when the target methylation site that has been proven to have a poor effect needs to be deleted, the RNA enrichment probe corresponding thereto is simply removed; and by means of the directed enrichment of the target methylation site, the concentration requirement of the genome sample is reduced, and trace detection is facilitated.

The present invention discloses the use of Gardenia jasminoides-derived extracellular vesicles in the preparation of drugs for preventing and treating neurodegenerative diseases, and relates to the field of biomedicine. The present invention applies Gardenia jasminoides-derived extracellular vesicles to anti-oxidation and neuroprotection. After being ingested, Gardenia jasminoides-derived extracellular vesicles can significantly improve the decreased viability of PC12 cells induced by rotenone, and effectively reduce the levels of reactive oxygen species (ROS) and superoxide anions (O2 ‑ ) production, thereby alleviating oxidative stress. Furthermore, treatment with gardenia fruit-derived extracellular vesicles significantly reduced DNA breakage and cell membrane damage in neurons caused by rotenone treatment, demonstrating a significant protective effect on the nuclei and cell membranes of neurons. Therefore, the gardenia fruit-derived extracellular vesicles described in the present invention possess significant antioxidant and neuroprotective potential.

The invention relates to the technical field of biological medicine, in particular to a synthetic method and application of a benzimidazole borneol derivative, and the synthetic method comprises the following steps: by taking a benzimidazole compound, borneol, phenylboronic acid with different substitutions and acetophenone as raw materials, carrying out a substitution reaction, a Suzuki coupling reaction, a condensation reaction and other multi-step reactions to obtain 21 target compounds; the structure of the compound is characterized by 1H NMR, 13C NMR and HRMS, and glioblastoma U87 and U251 cells are used as research objects. The benzimidazole disclosed by the invention can play an anti-cancer role in various modes, and the structure similar to purine can effectively enhance the capabilities of inhibition on protein and nucleic acid, microtubule degeneration, cell apoptosis, DNA breakage, electrostatic interaction between hydrogen bonds and topoisomerase complexes, embedding with DNA and the like. Meanwhile, natural bicyclic terpene borneol has high fat solubility and has a certain promoting effect on permeability of BBB, the bicyclic terpene borneol is widely applied to an auxiliary agent of central drugs so as to improve drug delivery to the brain, and the bicyclic terpene borneol is novel in product structure and high in yield.