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Methods and systems for reducing DNA fragmentation in a population of sperm cells

A cell population and sperm technology, applied in animal cells, germ cells, vertebrate cells, etc., can solve problems such as abnormal embryonic genes

Inactive Publication Date: 2012-09-12
INGURAN LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Damaged sperm, when present in viable sperm populations used in assisted reproductive technologies, may be able to fertilize eggs, but may fail to produce viable embryos or produce embryos with genetic abnormalities (which will not function normally develop or die later)

Method used

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  • Methods and systems for reducing DNA fragmentation in a population of sperm cells
  • Methods and systems for reducing DNA fragmentation in a population of sperm cells
  • Methods and systems for reducing DNA fragmentation in a population of sperm cells

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Embodiment Construction

[0020] It is easy to understand that before explaining the method in detail, this is not a specific limitation to the implementation method described herein, which can be implemented in various ways. Furthermore, the methods and systems are disclosed in a general manner so that once one understands the general principles, the methods can be applied to specific systems.

[0021] One embodiment relates to a method of sorting cells into discrete subpopulations, wherein some enriched subpopulations have reduced or no DNA fragmentation levels and other enriched subpopulations have increased DNA fragmentation levels. This sorting is based on the presence of DNA fragmentation signatures to reduce the incidence of DNA fragmentation in enriched sperm samples. The method begins by establishing a sperm sample. The sperm sample may be obtained from any mammal, without limitation, including those listed in Wilson, D.E. and Reeder, D.M., Mammal Species of the World, Smithsonian Institute P...

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Abstract

The invention relates to a method and a system for sorting sperm samples according to different levels of DNA fragmentation and methods of using populations with low levels of DNA fragmentation to improve fertility and success rates of assisted reproductive procedures, including artificial insemination, in vitro fertilization, intracytoplasmic injection, and other related techniques.

Description

technical field [0001] Embodiments of the present invention generally relate to methods and systems for reducing DNA fragmentation in a sperm population, and in particular, to forming a sperm population with reduced DNA fragmentation for use in assisted reproductive processes to produce offspring. Background technique [0002] Sperm and sex-sorted sperm (sperm are sorted based on carrying an X or Y chromosome) are biomaterials of great interest in assisted reproductive technology and animal husbandry. However, damaged and / or dead sperm lack the ability to produce offspring by artificial insemination (AI), in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) or other assisted reproductive techniques. Damaged cells can include cells with altered membranes, cells undergoing apoptosis, and cells with DNA fragmentation. Damaged sperm, when present in viable sperm populations used in assisted reproductive technologies, may be able to fertilize eggs, but may fail ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076C12Q1/68C12M1/34
CPCC12N5/0612G01N21/6428G01N21/645
Inventor J·莫雷诺M·埃文斯M·杰兰德J·格萨维茨C·洛佩兹·费尔南德斯
Owner INGURAN LLC
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