1results about How to "Specific binding" patented technology

This invention belongs to the field of bioengineering technology, specifically relating to the preparation and application of colloidal gold immunochromatographic assay based on AMH nanobodies. This invention discloses two AMH nanobodies, the amino acid sequences of which are shown in SEQ ID NO.1 and SEQ ID NO.2, and the nucleotide molecules encoding these amino acid sequences are shown in SEQ ID NO.3 and SEQ ID NO.4. This invention also discloses a method for preparing colloidal gold immunochromatographic assay based on AMH nanobodies. The results of the examples show that the molecular weight of both AMH nanobodies is approximately 15 kDa, consistent with the size of nanobodies. The purified nanobodies can be used for the preparation of colloidal gold immunochromatographic assay, and the colloidal gold can be used for the detection of AMH in the serum of sheep, donkeys, horses, and camels.