Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapid detection of in vitro DNA break damage strength by plasma

A plasma and intensity technology, applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of complex operation, inaccurate measurement of ARTP dose, lack of rapid detection method for DNA breakage damage intensity, etc., and achieve a wide range of applications Effect

Active Publication Date: 2017-04-05
WUXI TMAXTREE BIOTECHNOLOGY CO LTD +1
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the operating parameters of the ARTP mutagenesis system, power is usually used as a parameter, and power (P in )20-120W, gas flow (Q He )5-20slpm, irradiation distance (D) is 2-5mm, but the dose of ARTP has not been accurately measured under different operating conditions
[0007] The R&D team has qualitatively verified the damage of ARTP to DNA by means of gel electrophoresis, but so far, there is a lack of rapid detection methods for the intensity of DNA damage, which can then be used to characterize the dose of ARTP
Comet method (single-cell gel electrophoresis) is a semi-quantitative method for detecting DNA breakage damage (Kumari S, Rastogi R P, Singh K L, et al. DNA damage: Detection strategies. Excli Journal, 2008, 7:44-62. ), but its operation is complicated and can only be used for large DNA fragments

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapid detection of in vitro DNA break damage strength by plasma
  • Method for rapid detection of in vitro DNA break damage strength by plasma
  • Method for rapid detection of in vitro DNA break damage strength by plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The method of the present invention will be described by taking Atmospheric and Room Temperature Plasma (ARTP) in the plasma as an example.

[0035] figure 1 It is a schematic diagram for determining the damage strength of ARTP on DNA fragmentation in vitro. Firstly, ARTP is used to treat DNA-coated microspheres, and the active particles produced by ARTP react with the DNA on the microspheres through reaction or energy transfer, and randomly break the DNA chains on the microspheres. DNA fragments with different lengths after being broken from different sites are detached from the microspheres and free in the aqueous solution outside the microspheres. The degraded DNA fragments can be separated from the treated DNA microspheres by centrifugation. SYBR Green is used to stain the treated DNA-coated microspheres, and the amount of remaining DNA on the microspheres can be determined by fluorescence spectrophotometry. Compared with the amount of DNA on the microspheres be...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
emission peakaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for rapid detection of in vitro DNA break damage strength by plasma, and the method is used for determining the damage strength of plasma mutagenesis methods including atmospheric and room temperature plasma (ARTP) on DNA. The method includes the steps of: 1) treating DNA coated microspheres by plasma mutagenesis method, and setting a control group without plasma treatment; 2) performing washing, and then dyeing the DNA coated microspheres with a dye; 3) determining the fluorescence value of the dyed DNA coated microspheres by a fluorescence detector; and 4) calculating the DNA relative content of the control group and the plasma mutagenesis treated microspheres according to the fluorescence value, and judging the gene damage strength of plasma. The invention utilizes DNA coated microspheres to establish a rapid detection method for in vitro DNA damage strength so as to realize direct and effective evaluation of plasma caused damage strength to DNA.

Description

technical field [0001] The invention relates to a rapid detection method for DNA damage intensity in vitro caused by plasma, in particular to establishing a rapid detection method for DNA damage intensity in vitro by using DNA-coated microspheres. Background technique [0002] High-quality microbial strains are always the core of the biological industry. How to use efficient biological mutagenesis technology to achieve rapid optimization of strains is an important task for the biological industry. [0003] Increase the mutation rate by physical (such as X-ray, γ-ray, UV, ion beam implantation, plasma), chemical (such as alkylating agent, azide), biological (such as expression of mutS, ndk gene) and other means, which can be used in Create more genotypes in a short time. Different mutagenesis methods cause DNA damage in different ways. For example, ultraviolet (UV) mainly causes cyclobutane pyrimidine dimer (CPD) and 6-4 photoproducts (6-4PPs), thereby causing mutations from...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 邢新会张翀张雪王立言
Owner WUXI TMAXTREE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com