8 results about "Dna breaks" patented technology

Cancer poses a significant threat to human health, and currently there is no particularly effective treatment. The primary reason is the lack of a highly effective method to specifically kill cancer cells while minimizing impact on normal cells. DNA breaks, if left unrepaired, lead to cell death—this is the fundamental principle of radiotherapy—but radiotherapy also causes DNA damage in normal cells. This invention proposes a method for specifically killing cancer cells. It utilizes gene editing technology to create DNA breaks unique to cancer cells (these DNA cut points are absent in normal cells), simultaneously combining this with DNA damage repair inhibitors to suppress DNA repair in cancer cells, leading to specific cancer cell death. This invention provides a new approach to precision cancer treatment.

The application discloses a dCas9-based c-NHEJ site-directed inhibition system and application in improving HR-mediated gene editing efficiency, and the system comprises the following elements: (1) element 1: a nuclease for site-directed induction of DSB in eukaryotic cells and accompanying elements thereof; (2) element 2: dCas9-sgRNA for binding to the end of DNA break; (3) element 3: a homologous template for HR-mediated gene editing. The system synergistically improves gene editing efficiency. The application provides site-directed and efficient inhibition of the c-NHEJ repair pathway, site-directed and efficient improvement of the HR repair efficiency, effective improvement of the HR-mediated CRISPR gene editing efficiency, and reduction of off-target effects.

This specification discloses the use of alternating electric fields and DNA-dependent PK inhibitors for cancer treatment. This specification discloses the use of alternating electric fields and DNA-dependent PK inhibitors for inducing cell death. This specification discloses the use of alternating electric fields and DNA-dependent PK inhibitors to inhibit DNA repair in cancer cells having DNA strand breaks (e.g., double-strand DNA breaks). This specification discloses the use of alternating electric fields and DNA-dependent PK inhibitors to enhance the effectiveness of radiotherapy.

The present application belongs to the technical field of biological medicine, and particularly relates to a kind of targeting REXO4 liquid-liquid phase separation interference polypeptide and its application.The interference polypeptide of the present application includes targeting peptide, the targeting peptide is specifically combined with REXO4 protein liquid-liquid phase separation driving domain, and the liquid-liquid phase separation of REXO4 protein is interfered;Wherein, the REXO4 protein liquid-liquid phase separation driving domain is REXO4 protein N-terminal disordered domain, and the amino acid sequence of the REXO4 protein N-terminal disordered domain is shown as sequence 1.The interference polypeptide of the present application can be specifically combined with REXO4 protein liquid-liquid phase separation driving domain, interfere with and destroy the functional LLPS of REXO4, cause the DNA damage repair efficiency in tumor cell to be significantly reduced, lead to the DNA break caused by ionizing radiation cannot be repaired in time, finally enhance the sensitivity of tumor cell to ionizing radiation, realize the effect of radiotherapy sensitization.

The utility model provides a bottom support for a DNA (Deoxyribose Nucleic Acid) breaking instrument, which belongs to the technical field of DNA breaking instruments, comprises a motor kinetic energy device for driving a test tube for containing a DNA sample to rotate, and is characterized by further comprising a platform plate for supporting and a DNA mounting module for normatively placing the test tube, a central through hole is formed in the middle of the platform plate, and the DNA installation module is placed and installed in the central through hole; the platform plate is further provided with a notch used for containing the motor kinetic energy device. A driving lever for driving the DNA mounting module to rotate is fixedly mounted on an output shaft of the motor kinetic energy device, and contacts are arranged at the two ends of the bottom of the driving lever; the bottom platform of the DNA breaking instrument can solve the problems that when an existing DNA breaking instrument is used, a tray for containing DNA sample test tubes shakes, and the whole tray is difficult to take and place.

B cells with an altered function. FIG. 1A Rearrangement at the Ig locus n - 250-1,000) VH1 VHP DH1-12 T FEu DNA-PKcs RAG1 / 2 VIDN Excised Ku70, Ku80 NHEJ recombination intervening DNA ARTEMIS XROC4 DNA Ligase N V DJ Cp C6 OB Cr1 0120 area Ce Cex 3' RR Eu Su Se At S regions: DNA breaks AD Class-switch DNA repair recombination IgM V D JCu Transcription Recombined CH locus V DJ Ce Ca 3' RR Cy2a Cu Eµ C8 Excised Cy2b intervening DNA IgE V DJCe Cy3 Cyl 20 26 20 47 61 19 J un 2 02 6 A B S T R A C T 1 9 J u n 2 0 2 6 2 0 2 6 2 0 4 7 6 1 F I G . 1 A R e a r r a n g e m e n t a t t h e I g l o c u s V H 1 V H n D H 1 - 1 2 7 F E u r D N A - P K c s R A G 1 / 2 V I D N N H E J V D J C p C 6 O B 0 1 2 0 O R D C e E u S u S e A D r e c o m b i n a t i o n I g M V D J C u R e c o m b i n e d V D J C e C a 3 ' R R C y 2 a C u E u r C 8 E x c i s e d C y 2 b I g E V D J C e C y 3 C y l

The invention relates to the technical field of genetic engineering and bioinformatics, in particular to a method for creating a new gene in a living body on the premise of no artificial DNA template and application. The method is characterized in that DNA fractures are simultaneously generated at at least two different specific positions in the genome of an organism, the specific positions are genomic sites capable of segmenting different gene elements or different protein structural domains, and the DNA fractures are connected with one another by means of non-homologous end connection (NHEJ) or homologous repair. And generating a new combination of the different gene elements or different protein structural domains, which is different from the original genome sequence, so as to form a new gene. The new gene provided by the invention can change the growth, development, resistance, yield and other characters of organisms, and has important application value.

In some aspects, this disclosure provides a high throughput method of identifying highly active and / or highly specific guide RNAs.