5 results about "Mitotic cell" patented technology

A method for automatic mitosis detection based on data and feature diversity is provided, in which a training-free hematoxylin-based detection approach is employed to obtain candidate samples; a balanced sampling strategy is applied to remove redundant information from the candidate samples, balance the data volume, and maintain sample diversity and eliminate easy samples, so as to obtain balanced and representative training dataset that facilitate a classifier in learning representative features; in view of the morphological complexity of mitotic cells, a jointly trained classifier is designed, in which subclass division is performed based on binary classification, the divided subclasses are used as pseudo-labels, and the classifier is trained with parent-class labels and subclass pseudo-labels to obtain a parent-subclass joint classifier. A system for implementing such method is also provided.

The present disclosure provides methods for inducing cellular quiescence—defined as a reversible arrest of cell proliferation—by inhibiting ribosome biogenesis. The methods involve administering a quiescence-inducing agent, such as a small molecule inhibitor, peptide, antisense oligonucleotide, or targeted degrader, that interferes with rRNA transcription, processing, ribosomal protein synthesis, or ribosome assembly. The agent may be applied to cultured cells, tissues, organs, or organoids, across a range of cell types and species. In some embodiments, the methods reduce metabolic activity and enhance stress resistance in non-dividing or post-mitotic cells. By slowing proliferation and / or metabolism while preserving viability, these methods enable improved preservation of cells, tissues, and organs-enhancing transplantation logistics, biobanking, regenerative medicine workflows, and biological transport. Additional applications include studies of cell cycle regulation, tissue regeneration, stress biology, aging, and induction of organismal hypometabolism (e.g., torpor or hibernation). The disclosure includes examples demonstrating quiescence induction using chemical biology systems, pharmacological agents, and genetic approaches.

This invention provides a method for enriching mitotic cells that allows for efficient collection of mitotic cells and better observation of the properties of cell aggregates, as well as enabling the use of such enriched cells. [Solution] A method for enriching mitotic cells is used, which involves first dispersing densely cultured cells, and then enriching the cells while culturing them in suspension to increase the proportion of mitotic cells.

Provided herein is a method of treating epileptic seizure activity by administering to a subject in need thereof a therapeutically effective amount of a cell composition comprising pluripotent stem cell-derived, MGE-type inhibitory (e.g., GABAergic) neuronal cells. In some embodiments, at least 90% of the cells in the composition are post-mitotic cells. In some embodiments, at least 50% of the cells in the composition express LHX6, ERBB4, and GAD1.

A B S T R A C T The invention relates to a combination therapy comprising administering a mitotic- phase (M-phase) cell cycle arresting compound and a modulator of an interphase cell cycle checkpoint to a subject and delivering ionizing radiation to the same subject for the treatment of a tumor. Provided is for (M-phase) cell cycle arresting compounds, modulators of an interphase cell cycle checkpoint and ionizing radiation for use in such treatments, as well as methods of the treatment involving the use of (M-phase) cell cycle arresting compounds and modulators of an interphase cell cycle checkpoint and ionizing radiation.