41 results about "Hematoxylin stain" patented technology

The invention provides a hematoxylin staining solution which comprises the following components of hematoxylin, aluminium salt, an oxidizing agent, benzalkonium chloride, alcohol, weak acid and water. The invention further provides a preparation method of the hematoxylin staining solution, a HE (hematoxylin eosin) staining solution containing the hematoxylin staining solution, and a staining method. When the hematoxylin staining solution is used to stain a section, the background of the section is clear, the section is well arranged, the staining effect is excellent, the section is easy to read, the color of the section does not fade easily, and the section can be stored conveniently for a long period of time.

The invention provides a mercury-free environment-friendly hematoxylin staining solution, which comprises the following components in portions by volume: 100-300 portions of 3-10 percent hematoxylin absolute ethyl alcohol solution, 1500-2000 portions of 2-6 percent aluminum sulfate aqueous solution, 60-100 portions of .05-2 percent periodic acid aqueous solution, and 15-30 portions of glacial acetic acid. Non-toxic and innocuous chemical reagents are adopted for the mercury-free environment-friendly hematoxylin staining solution, and a histological section staining experiment shows that the nucleus is clearly stained and the structures of the chromatin, nuclear membrane and nucleolus are clear.

The invention discloses a papanicolaou staining kit and a staining method thereof. The kit comprises hematoxylin stain, a back-to-blue liquid, eosin stain and a cleaning solution. The eosin stain is prepared from the following substances in concentration: 600-900ml/L ethanol, 100-400ml/L methanol, 1-4g/L eosin Y, 1-4g/L phosphotungstic acid, 0.01-2g/L fast green and 1-10ml/L weak acid; and the cleaning solution is prepared by mixing solvent oil, absolute ethyl alcohol and propylene glycol methyl ether acetate according to a volume ratio of (10-350):(600-950):(10-200). The papanicolaou stainingkit disclosed by the invention is rapid in staining, the stained colors have clear differences, and the diagnosis efficiency is effectively improved.

The invention discloses a kit and a staining method for staining mycobacterium tuberculosis and the expression protein of the mycobacterium tuberculosis. The method for staining the mycobacterium tuberculosis and the expression protein of the mycobacterium tuberculosis comprises the following steps: (1) carrying out primary antibody incubation and secondary antibody incubation to a to-be-tested sample containing mycobacterium tuberculosis to obtain an antibody binding product; and (2) carrying out DAB (diaminobenzidine) staining solution staining, carbolfuchsin staining solution staining and hematoxylin staining solution staining to the antibody binding product sequentially, so as to obtain stained mycobacterium tuberculosis and the stained expression protein. Experimental results show that a novel kit for the tuberculotic pathology diagnosis is developed, and the kit can detect the mycobacterium tuberculosis and the expression protein in one tissue specimen section simultaneously, so as to improve the tuberculotic pathological diagnosis sensibility. The kit can be used conveniently and simply, is suitable for the conventional detection for the pathology department and has good clinical promotion value.

The invention relates to Papanicolaou staining fluid and a using method thereof. The Papanicolaou staining fluid consists of fluid A, fluid B and 95 percent ethanol, wherein the fluid A is improved hematoxylin staining fluid; the fluid B is prepared from a phosphotungstic acid, eostm and light green. A method for preparing the improved hematoxylin staining fluid comprises the following steps of dissolving hematoxylin into the 95 percent ethanol, dissolving potassium alum into 100 DEG C boiling water, mixing two mixed solutions, adding an oxidant, stirring for at least 50 minutes, adding a glacial acetic acid, and stirring for at least 50 minutes. A method for preparing the fluid B comprises the following steps of mixing 95 to 97 mass percent of 0.2 to 0.6 percent phosphotungstic acid solution, 1.5 to 2.5 mass percent of eostm solution and 1.5 to 2.5 mass percent of light green solution. When the Papanicolaou staining fluid is used, a smear is fixed in the 95 percent ethanol, and is flushed in a specific environment by adding a corresponding amount of fluid A and a corresponding amount of fluid B. The Papanicolaou staining fluid has the advantages of low cost, bright color, clear contrast, coloring stability, is simple to prepare and convenient to operate, is easily popularized and used in various medical units and the like, and is applied to the display and diagnosis of the estrogen level of a vaginal smear and the poorly-differentiated small-angle cell carcinoma of a cervical smear and a sputum smear.

The invention discloses a hematoxylin-eosin rapid staining solution and an application method thereof, which belong to the technical field of biology. Specifically speaking, the hematoxylin-eosin rapid staining solution is prepared from an improved hematoxylin staining solution and a novel eosin staining solution. The hematoxylin-eosin rapid staining solution provided by the invention is easy to operate, staining is uniform, a stained cellular structure is clear, cell coloration is vivid, and clinical pathological diagnosis, observation and judgment are facilitated.

The invention discloses an improved HE staining method. The method includes steps of dewaxing, rehydration and hematoxylin staining of sections under electromagnetic stirring at a temperature of 41-44DEG C. The method enables the dewaxing, rehydration and hematoxylin staining steps to be carried out at an elevated temperature and cooperates with the electromagnetic stirring, and so the staining speed is greatly accelerated. Furthermore, the stirring and temperature conditions in the steps are the same, and the steps can be sequentially carried out in the same constant temperature equipment, which is convenient for the operation and is more convenient for the staining quality control and the consistent staining effect.

An image processing device for processing a stained sample image including hematoxylin stain is provided with a dye spectrum storage unit that stores a dye spectrum of dye used in staining, a change characteristic calculation unit that calculates a change characteristic in a wavelength direction of the dye spectrum based on the dye spectrum, a dye amount/wavelength shift amount estimation unit that estimates at least a dye amount of the hematoxylin stain and a shift amount in the wavelength direction for each pixel in the stained sample image based on the dye spectrum and the change characteristic; and a cell nucleus extraction unit that extracts a cell nucleus region of the stained sample image based on the shift amount estimated in the wavelength direction.

The invention relates to a pathological tissue section staining kit, which belongs to the field of pathological tissue section staining and comprises a section dewaxing solution, a conversion solution, a cleaning solution, a differentiation solution, a blue returning solution, a hematoxylin staining solution, an eosin staining solution and a mounting medium, the invention provides the novel pathological tissue section staining kit which is simple in component, good in effect and low in toxicity, and the preparation method, and the kit is mainly used for pathological tissue H&E staining. The method is characterized in that 1, the harm of traditional dyeing reagents such as dimethylbenzene and alcohol to an operator is reduced; 2, the pollution to the environment is reduced; 3, extra deionized water does not need to be introduced in the dyeing process; 4, a differentiation solution and a blue returning solution are designed by adopting a buffer system, so that the effect is milder, and the coloring effect of H&E dyeing is clearer and brighter; and 5, compared with a traditional hematoxylin formula, beta-cyclodextrin is introduced into the kit to neutralize a product iodine reduced by sodium iodate, so that the dyeing capability of the staining solution is improved, and meanwhile, a reducing agent dibutylated hydroxytoluene is introduced, so that the service life and the shelf life of the staining solution are prolonged.

The invention relates to the field of biomedical reagents and in particular relates to a hematoxylin staining solution and a preparation method. The formula usage amount is reasonable, and the staining effect is ideal. The tissue slice stained by the hematoxylin staining solution provided by the invention is excellent in cell nucleus staining and clear in structure, and the tissue slice stained byeosin is bright in color, strong in contrast and easy to observe, and the staining effect is ideal. The staining method of the hematoxylin staining solution provided by the invention is simple and easy to operate, heating is not needed in the preparation process, the staining process is safe, and the tissue slices are not contaminated.

The invention discloses a semi-supervised learning method special for performing cell nucleus segmentation on a histopathological image dyed by hematoxylin eosin. According to the cell nucleus segmentation method provided by the invention, according to the characteristics of the histopathological image and cell nucleus segmentation, the two dyes of hematoxylin and eosin in the histopathological image are separated by adopting non-negative matrix factorization with sparse constraint, and then the eosin dye in the histopathological image is replaced by the eosin dye in other histopathological images, so that the segmentation efficiency of the cell nucleus is improved. Therefore, a group of positive example samples can be prepared, and the positive example samples have the same hematoxylin staining agent, so that the positive example samples have interpretable invariance. And inputting the multiple groups of positive example samples into an encoder, and outputting a corresponding embedded representation vector by the encoder. And constraining the model by adopting a contrast learning loss function, so that the model can learn invariance in a positive example sample, namely the hematoxylin staining agent. The hematoxylin stain can stain the cell nucleus and other nucleic acid-rich parts, such as ribosome, so that the hematoxylin stain and the cell nucleus have relatively high correlation. When the model learns the characteristics of the hematoxylin stain, the characteristics accord with the characteristics of a cell nucleus segmentation task, so that the training of the downstream cell nucleus segmentation task is facilitated. As positive example sample construction and pre-training do not need labels, a large amount of unlabeled data can be utilized for training in the mode. And finally, the pre-trained encoder is added into the segmentation model, and fine adjustment is performed on a very small amount of labeled data, so that an effect better than supervised learning on a small amount of samples can be achieved. Therefore, the demand of annotation data is also reduced, and the labor cost is greatly reduced.

This disclosure relates to hematoxylin staining formulations and particularly to formulations for use in autostainers. The disclosed compositions were discovered to possess atypical stability under storage while having high stain quality and sufficiently fast staining performance. The disclosed hematoxylin staining compositions include a solvent system, hematoxylin, a chemical oxidant, and a mordant. Illustrative embodiments also have a Cl-/SO4- molar ratio of between about 2.5/1 and about 1/4.

An intraoperative quick immunohistochemical frozen-section staining method comprises the following steps: placing an intraoperative fresh specimen in a freezing microtome for freezing and then slicing, wherein the section thickness is 5 microns, pasting a section on a glass slide, and carrying out air drying for 25-35 seconds; dropwise adding a primary antibody for incubation at 40 DEG C for 4 minutes; executing washing with PBS three times for 4-7 seconds each time; dropwise adding a secondary antibody for incubation at 40 DEG C for 4 minutes; executing washing with PBS three times for 4-7 seconds each time; dropwise adding a DAB reagent, and executing development for 1-2 minutes; executing staining with hematoxylin stain for 3-10 seconds. The staining process is not fixed, endogenous blocking is avoided, the incubation temperature is increased, the reaction time is shortened, the steps are omitted, the staining time is shortened, the total experiment time is greatly shortened, the whole process time needs about 12 minutes, the possibility of assisting the rapid frozen pathology diagnosis time is provided, the frozen diagnosis accuracy is improved, and the method has good repeatability and comparability with immunohistochemical results of paraffin.

The invention relates to the technical field of tissue cell staining, and discloses an HE staining method. The HE staining method comprises the steps of dehydrating, degreasing and enriching a biological material, carrying out hematoxylin staining, cleaning, bluing, carrying out eosin staining to complete HE staining, and dehydrating the biological material by using ethanol solutions with different concentrations, thereby enhancing the dehydration efficiency. Bluing can enable cell nucleus to present obvious blue. The eosin dye liquor can dye the cytoplasm red, so that the comparison betweenthe cytoplasm and the cell nucleus is more distinct, and the HE staining effect is improved. The invention further discloses an HE staining device which comprises a sample preparation assembly, a plurality of solution bottles and a peristaltic pump, a solution is added into or pumped out of the sample preparation assembly through the peristaltic pump, so that the working efficiency is improved; and the sample preparation assembly comprises a test tube, a water bath kettle and an ultrasonic transducer, and can realize water bath heating and uniform oscillation.

The invention discloses a cervix uteri abnormal cell recognition method and device and electronic equipment. The method comprises the steps that a cervix uteri cell section image is acquired; the cervical cell slice image is input into a pre-trained channel separation module to obtain an H image and a DAB image, with the H image being a hematoxylin stained image, and the DAB image being an immunohistochemical stained image; and abnormal cervical cells are identified by using the H image and the DAB image. According to the technical scheme, the cell detection speed can be obviously increased in cervical cancer positive cell detection, the recognition accuracy is enhanced, and the algorithm execution efficiency is improved.

The invention discloses a hematoxylin staining solution. The hematoxylin staining solution comprises the following components: 90ml of isopropanol, 20ml of methanol, 50-80ml of alcohol, 13ml of aceticacid, 37g of aluminum sulfate, 3.5g of hematoxylin, 0.5g of sodium iodate, 7g of sodium chloride, 3g of disodium hydrogen phosphate, 0.4g of sodium azide, 0.3g of dodecyl dimethyl benzyl ammonium bromide, and 750-850ml of water. The invention further provides a preparation method of the hematoxylin staining solution. The preparation method of the hematoxylin staining solution is simple, a staining process is safe, staining operation is simple and convenient, and the staining solution achieves high staining capability and a long preservation cycle.

The invention relates to a pathological section staining composition of ovarian cancer. The composition is prepared from hematoxylin, absolute ethyl alcohol, aluminium potassium sulfate, sodium iodate, glacial acetic acid, ferrous sulfate, ferrous sulfate chitosan PLGA double microspheres and distilled water. According to the composition, the components of the hematoxylin staining fluid are simple, the cost is low, the staining fluid is safe and effective and has no toxic and side effects on the human body, in the preparation process, there are no strict requirements on the dosage of oxidizingagent and the oxidation time, the difficulty of preparation is effectively reduced, the consumed time for preparation is obviously shortened, after preparation of the staining fluid is completed, thestaining ability can be maintained for a long time, no oxidation film and precipitation can be formed, the validity period of the hematoxylin staining fluid is greatly prolonged, the staining composition has an excellent staining ability for various pathological tissues, especially, the staining effect on the ovarian cancer is better, and nuclei, chromatin, nuclear membranes and nucleoli can be stained clearly.

The invention discloses a mercury-free hematoxylin dyeing solution, which consists of a mixed solution and added components, wherein the mixed solution consists of the following components by volume: 1000-1500 parts of distilled water, 70-100 parts of glycerin, and 40-60 parts of 1-2% periodic acid aqueous solution, the added components include the following components, and the final concentrations of each component in the dyeing solution are: hematoxylin 6-8 g/L, aluminum potassium sulfate 40 ~60g/L and citric acid 3~6g/L. The mercury-free hematoxylin dyeing solution of the invention has short dyeing time and good dyeing effect. The stained cells showed clear nuclear staining and clear chromatin, nuclear membrane and nucleolar structures.

The invention discloses a hematoxylin staining liquid for immumohistochemical staining and a staining method. The hematoxylin staining liquid is a Mayer's hematoxylin staining liquid, wherein the pH value of the hematoxylin staining liquid is 2.4-2.8. The hematoxylin staining liquid disclosed by the invention has a staining effect of a high color contrast ratio after an antigen retrieval step.

The invention relates to a hematoxylin staining solution oxidation degree monitoring method and a hematoxylin staining solution quality control method, and belongs to the technical field of hematoxylin staining solutions. The hematoxylin staining solution oxidation degree monitoring method comprises the following steps: (1) detecting an OD value of a diluent of the hematoxylin staining solution before oxidation by using an ultraviolet spectrophotometer, and recording the OD value as OD0; (2) detecting the OD value of the diluent of the oxidized hematoxylin staining solution by using an ultraviolet spectrophotometer, and recording the OD value as ODt; (3) before and after oxidation, observing that the OD value of the diluent of the hematoxylin staining solution changes regularly, and the change rule is as follows: the larger the oxidation degree is, the larger the numerical value of the OD value is; monitoring the oxidation degree of the hematoxylin staining solution according to the increase degree of the ODt relative to the OD0. The quality control method is rapid, accurate and easy to popularize and use. The method solves the problem that the quality of dyed tablets is always baddue to the fact that the quality of the existing hematoxylin staining solution cannot be controlled.

The invention relates to an improved hematoxylin staining solution and a preparation method thereof and belongs to the technical field of biology. The improved hematoxylin staining solution is used asa nucleus staining solution of a Papanicolaou staining solution and is composed of the following components: hematoxylin (C16H14O6), absolute ethyl alcohol (C2H6O), aluminum potassium sulfate (KAl(SO4)2) or aluminum ammonium sulfate (NH4Al(SO4)2) or a mixture of the two compounds, distilled water (H2O), mercury oxide (HgO) and glacial acetic acid (C2H4O2). The improved hematoxylin staining solution provided by the invention can be used as the nucleus staining solution of the Papanicolaou staining solution for observing and judging pathological sections in clinic; the cell nucleuses of cells to be detected are dark blue or dark purple; the nucleus cores are red; the staining results can be observed in multiple directions in clinic; the pathologic conditions of the sections can be judged, so that the judgment results are more accurate.