H-E staining solution for liquid-based cytology experiments

A staining solution and cytology technology, applied in the field of H-E staining solution, can solve the problems of complex preparation of H-E staining solution, affect the accuracy of pathological reading, and unstable staining effect, and achieve clear cell structure, bright cell coloring, and easy promotion Effect

Inactive Publication Date: 2019-03-05
武汉宏强医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The problem to be solved by the present invention is that the preparation of the traditional H-E staining solution is relatively complicated, the staining solution is easy to be old, and the staining effect is unstable, and the effect is not good when it is used with an automatic liquid-based cell film maker with a high degree of automation, which affects the doctor's pathological reading The accuracy of the disease affects the diagnosis of the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation method of H-E staining solution of the present invention is:

[0021] For every 1000ml of improved hematoxylin staining solution, sodium iodate 0.04g; hematoxylin color essence 3g; aluminum potassium sulfate 28g; absolute ethanol 260ml; formaldehyde 0.1g; Stir to dissolve.

[0022] Further, every 1000ml of improved eosin staining solution includes the following components: 1.6g of alcohol-soluble eosin, 200ml of absolute ethanol, 28g of aluminum potassium sulfate, 0.05g of sodium iodate, 0.6g of water-soluble eosin, and 0.3 g, 10ml of glacial acetic acid, 0.1g of formaldehyde, and deionized water as the balance, let the above-mentioned components be evenly stirred and dissolved.

[0023] The H-E staining solution prepared by the above examples reduces the cost of use and saves the time for sample preparation during use. The H-E staining solution provided is used in conjunction with liquid-based cytology experiments to stain uniformly and t...

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PUM

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Abstract

The invention provides an H-E staining solution for liquid-based cytology experiments, and relates to the technical field of biological preparations. The H-E staining solution includes a modified hematoxylin staining solution and a modified eosin staining solution. The modified hematoxylin staining solution includes sodium iodate, hematoxylin, aluminium potassium sulfate, absolute alcohol, formaldehyde, glacial acetic acid and water. The modified eosin staining solution includes alcohol-soluble eosin, absolute alcohol, aluminium potassium sulfate, sodium iodate, water-soluble eosin, phloxine,glacial acetic acid, formaldehyde and deionized water. By utilizing the H-E staining solution, the cost of use is reduced, time for sample preparation is saved, and the H-E staining solution in matchwith the liquid-based cytology experiments enables uniform staining, clear tissue structures after staining, and bright cell color, and facilitates observation and judgment in liquid-based cytology section production.

Description

technical field [0001] The invention relates to the technical field of biological preparations; in particular, it relates to an H-E staining solution for liquid-based cytology experiments. Background technique [0002] At present, the incidence of serious diseases such as various cancers, tumors, and tissue lesions is on the rise. How to effectively diagnose these diseases has gradually become a hot spot in pathological research. [0003] Liquid-based cytology technology can assist clinicians to detect the above-mentioned diseases early and formulate correct treatment plans, so that patients can recover as soon as possible and reduce pain, so it has attracted the attention of researchers and clinicians. [0004] One of the important steps of liquid-based cytology technology is to stain liquid-based cells, and H-E staining solution is one of the most commonly used staining methods. The sample cells stained with H-E staining solution have good transparency, clear structure, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 不公告发明人
Owner 武汉宏强医疗器械有限公司
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