The invention provides an H-E dyeing liquid for a pathological tissue dyeing machine, and relates to the technical field of biological preparations. The H-E dyeing liquid comprises modified hematoxylin dyeing liquid and modified eosin dyeing liquid, wherein the modified hematoxylin dyeing liquid comprises following components: sodium iodate, hematoxylin, aluminum potassium sulfate, anhydrous ethanol, formaldehyde, glacial acetic acid, and water; and the modified eosin dyeing liquid comprises following components: alcohol-soluble eosin, anhydrous ethanol, aluminum potassium sulfate, sodium iodate, water-soluble eosin, erythrosine, glacial acetic acid, formaldehyde, and deionized water. The using cost is reduced, the sample preparation time is shortened, the provided H-E dyeing liquid can evenly dye pathological tissues under the assistance of a pathological tissue dyeing machine, the structure of dyed pathological tissues is clear, the color of cells is bright, and the observation on slides of pathological tissues becomes easy in clinic.