Process for detecting concentration of nonionic surfactant in waste water using spirogyra

A surfactant and non-ionic technology, which is applied in the field of detecting the concentration of non-ionic surfactants in waste water by Spirogyra, can solve the problems of unsuitable large sample detection, failure to meet requirements, low sensitivity, etc., and achieve easy observation and judgment, Increased sensitivity and clear symptoms

Inactive Publication Date: 2005-07-06
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that there is no perfect ideal method for the determination of nonionic surfactants at present. The current method is in the qualitative category for the determination of nonionic surfactants, or its sensitivity is low during quantitative analysis, which cannot reach requirements, and all require special testing instruments, which brings inconvenience to the popularization and application of testing methods. It cannot be completed in general laboratories, and it is not suitable for large sample testing. It is necessary to study new testing techniques.

Method used

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  • Process for detecting concentration of nonionic surfactant in waste water using spirogyra
  • Process for detecting concentration of nonionic surfactant in waste water using spirogyra
  • Process for detecting concentration of nonionic surfactant in waste water using spirogyra

Examples

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example 1

[0036] Determination of Biodegradation Efficiency of Nonionic Surfactant AE (Alkylphenol Polyoxyethylene Ether) with Water Sponge

[0037] In order to study the biodegradability of nonionic surfactants, the surfactant degrading strains were inoculated in the nonionic surfactant AE (alkylphenol polyoxyethylene ether) solution with an initial concentration of 2000mg / L, and the strains were inoculated in a 250mL cone. After 48 hours of shaking culture in a shaker flask at 37°C, the remaining AE concentration was determined.

[0038] 1. Preparation of materials

[0039] Nonionic surfactant standard sample: alkylphenol polyoxyethylene ether, analytically pure, purchased from Sigma Company (U.S.);

[0040] Spirogyra: It was taken from a ditch not polluted by toxic substances in the suburbs of Changsha City. Spirogyra is in good growth state and is in the vegetative growth period. After washing with distilled water, place it in a large beaker (1000 mL) filled with distilled water f...

example 2

[0057] Determination of the concentration of non-ionic surfactants in wastewater from a chemical plant for daily use

[0058] 1. Preparation of materials

[0059] Nonionic surfactant standard sample: alkylphenol polyoxyethylene ether (AE), analytically pure, purchased from Sigma Company (U.S.);

[0060] Spirogyra: It is taken from a ditch not polluted by toxic substances in the suburbs of Changsha. Spirogyra grows well and is in the vegetative growth period. After washing with distilled water, place it in a large beaker (1000 mL) filled with distilled water for later use.

[0061] 2. Determination of non-response critical concentration

[0062] ①. Preliminary determination of the non-response critical concentration of Spirogyra: Dilute the non-ionic surfactant standard sample AE in a 50mL small beaker with distilled water to prepare 0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, Concentration gradients of 7.0 and 8.0 mg / L; use tweezers to take about 5 g of sponge, absorb the external m...

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Abstract

This invention relates to a method to test the non-ion surface activity agent concentration in the waste water by use of water sponge, which comprises the following steps: using spirogyra sponge as target object; diluting the non-ion surface activity agent standard sample and adding the water sponge in growth and observing the water sponge cell structure changes under microscope to find out the threshold concentration; diluting the sample and adding small quantity of water sponge and observing the water sponge cell damage situations by microscopes; getting the non-ion surface activity agent concentration by the formula depicted.

Description

Technical field: [0001] The invention relates to a method for measuring the concentration of a nonionic surfactant, in particular to a method for detecting the concentration of a nonionic surfactant in waste water by using Spirogyra sp. Background technique: [0002] Nonionic surfactants, like other types of surfactants, are extremely important fine chemical products and are widely used in various civil and industrial sectors. Surfactant molecules have special amphiphilic groups and can change the physical properties of the two-phase interface, so they have a variety of composite functions, such as cleaning, foaming, wetting, emulsifying, solubilizing, dispersing, etc., therefore, enjoy the " The reputation of "industrial monosodium glutamate". According to statistics, the output of all surfactants in the world was 13 million tons in 1982, and by 1987, the output of surfactants in the world was more than 15 million tons. In the chemical industry in the United States, the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N1/28G01N33/00
Inventor 曾光明刘红玉鲁双庆袁兴中张惠英汤琳
Owner HUNAN UNIV
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