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Pasteur staining solution for cell preparation staining integrated machine

A technique of Papanicolaou staining and staining solution, applied in the field of Papanicolaou staining solution, which can solve the problems affecting the accuracy of pathological reading, complex preparation of Papanicolaou staining solution, and unstable staining effect, etc., to achieve bright cell coloring, easy promotion, Inexpensive effect

Inactive Publication Date: 2019-02-26
武汉宏强医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The problem to be solved by the present invention is that the preparation of the traditional Papanicolaou staining solution is relatively complicated, the staining solution is easy to be old, and the dyeing effect is unstable, and the effect is not good when combined with a high degree of automation. Accuracy, affects disease diagnosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation method of Papanicolaou staining solution of the present invention is:

[0021] For every 1000ml of improved hematoxylin staining solution, sodium iodate 0.04g; hematoxylin color essence 3g; aluminum potassium sulfate 28g; absolute ethanol 260ml; formaldehyde 0.1g; Stir to dissolve.

[0022] Further, every 1000ml of EA50 / OG composite staining solution includes the following components: bright green 0.6g; orange G 6g; eosin 3g; phosphotungstic acid 5g; methanol 170ml; glacial acetic acid 30ml; glycerol 110ml; purified water 80ml ; The balance is absolute ethanol, and the above-mentioned components can be evenly stirred and dissolved.

[0023] The Papanicolaou staining solution prepared by the above examples reduces the cost of use and saves the time for preparing samples during use. The Papanicolaou staining solution provided is combined with the all-in-one machine for film production and dyeing to dye evenly and the cell structure after sta...

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PUM

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Abstract

The invention provides a pasteur staining solution for a cell preparation staining integrated machine, which relate to the technical field of a biological preparation. The pasteur staining solution comprises an improved hematorylin staining solution and an EA50 / OG composite staining solution. The modified hematoxylin staining solution includes the following components: sodium iodate, hematoxylin,aluminium potassium sulfate, absolute ethanol, formaldehyde, glacial acetic acid, and water. The EA50 / OG composite staining solution comprises the following components: brilliant green, orange G, eosin, phosphotungstic acid, methanol, glacial acetic acid, glycerol, absolute ethanol and water. The pasteur staining solution reduces the use cost and saves the time for preparing a sample, and providesthe pasteur staining solution is combined with the cell preparation staining integrated machine to realize uniform staining, the cell structure after staining is clear, the cell staining is bright, and the solution is easy for clinical observation and judgment of liquid-based thin layer cell preparation.

Description

technical field [0001] The invention relates to the technical field of biological preparations; in particular, it relates to a Papanicolaou staining solution used in a film-making and dyeing integrated machine. Background technique [0002] At present, cervical cancer, tuberculosis, pleurisy, urinary tract infection and other diseases have become common clinical diseases and frequently-occurring diseases in my country. The incidence of these diseases is high and the cure time is long. The most important thing in life, how to effectively diagnose these diseases has gradually become a hot spot in pathology research. [0003] Liquid-based thin-layer cytology technology can assist clinicians to detect the above-mentioned diseases early and formulate correct treatment plans, so that patients can recover as soon as possible and reduce pain, so it has attracted the attention of researchers and clinicians. [0004] One of the important steps of liquid-based thin-layer cytology techn...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 不公告发明人
Owner 武汉宏强医疗器械有限公司
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