Method for identifying honeysuckle and lonicerae flos by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry

A technology of high performance liquid chromatography and liquid mass spectrometry, which is applied in the field of identification of varieties of Chinese herbal medicines, can solve the problems of insufficient speed of inspection, complicated inspection methods, and many influencing factors, and achieve high sensitivity, strong specificity, and improved accuracy Effect

Pending Publication Date: 2020-05-26
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

This method requires the preparations containing Japonica japonicus or the preparation containing Japonica japonicus raw materials as a control to construct an acute oral inflammation model; there are defects such as complex testing methods, not fast enough testing, high cost, many influencing factors, and large errors
[0006] Although there are reports in the prior art that use the ratio of the content of chlorogenic acid to strychnic acid or the single index of the content of Dipsacus saponin B as the identification basis, the premise of its application must be that the sample for character observation is a single herbal decoction piece. It can be applied, but the identification of mixed medicinal materials or medicinal materials whose properties cannot be observed is limited

Method used

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  • Method for identifying honeysuckle and lonicerae flos by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry
  • Method for identifying honeysuckle and lonicerae flos by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry
  • Method for identifying honeysuckle and lonicerae flos by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 establishes detection method

[0040] 1. Instruments and materials

[0041] 1.1 Instrument

[0042] TSQ-TUANTOM liquid mass spectrometry instrument (THERMO FISHER company), electrospray ionization source (ESI) mass spectrometer detector, waters C18 chromatographic column (2.1×10mm, 1.7μm);

[0043] Agilent 1260 Quaternary HPLC, Agilent G4212B Diode Array Detector, Agilent Eclipse XDB-C 18 Chromatographic column (250mm×4.6mm, 5μm);

[0044] KH500B ultrasonic cleaner (Kunshan GRAD), clear D24UV ultrapure water system (Millipore China).

[0045] 1.2 Materials and reagents

[0046] Acetonitrile (Fisher Scientific) was of chromatographic grade, water was of primary ultrapure water, absolute ethanol (COMIO) and other reagents were of analytical grade; formic acid and ammonium formate were of mass spectrometry grade.

[0047] Broken strychnic acid reference substance was purchased from Shanghai Shidande Standard Technology Service Co., Ltd., batch number: 600...

Embodiment 2

[0143] Embodiment 2 verification experiment

[0144] 1. The content of strychnic acid and chlorogenic acid in honeysuckle from different origins and batches

[0145] Each batch of honeysuckle from Hebei Province, Henan Province and Shandong Province was taken as the test sample and tested according to the detection conditions of the method described in Example 1, and the obtained detection results are shown in Table 13.

[0146] Table 13 Detection results of each batch of honeysuckle in Hebei Province, Henan Province and Shandong Province

[0147]

[0148]

[0149] 2. The content of strychnic acid and chlorogenic acid in different origins and batches of mountain silver flowers

[0150] Each batch of Sanyinhua in Guizhou Province, Hunan Province, Guangxi Province, Guangdong Province, and Sichuan Province was used as the test sample and tested according to the detection conditions of the method described in Example 1. The obtained detection results are shown in Table 14. ...

Embodiment 3

[0162] Example 3 mixed sample (honeysuckle and mountain silver flower mass ratio is 1:1 mixed) determination

[0163] Mixed sample preparation: according to Table 15, take equal weights of Flos Lonicerae and Flos Lonicerae respectively, pulverize, mix, as the test sample, refer to the determination of chlorogenic acid, cychnicic acid, and diapsus saponin B under the item of Example 1 The method was used to determine and calculate the content ratio of chlorogenic acid and stanchanic acid and the content of Dipsacus saponin B. The results are shown in Table 16.

[0164] Table 15 Composition table of mixed samples of honeysuckle and mountain silver flower (mass ratio is 1:1)

[0165] Mixed sample number Honeysuckle variety name Mountain silver flower variety name composition mass ratio 1 The first batch of first class in Henan 3 Guizhou gray felt wool-1 1：1 2 The first batch of first class in Henan 14 Hunan Longhui Shanyinhua 1：1 3 The firs...

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Abstract

The invention belongs to the technical field of identification of source varieties of traditional Chinese medicinal materials, and relates to a method for identifying honeysuckle and lonicerae flos byutilizing high performance liquid chromatography and liquid chromatography-mass spectrometry. With the adoption of the method, when the test sample is one or two of honeysuckle or lonicerae flos, theweight percentage of the sessile nux vomica acid and the chlorogenic acid in the dried test sample is detected through high performance liquid chromatography, and the ratio of the weight percentage of the chlorogenic acid to the weight percentage of the sessile nux vomica acid is calculated; the weight percentage of dipsacus asperoside B is detected in the dry test sample through liquid chromatography-mass spectrometry. Each honeysuckle is identified by utilizing the weight percentage ratio of chlorogenic acid to sessile strychnolic acid and the weight percentage of dipsacus asperoside B. Themethod is not limited by test sample properties, can be used for identifying single medicinal materials of honeysuckle and lonicerae flos or a mixture of the honeysuckle and the lonicerae flos, can also be used for identifying the single medicinal materials of the honeysuckle and the lonicerae flos or the mixture of the honeysuckle and the lonicerae flos in Chinese patent medicines, effectively improves the identification accuracy, and provides a true and false identification basis for medicine production, circulation and use.

Description

technical field [0001] The invention belongs to the technical field of identification of source varieties of traditional Chinese medicinal materials, and relates to a method for identifying honeysuckle and mountain silver, in particular to a method for identifying honeysuckle and mountain silver by using high-performance liquid chromatography and liquid chromatography-mass chromatography. Background technique [0002] Honeysuckle and mountain silver flower are listed as two kinds of medicinal materials since the 2005 edition of "Pharmacopoeia of the People's Republic of China". Because the two original plant species are similar and the medicinal materials are similar in appearance and shape, it is difficult to distinguish them. Therefore, the sources of medicinal materials of honeysuckle and mountain silver flower are unclear and the varieties are confused. The phenomenon of pretending to be honeysuckle, but the material basis of the two is quite different, which directly af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/74G01N30/86
CPCG01N30/02G01N30/36G01N30/34G01N30/06G01N30/74G01N30/7266G01N30/8679G01N2030/027
Inventor 张芳张永清郑冰清张龙霏石鹏亮于晓徐文流翁少全
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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