18 results about "Dipsacus asperoides" patented technology

The invention relates to the technical field of teasel breeding, and discloses a teasel germplasm resource multi-character collaborative screening breeding method, which comprises the following steps: multi-omics data acquisition: extracting teasel germplasm leaf DNA (deoxyribonucleic acid), and capturing exon regions of disease resistance and metabolism related genes by adopting a targeted sequencing technology to obtain an SNP (single nucleotide polymorphism) marker matrix; the method comprises the following steps: collecting root extracts, detecting medicinal components through ultra-high performance liquid chromatography-mass spectrometry, and constructing a metabolite abundance matrix; measuring phenotype data of plant height, root length, root weight, root rot morbidity and survival rate under drought stress; key site and metabolite correlation analysis: constructing a Bayesian network containing SNP markers, metabolite and phenotypes by using a graph database, and carrying out cross validation through a leave-one-out method. The dipsacus asper germplasm resource multi-character collaborative screening breeding method aims at solving the problem that a traditional breeding method lacks systematic integration analysis of genomes and metabolic pathways in dipsacus asper multi-character collaborative improvement.

This invention discloses a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, its preparation method, and its application, belonging to the field of traditional Chinese medicine composition technology. The raw materials of this traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, by mass percentage, include 8%-12% extract of honeysuckle branches and leaves, 8%-12% extract of dipsacus root, 8%-12% chrysanthemum, 4%-6% mulberry leaves, 4%-6% licorice root, 0.8%-1.2% peppermint, and 50.8%-67.2% anhydrous glucose. It has good anti-inflammatory effects and can effectively alleviate inflammatory responses caused by LPS. In particular, it has good therapeutic and ameliorative effects on the clinically prevalent porcine reproductive and respiratory syndrome (PRRS) and infectious bronchitis in chickens, effectively reducing morbidity and mortality, and improving production performance.

This invention discloses a method for determining the content of a traditional Chinese medicine compound containing honeysuckle, isatis root, and scutellaria. The method includes the following steps: taking a test solution of the traditional Chinese medicine compound, a first reference solution, and a second reference solution for detection. The chromatographic conditions are as follows: using a chromatographic column packed with octadecyl-bonded silica gel; mobile phase A selected from one or more of acetonitrile, methanol, and tetrahydrofuran; and mobile phase B being an acidic aqueous solution, an alkaline aqueous solution, and / or a buffer salt aqueous solution. The traditional Chinese medicine compound includes honeysuckle, isatis root, scutellaria, indigo leaf, forsythia, platycodon, patchouli, and licorice. The reference standards are baicalin, ammonium glycyrrhizate, honeysuckle saponin B, and dipsacus saponin B. The detection method of this invention is simple, accurate, efficient, stable, precise, reproducible, convenient, and easy to master. It can effectively save energy and reduce analytical costs, while this technology is not disclosed in existing technologies.

The invention belongs to the technical field of health-care wine, and particularly relates to waist-strengthening and kidney-tonifying wine which is prepared from morinda officinalis, cistanche deserticola, eucommia ulmoides, ginseng, cornu cervi pantotrichum slices, gecko, teasel, rhizoma drynariae, rock candy and 50-degree rice wine through related processing. The medicinal materials are ground and ultrasonically extracted, so that the dissolution of functional main components in the traditional Chinese medicine powder can be promoted, the soaking time of traditional Chinese medicines can be shortened, the content of effective components in the wine can be increased, and the wine not only can keep the flavor of the wine and has higher palatability, but also has the effects of tonifying the kidney, strengthening the waist and knees and improving the immunity. And the health-care tea is suitable for long-term drinking of middle-aged and elderly people with symptoms of waist and knee pain, frequent urination at night, physical weakness and low immunity.

The invention discloses a traditional Chinese medicine composition for treating bone diseases and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared by taking black monkshood, raw semen strychni, datura stramonium, dragon's blood and sappan wood as monarch drugs and taking ground beeltle, centipede, scorpio, Jin snake, zaocys dhumnade, black ant, teasel, rhizoma cibotii, native copper, rhizoma drynariae, radix achyranthis bidentatae, radix salviae miltiorrhizae, raw radix aconiti and the like as ministerial drugs; according to etiology and pathogenesis of bone diseases including cervical spondylosis, scapulohumeral periarthritis, rheumatic arthritis, hyperosteogeny and the like, medicines are reasonably combined, according to the etiology and pathogenesis, warm dredging and attacking evil are taken as main medicines, worm medicines are used for searching collaterals and eliminating evil are taken as essential medicines, the liver and kidney are tonified, the muscles and bones are strengthened, and the evil is prevented from hurting the body resistance; the traditional Chinese medicine can achieve the effects of warming channels and expelling cold, dispelling wind and eliminating dampness, activating meridians to stop pain, searching collaterals and eliminating evil, tonifying the liver and the kidney and the like, can effectively treat various orthopedic diseases, can treat symptoms by attacking evil and dredging collaterals, can treat root causes by tonifying the liver and the kidney, and achieves the purpose of treating both symptoms and root causes.

This invention proposes a traditional Chinese medicine compound for treating ankylosing spondylitis of the kidney-deficiency and damp-heat type accompanied by gastrointestinal dysfunction, along with its preparation method, belonging to the field of traditional Chinese medicine technology. The compound consists of Smilax glabra, Bletilla striata, Paris polyphylla, Geranium wilfordii, Dioscorea hypoglauca, Patrinia scabiosaefolia, Dipsacus asper, Drynaria fortunei, Pyrola rotundifolia, Clematis chinensis, Pheretima aspergillum, Lycopodium clavatum, Corydalis yanhusuo, Paeonia lactiflora, Atractylodes macrocephala (fried), Coix lacryma-jobi (raw), Aucklandia lappa, and Glycyrrhiza uralensis. This invention combines these herbs to achieve a synergistic effect, collectively clearing heat and dampness, strengthening the spleen and kidneys, and unblocking the meridians. The overall formula addresses both strengthening the body's foundation and eliminating pathogenic factors, ensuring unobstructed meridian flow and relieving arthralgia. This compound has the functions of clearing heat and dampness, strengthening the spleen and kidneys, and unblocking the meridians. It offers a short treatment course, rapid efficacy, low recurrence rate after cure, few side effects, and is inexpensive and convenient to use for treating ankylosing spondylitis with gastrointestinal dysfunction.

The invention provides Chinese herbal medicine oil for improving body immunity, and relates to the technical field of traditional Chinese medicine preparation. The Chinese herbal medicine oil for improving body immunity comprises mint, girald daphne bark, centella asiatica, berchemia sinica, saffron crocus, flos rhododendri mollis, semen strychni, radix aconiti agrestis, vernonia graveolens, ginger, caulis spatholobi, rhizoma drynariae, angelica sinensis, radix aconiti, frankincense, myrrh, gastrodia elata, ligusticum wallichii, radix angelicae pubescentis, cortex acanthopanacis, codonopsis pilosula, radix clematidis, asarum, elecampane, rhizoma atractylodis macrocephalae and teasel. By following the compatibility principle of monarch, minister, assistant and guide in traditional Chinese medicine, the formula contains multiple medicinal materials capable of strongly expelling wind and removing cold and activating meridians to stop pain, and also contains medicinal materials capable of promoting blood circulation to remove blood stasis and tonifying liver and kidney, purgation and tonification are combined, both symptoms and root causes are treated, the pathogenesis of arthralgia is very accurate, the immunity of a human body can be improved, and by using the whole links of soaking, frying, filtering, squeezing and aging, the arthralgia treatment effect is improved. About 15%-20% of medicinal oil adsorbed in the medicinal materials can be recycled, the overall quality is improved, the properties of the medicinal materials can be fused through curing, the medicinal materials become mellow, and irritation is reduced.

The invention provides application of a dipsacus asper and radix cyathulae stem and leaf compound in a feed additive, and belongs to the technical field of feed. Dipsacus asper stem and leaf powder, radix cyathulae stem and leaf powder and corn flour are compounded, and the feed additive is prepared through double fermentation of bacillus subtilis and lactobacillus plantarum. The fatty acid synthase expression level of fattening pigs fed with the feed additive is remarkably reduced. The lean meat percentage of a control group is 62.75%, the lean meat percentage of a group fed with a conventional traditional Chinese medicine feed additive is 64.35%, the lean meat percentage of a group fed with a fermented traditional Chinese medicine feed additive is 67.87%, and the lean meat percentage is remarkably improved. The pig fattening period of the control group is 104 days, and the feed conversion ratio is 3.02: 1; the fattening period of the pigs fed with the conventional traditional Chinese medicine feed additive group is 101 days, and the feed conversion ratio is 2.94: 1; the fattening period of pigs fed with the fermented traditional Chinese medicine feed additive is shortened to 88 days, the feed conversion ratio is 2.57: 1, and the breeding efficiency is remarkably improved.

The invention discloses teasel glycosyl transferase and application thereof in production of alpha-hederaoside and teasel saponin B. The amino acid sequence of the teasel glycosyl transferase is shown as SEQ ID NO.1, and the nucleotide sequence of the coding gene of the teasel glycosyl transferase is shown as SEQ ID NO.2. The invention further discloses a preparation method of the teasel glycosyl transferase. After the glycosyl transferase is subjected to prokaryotic expression, a series of triterpenoid saponins can be catalyzed to be converted into triterpenoid saponin-3-(2-O)-glycoside products in a glycosylation manner, and the triterpenoid saponin-3-(2-O)-glycoside products comprise alpha-hederin and dipsacus asper saponin B. The discovery of the dipsacus asper triterpenoid saponin glycosyltransferase provides more optional biological elements for the biosynthesis of natural products, and has a good application prospect.

The invention discloses a novel application of a Guangkang capsule medicine. According to the application of the Gukang capsule medicine in preparation of the medicine for treating gout, the medicine for treating gout is a medicine preparation prepared from an extract obtained by mixing and decocting 570 g of roots of Japanese banana, 400 g of creeping oxalis, 600 g of fructus psoraleae and 300 g of radix dipsaci with water, 150 g of pseudo-ginseng fine powder, 30 g of fine powder of the roots of Japanese banana and pharmaceutically acceptable auxiliary materials. Animal experiments show that the serum uric acid level of a hyperuricemia model mouse can be remarkably reduced, and joint swelling and inflammatory cell infiltration are relieved. Clinical observation further proves that the traditional Chinese medicine composition has a good curative effect on improvement of arthralgia, limited activity and other symptoms of gout patients, is relatively high in safety and does not have obvious adverse reaction.

The application relates to the field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition, a traditional Chinese medicine preparation, a preparation method and application thereof. The traditional Chinese medicine composition contains angelica sinensis, safflower, red peony root, salvia miltiorrhiza, radix aconiti kusnezoffii, radix aconiti carmichaeli, cassia twig, drynaria, radix rubi sylvestris, frankincense, myrrh, radix rubi sylvestris, natural copper, elderberry, radix curdlane, crab shell and zhangdan. The preparation method of the traditional Chinese medicine preparation comprises the following steps: mixing angelica sinensis, safflower, red peony root, salvia miltiorrhiza, radix aconiti kusnezoffii, radix aconiti carmichaeli, cassia twig, drynaria, radix rubi sylvestris, radix rubi sylvestris, natural copper, elderberry, radix curdlane and crab shell with vegetable oil to perform frying and boiling, performing solid-liquid separation to obtain medicine oil; mixing the medicine oil after being refined with zhangdan, frankincense and myrrh to obtain an ointment; soaking the ointment in water, taking out, heating and melting, mixing with blood ointment powder, and spreading. The traditional Chinese medicine composition and the traditional Chinese medicine preparation have the effects of setting bone, continuing tendon and relieving swelling and pain.

The invention discloses a method for simultaneously detecting contents of various active ingredients in Sanbi decoction based on HPLC (High Performance Liquid Chromatography), and belongs to the technical field of analytical chemistry. The method for determining the content of loganic acid, gentiopicroside, 5-O-methylvisammioside, dipsacus asper saponin VI and glycyrrhizic acid in the Sanbi decoction is successfully constructed by virtue of an HPLC (High Performance Liquid Chromatography) gradient elution technology. A content determination result shows that in 15 batches of samples, the content ranges of the five active ingredients are respectively 1.6584 mg / g to 4.0230 mg / g, 1.1120 mg / g to 4.0518 mg / g, 0.1688 mg / g to 0.4344 mg / g, 2.7441 mg / g to 10.3693 mg / g and 2.6289 mg / g to 5.0324 mg / g. According to the Chinese herbal medicine compound preparation pharmaceutical research technical guidance principle (trial) managed according to the ancient classical famous prescription catalog, the content ranges all meet the standard requirement of the mean value + / -3SD. Moreover, the method provided by the invention has good accuracy and reliability, and a scientific basis can be provided for the quality control and evaluation of the substance standard of the Sanbi decoction.

The invention belongs to the technical field of biological medicines, and particularly relates to application of dipsacus asper saponin B in preparation of a medicine for treating or preventing inflammatory bowel diseases. The technical problem to be solved by the invention is to provide a new choice for treatment of colitis. The technical scheme of the invention is application of dipsacus asper saponin B in preparation of drugs for treating or preventing inflammatory bowel diseases. The invention defines that the dipsacus asper saponin B has a treatment effect on colitis, systematically explains the mechanism of the dipsacus asper saponin B, and provides a brand new scientific basis and data support for development of the dipsacus asper saponin B as a medicine for treating inflammatory enteritis.

The invention discloses teasel glycosyl transferase and application thereof in production of clematis saponin A. The amino acid sequence of the teasel glycosyl transferase is shown as SEQ ID NO.1, and the nucleotide sequence of a coding gene of the teasel glycosyl transferase is shown as SEQ ID NO.2. The invention further discloses a preparation method of the teasel glycosyl transferase. After the glycosyl transferase is subjected to prokaryotic expression, a series of triterpenoid saponins can be catalyzed to be converted into triterpenoid saponin-3-O-glycoside products through glycosylation, and the triterpenoid saponin-3-O-glycoside products comprise radix clematidis saponin A. The discovery of the dipsacus asper triterpenoid saponin glycosyltransferase provides more optional biological elements for the biosynthesis of natural products, and has a good application prospect.

The invention provides a traditional Chinese medicine composition and application thereof in preparation of medicines for treating or preventing hyperuricemia, gout, uric acid kidney stone, gouty arthritis and gouty nephropathy. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5 to 25 parts of raw radix astragali seu hedysari, 5 to 15 parts of radix morindae officinalis, 5 to 15 parts of radix dipsaci, 5 to 15 parts of flos lonicerae, 5 to 25 parts of herba hedyotis diffusae, 5 to 25 parts of terra flava usta, 5 to 25 parts of poria peel, 5 to 15 parts of radix puerariae, 5 to 15 parts of fructus rubi, 5 to 15 parts of fructus schisandrae chinensis, 5 to 15 parts of fructus akebiae and 5 to 15 parts of raw radix glycyrrhizae. Experiments show that after the traditional Chinese medicine composition is used for treatment, the symptoms of a patient are relieved, the uric acid value is reduced, liver and kidney function damage is avoided, and the effect is better than that of existing western medicines.

The application discloses a traditional Chinese medicine composition for senile dementia and stroke and a preparation method thereof, which is prepared from the following raw medicinal materials in parts by weight: 40 parts of Schinus molle, 10 parts of American ginseng, 8 parts of Panax notoginseng, 5 parts of hawthorn, 5 parts of Chinese yam, 5 parts of Herba Epimedii, 6 parts of Radix Curcumae, 5 parts of Poria cocos, 5 parts of Astragalus membranaceus, 5 parts of Codonopsis pilosula, 3 parts of Platycladus orientalis, 3 parts of Corydalis, and 5 parts of Cannabis sativa Linn. The application has the advantages that a nerve blood vessel unit system regulation mechanism is creatively proposed, the limitation of single target treatment is broken through, the Schinus molle, which is the monarch drug in the prescription, is rich in nervonic acid and neurotrophic factor-like substances, can directly act on neuron mitochondrial energy metabolism, improves brain tissue energy supply, and on the process level, the multi-stage dynamic extraction technology of the application innovates the traditional decocting mode.

The invention provides a traditional Chinese medicine compound for treating kidney-deficiency damp-heat type ankylosing spondylitis with gastrointestinal dysfunction and clearing away heat, eliminating dampness and dredging governor meridian and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine compound is prepared from rhizoma smilacis glabrae, rhizoma bletillae, rhizoma paridis, geranium wilfordii, rhizoma dioscoreae hypoglaucae, herba patriniae, teasel, rhizoma drynariae, herba pyrolae, radix clematidis, lumbricus, herba lycopodii, rhizoma corydalis, radix paeoniae rubra, roasted rhizoma atractylodis macrocephalae, raw semen coicis, costus root and liquorice. The medicines are combined, so that the effects of the medicines generate a synergistic effect, and the effects of clearing heat, eliminating dampness, tonifying spleen, tonifying kidney and dredging governor meridian are achieved together. The whole compatibility has the effects of consolidating root and eliminating evil, so that meridians and collaterals are dredged, and the arthralgia syndrome is solved. The traditional Chinese medicine compound has the functions of clearing heat, eliminating dampness, invigorating spleen, tonifying kidney and dredging governor meridian, and has the advantages of short treatment course, quick effect, low possibility of relapse after curing, small side effect, low price and convenience in use when being used for treating the ankylosing spondylitis accompanied by gastrointestinal dysfunction.

The invention provides a traditional Chinese medicine composition for treating endometrial injury as well as a pharmaceutical preparation and application thereof, dandelion, sargentgloryvine stem, Chinese violet, patrinia herb for eliminating dampness and heat, artemisia anomala, radix bupleuri, corydalis tuber, szechwan chinaberry fruit for dredging Chong and Ren meridians, teasel and mistletoe for clearing and nourishing liver and kidney are selected, so that the dampness and heat are cleared, the Chong and Ren meridians are smooth in qi activity, the evil is removed, and the disease treatment is realized. A treatment result shows that the formula disclosed by the invention can improve the endometrial state by improving the blood flow of the uterine artery; tracking of pregnancy preparation information of a patient finds that the natural pregnancy rate of the patient treated by the traditional Chinese medicine composition within half a year is significantly higher than that of a control group (a female progestational hormone continuous therapy treatment group), the treatment thought of clearing and nourishing liver and kidney and dredging Chong and Ren channels can improve the intimal thickness of the patient, and it is possible to improve the intimal thickness of the patient by improving uterine artery blood perfusion. Finally, the endometrial receptivity is improved, and the pregnancy rate is increased. Therefore, the invention provides evidence-based basis and clinical guidance for the treatment of the recurrent abortion endometrial injury clinically.