102results about How to "Strong broad spectrum" patented technology

The invention discloses a method for growing watermelons. The method includes the steps of processing seeds and accelerating germination, sowing and raising seedlings, applying base fertilizer on the whole land, transplanting the seedlings, performing field management, harvesting and the like. The method for growing watermelons is easy to operate, convenient to manage, good in growth vigor of watermelons, high in disease resistance, uniform in result nature and high in yield, the yield per mu can reach 4000 kg to 5000 kg, the watermelons are large, good in quality, high in sugar content, bright in flesh color, delicate in flesh and high in water content and commodity value, and therefore the method has great significance for increasing income of melon farmers.

The invention belongs to the field of molecular biological gene engineering, and particularly relates to a double-control double-regulation prokaryotic expression vector system and a construction method and application thereof. Two expression vectors finish expression of a target gene in the vector system, wherein one expression vector is a main expression vector, and the target gene is cloned on the expression vector; the other expression vector is an auxiliary expression vector, and the auxiliary expression vector controls a switch for regulating the promoter of the main expression vector; the main expression vector contains an SP6 promoter and a lactose regulating gene; the auxiliary expression vector contains an arab promoter, an araC regulating gene and an SP6RNA polymerase gene; and the target gene expressed by the main expression vector and the auxiliary expression vector are controlled and/or regulated by an inducer.

The invention belongs to the field of bioengineering, and provides a bacteriophage with environment disinfection capability and applications thereof. The bacteriophage monomer is escherichia coli bacteriophage, has a latin name of E.coli phage, and is named Bp7, and has broad-spectrum bactericidal capability to escherichia coli, the bacteriaophage Bp7 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on 6th, June, 2012 and has a preservation number of CGMCC No.6201; the bacteriophage has strong cracking effect on escherichia coli, provides a bacteriophage source for industrial production of bacteriophage and can be used for disinfection; and the bacteriophage can also be used as a safe biological disinfectant for environment disinfection, thus controlling aquaculture ppollution and laboratory pollution; the bacteriophage can be used for industrialization production, can be amplified by specificity of host bacteria Escherichia coli, and can be used as a biological disinfectant for laboratory environment disinfection and chicken house environment disinfection.

The invention discloses a difluoromethylene-containing compound and a preparation method thereof. The preparation method of the difluoromethylene-containing compound is characterized in that a Suzuki coupling reaction is carried out on a compound A and a compound B in a solvent in the presence of an alkali, a ligand and a catalyst to obtain a compound C, the catalyst is a nickel salt, and the nickel salt is NiQ2.mH2O, NiLnCl2, NiLnBr2, NiLnI2 or NiLn(OH)2. The preparation method has the advantages of simple and easily available raw materials, few reaction steps, high conversion rate, simple post-treatment operation, low price and low dosage of the catalyst, good compatibility, strong broad spectrum activity, avoiding of use of severely toxic reagents, low production cost and good market application prospect.

The embodiment of the invention discloses a method for preparing a two-dimensional material. The two-dimensional material is prepared by stripping through a cutting force generated by secondary flow. The method comprises the following steps of selecting a raw material and a dispersing liquid, and preparing a mixed solution in a mixer; selecting a reactor and a power system, and connecting the reactor, the power system and the mixer; and pushing the mixed solution to the reactor, controlling the flow rate and the circulation time in the reactor, and stripping the raw material to prepare the two-dimensional material by rapid flowing of the mixed solution in the reactor and the cutting force generated by the continuous secondary flow formed at a bent plate. The preparation method has the advantages of simplicity in operation process, low cost, high adjustability and high universality and practicability, and is suitably used for various dispersing liquids and raw material systems; and infinite amplification in longitudinal dimension can be achieved during the preparation process, thus, an amplification effect does not exist, industrial production is promoted, and the method can be used for preparation of the two-dimensional material at a large scale.

The invention provides a method for treating coking biochemical effluent by microwave-oxidation-coagulation technology, which comprises the following steps of: A, adding a mixed reagent consisting of H2O2 and Fe2+ into the coking biochemical effluent until the mass concentration of hydrogen peroxide in water is between 0.01 and 1 percent, and controlling the pH to between 2.0 to 6.0; B, delivering the mixed liquid into an industrial microwave oven for processing for 5 to 60s; C, regulating the pH value of the mixed liquid to between 6.0 and 6.5 or 7.5 and 8.5, and carrying out oxidation treatment for 1 to 4 hours under an aeration condition that the aeration rate is 10 to 40m<3>/h; D, adding a coagulant into the liquid until the mass concentration of the coagulant in the liquid is betweenone millionth and one hundred thousandth; E, precipitating and separating; F, filtrating the liquid to obtain process effluent for recycling; and G, collecting sludge, separating the liquid from the sludge; and returning the liquid to the step D, and filter-pressing the sludge to obtain sludge cakes. The method realizes recycling, does not discharge sewage, and effectively solves the problem thatpresent coking sewage is difficult to treat and to recycle.

The present invention discloses a primer set for detecting mycoplasma. The primer set comprises a forward primer having a sequence represented by SEQ ID No.1; and a reverse primer having a sequence represented by SEQ ID NO.2. The invention further discloses a kit for detecting the mycoplasma by using the primer set, uses of the kit in detection of mycoplasma pollution, and a detection method for detecting the mycoplasma pollution by using the kit. The kit of the present invention has advantages of simple operation, broad spectrum, strong specificity and high sensitivity, can rapidly detect the mycoplasma pollution, and has wide application prospect.

The invention relates to an artificial antigen and antibody that is used for analyzing sulfa plural residual immunity and the manufacturing method. It uses 4-(4- amino benzene sulfonyl) benzoic acid as hapten and compound artificial antigen with carrier protein and taking animal immunity to make antibody that could be used to rapidly test the residual to sulfa. It has good broad spectrum feather and has great application prospect.

The invention discloses a monofluoroalkyl-containing compound and its preparation method. The preparation method comprises that in a solvent, in the presence of an alkali, an additive, a ligand and a catalyst, a compound A and a compound B undergo a suzuki coupling reaction to produce a compound C. The catalyst is a nickel salt with a chemical formula of NiLnCl2, NiLnBr2, NiLnI2 or NiQ2. mH2O. The preparation method introduces monofluoroalkyl through a one-step reaction, is efficient and simple, has good function group compatibility and good broad spectrum activity, is free of a highly toxic reagent, has simple and safe aftertreatment processes, is environmentally friendly, utilizes a small amount of a cheap catalyst, allows mild reaction conditions, realizes a high reaction conversion rate, a high yield and a low production cost and is suitable for industrial production.

The invention discloses an antibacterial flame-retardant needle-punched compound nonwoven fabric preparation method. An antibacterial flame-retardant needle-punched compound nonwoven fabric has advantages of high structural strength and texture compactness, environmental friendliness, safety, reliability, convenience in manufacturing, wide application range and the like and provides a cottony comfortable handfeel. Antibacterial microcapsules are uniformly distributed in an antibacterial layer, excellent broad-spectrum, slow-release and bactericidal effects are achieved, drug tolerance and toxicity are avoided, and antibacterial and bacteriostatic effects are effectively achieved due to adding of nano zinc oxide and chitosan. Owing to adding of essence aromatic substances, the microcapsulescontinuously emit aroma in a utilization process to mask odors, and a high popularization and application value is achieved. The practice proves that the antibacterial effect is still kept even the fabric is washed for multiple times, and the antibacterial nonwoven material is promising in prospect.

A demulsifying dehydration method of an oilfield produced fluid comprises a step that the demulsifying dehydration is carried out through mixing the oilfield produced fluid, a demulsifying assistant and a demulsifying agent. The oilfield produced fluid contains an emulsified viscosity reducer; the application amount of the demulsifying assistant is 10-200ppm on the basis of the quality of the oilfield produced fluid; and the demulsifying assistant has a structure represented by a general formula of [R1P<+>(R2)3]nX<n->, R1 is a C6-22 alkyl or alkoxy group, R2 is a C1-4 alkyl, hydroxyalkyl, phenyl, benzyl or tolyl group, n is an integer between 1 and 3, X is Cl<->, Br<-> or HCOO<-> when n is 1, X is SO4<2-> when n is 2, and X is PO4<3-> when n is 3.

A purpose of the present invention is to provide a deep oxidation treatment apparatus for difficultly-degraded organic chemical industry wastewater so as to solve bottleneck problems of low treatment efficiency, poor stability, excessive CODcr in effluent, high equipment investment, large occupation area, high operation cost and the like of the conventional deep treatment method after the organic chemical industry wastewater is subjected to the conventional two-stage biochemistry treatment. The present invention further provides a deep oxidation treatment method for difficultly-degraded organic chemical industry wastewater through combination of acidification bacterial removal, two-stage enhanced electrochemistry (micro-electrolysis) and two-stage heterogeneous catalysis (Fenton-like) oxidation. The apparatus comprises an acidification bacterial removal tank, a first-stage enhanced electrochemistry reactor, a second-stage enhanced electrochemistry reactor, a first-stage heterogeneous catalysis oxidation reactor and a second-stage heterogeneous catalysis oxidation reactor, wherein the acidification bacterial removal tank, the first-stage enhanced electrochemistry reactor, the second-stage enhanced electrochemistry reactor, the first-stage heterogeneous catalysis oxidation reactor and the second-stage heterogeneous catalysis oxidation reactor are sequentially connected through pipelines, the connection pipeline between the acidification bacterial removal tank and the first-stage enhanced electrochemistry reactor is provided with a lift pump, and the second-stage heterogeneous catalysis oxidation reactor is connected with a water outlet pipe.

The invention provides a glucosamine compound shown as the general formula I and application of pharmaceutically acceptable salt or solvate in preparation of the antiviral drug. The compound has excellent antiviral effect, particularly the broad spectrum property of the antiviral effect is remarkable, and the glucosamine can be used for preparing the antiviral drug. (See the general formula in thedescription.).

The invention discloses a Chinese herb extract with an antibacterial effect. The Chinese herb extract is prepared by decocting the following raw materials in parts by weight with water and performing alcohol extraction: 120-180 parts of ciderage, 120-180 parts of pogostemon cablin, 120-180 parts of glossy privet fruit and 80-120 parts of astragalus membranaceus. An in-vitro antibacterial test result shows that the Chinese herb extract disclosed by the invention is good in antibacterial effect and has broad spectrum activity; compared with a water extract, a microwave extract and an ethanol fractionated extract, the Chinese herb extract is better in antibacterial effect. The Chinese herb extract is used as an antibacterial medicament for poultry substitute antibiotics and chemical medicaments, so that pollution, caused by drug resistance and drug retaining, to a poultry product can be effectively avoided; the poultry harmful excrements, the protein use amount and the nitrogen pollution are reduced; the Chinese herb extract has a wide market prospect.

The invention relates to a dehydration method of oilfield produced fluid. According to the invention, oilfield produced fluid, quaternary ammonium salt and a demulsifying agent are mixed, such that demulsification dehydration is carried out. The oilfield produced fluid contains an emulsification viscosity reducer. According to the mass of the oilfield produced fluid as a reference, the dosage of quaternary ammonium salt is 10-300ppm. The cation portion of the quaternary ammonium salt has a structure represented by the formula below. R1 and R2 can be the same or different, and R1 and R2 are C8-C22 alkyl or C8-C22 substituted alkyl; R4, R5, R6, and R7 can be the same or different, and R4, R5, R6, and R7 are C1-C4 alkyl, C1-C4 alkoxy, polyoxyethylene with polymerization degree of 1-20, or polyoxyethylene/polyoxypropylene with polymerization degree of 1-20; and R3 is C2-C8 alkylene or diethylene maleate.

The invention discloses a fluorine methyl -containing compound and a preparation method thereof. The fluorine methyl-containing compound is as shown in a formula C, R is a group containing an aryl group, or a group containing a heteroaryl group, the aromatic carbon atoms on the aryl group or the heteroaryl group are connected with CFmHn, m is 1 or 2, and m + n is equal to 3. The compound is difficult to prepare according to the prior art, raw materials and a catalyst of the preparation method are common industrial raw materials, and are cheap and easy to obtain, the preparation method is highin reaction efficiency, high in yield, simple in post-treatment, low in toxicity, environment-friendly, good in functional group compatibility, wide in broad-spectrum property, low in production costand good in market application prospect.

The invention relates to the technical field of photocatalyst preparation and pesticide degradation, in particular to a method for synthesizing a regular-morphology unitary, binary or ternary sulfide-TiO2 composite nanostructure in a one-pot mode through a solvothermal method and application thereof in photocatalytic degradation of organic pesticides. Because the raw materials not only can providea titanium source, but also can provide a sulfur source, the sulphide-TiO2 composite nanostructure with regular morphology is prepared by adopting a solvothermal method one-pot synthesis technology.The sulphide-TiO2 composite nano-structure prepared by the preparation method disclosed by the invention has the advantages that active species are generated through optical excitation; toxic pesticides such as .OH and .O2- and the like can be decomposed into small molecules or non-toxic compounds, residues of the toxic pesticides are effectively reduced, the production safety is improved, variousorganic pesticides can be degraded, the broad spectrum property is high, the effect is good, and good application prospects are achieved in the aspects of pesticide greening, low residues and the like.

The invention relates to the field of biomedicine, relates to an envelope protein trimer immunogen capable of inducing HIV-1 broad spectrum and neutralizing antibody and application thereof, in particular to an envelope protein Env common gene sequence designed based on HIV-1 Chinese epidemic strain and a stable envelope protein gp120 trimer immunogen obtained based on the sequence. The inventionfurther relates to the application of the HIV-1 envelope protein gp120 trimer immunogen in preparation of AIDS vaccines.

The invention discloses a multifunctional biomass composite building material and a preparation method, wherein the building material is prepared from the following raw materials by weight: 100 partsof magnesium oxide; 30-40 parts of magnesium sulfate heptahydrate; 40-60 parts of a modifier; and 30-50 parts of a filling material; specifically, the weight of active magnesium oxide in the magnesiumoxide accounts for 50%-75% of the total weight of the magnesium oxide, the modifier is formed by mixing phosphoric acid, soda brick, an organic silicon compound, methyl/butyl acrylate and mica powderaccording to a weight ratio of 0.05-1:1-5:1-5:1-5:2-12, and by means of the mode, at least one defect in the background technology is overcome, and energy conservation, environmental protection and good economic benefits are achieved.

The invention discloses a biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables, belongs to the technical field of biocontrol bacterium, and particularly provides Streptomyces cacaoi FN. The preservation number of the biocontrol bacterium is CGMCC NO. 13080. The biocontrol bacterium has the functions of preventing and treating apple tree fungal diseases such as apple anthrax leaf blight and apple alternaria leaf spot and fungal diseases of other fruit trees such as tomato early blight and watermelon fusarium wilt, and can not only completely replace chemical prevention and treatment methods and eliminate potential safety hazard of food, but also has outstanding effects of effectiveness and antibacterial broad spectrum properties of apple tree fungal disease prevention and treatment. Meanwhile, the streptomycete has excellent properties of hereditary stability, broad spectrum bacterium resistance, thermal resistance, acid-base resistance, ultraviolet radiation resistance and the like, and can be adopted as an optimal biocontrol bacterium for preventing and treating fungal diseases of fruits and vegetables.

The invention relates to the technical field of food, in particular to a nutritional food according with a theory of yin-yang balance of five elements and a preparation method of the nutritional food. A key technology for matching natural food raw materials according with yin-yang balance of five elements is adopted, active ingredients comprising, in parts by weight, 15-22 parts of walnut kernels, 5-7 parts of wolfberry fruit, 4-6 parts of red dates without kernels, 10-15 parts of black sesame, 5-7 parts of lotus seeds with cores, 1.5-2 parts of hericium erinaceus, 3-4.5 parts of almonds, 7-10 parts of black beans, 6-9 parts of raisin and 0.5-1 parts of dried mulberry leaves are mixed to form a raw material, the raw material is put in a grinder, 160-260 parts by weight of water is added, the mixture is ground for 5 minutes, a paste is obtained, boiled for one minute and packed in a pop-top can, and the nutritional food according with the theory of yin-yang balance of five elements is obtained. The method is used for preparing the nutritional food. The nutritional food has functions of tonifying the spleen and the stomach, moistening the lung, strengthening the heart, protecting the liver and invigorating the kidney, is wide natural nutrition and health care convenient food suitable for men and women of all ages and can be widely and conveniently applied.