4results about How to "Strong broad spectrum" patented technology

The invention discloses a bacillus PY4 strain capable of efficiently antagonizing various phytopathogens and application of the bacillus PY4 strain, and belongs to the technical field of biological prevention and control of plant diseases. The strain has stable morphological characteristics, and is identified according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the strain is a Paenibacillus bacterium. An in-vitro confrontation test proves that the PY4 can effectively inhibit pathogenic fungi such as sclerotinia sclerotiorum, fusarium graminearum, pseudocauda sp, alternaria alternata and sclerotium rolfsii. Field application results show that the PY4 has a good prevention and treatment effect on sclerotinia rot of colza. The invention further provides a biocontrol agent based on the strain and an application method of the biocontrol agent. The biocontrol agent can be used for green prevention and control of soil-borne and leaf-borne fungal diseases of crops.

The application provides application of a nitrogen-doped carbon-based iron monatomic catalyst in degradation of ADC drugs in wastewater, and belongs to the technical field of advanced oxidation water treatment. The nitrogen-doped carbon-based iron monatomic catalyst with a two-dimensional wrinkled ultrathin morphology is prepared by a nano-magnesium oxide template assisted method. In application, the catalyst is dispersed in wastewater containing ADC drugs, stirred and adsorbed to adsorption equilibrium, then a proportion of persulfate is added, and reaction is carried out under the condition that pH is 3-9, so that efficient degradation of the ADC drugs is realized. The application has the advantages of high degradation efficiency, fast reaction speed, wide pH adaptation range, strong anion interference resistance, good broad-spectrum property, good catalyst stability and the like, and actual wastewater toxicity evaluation proves that the application has good detoxification capacity, and has wide application prospect in the field of treatment of refractory organic wastewater such as pharmaceutical wastewater and hospital wastewater.

This invention discloses the application of PACAP peptides as plant immunomodulators, belonging to the field of plant immunology technology. This invention is the first to apply PACAP to a plant system, discovering that it can act as a novel plant immunomodulator to regulate plant immune responses, thereby enhancing plant resistance to pathogenic microorganisms. Experimental results show that PACAP can rapidly induce intracellular calcium ion influx in Arabidopsis thaliana cells, activate the MAPK signaling pathway, and upregulate the expression of early immune marker genes such as FRK1. Furthermore, PACAP treatment significantly improves plant resistance to pathogens such as *Pseudomonas syringae* and *Ralstonia solanacearum*. This invention also confirms that not only does the full-length PACAP (1-38) possess plant immune-promoting activity, but its N-terminal truncated peptide (1-27) and receptor antagonist fragment (6-38) also have plant immune-inducing functions. This invention provides a new technical solution for the development of plant immune inducers.

The invention discloses a rabbit-derived single B cell antibody of porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein, a fusion protein, a detection kit and a detection method. The invention provides two rabbit-derived single B cell antibodies T-11 and T-12, wherein amino acid sequences of variable regions of a heavy chain and a light chain of the rabbit-derived single B cell antibodies T-11 and T-12 are respectively shown as SEQ ID No.1-4; also provided are PRRSV-1 type and type 2 nucleocapsid protein fusion proteins BiN-2 (SEQ ID No.5) and antibody compositions comprising T-11 and T-12. Based on the antibody and the fusion protein, a competitive ELISA detection method and a kit are established, T-11 is used for capturing BiN-2 to construct an indirect coating antigen, biotin-labeled T-12 is used as a competitive antibody, and a judgment result is detected through an enzymatic reaction and a light absorption value. The method can be used for simultaneously detecting the PRRSV-1 type and the PRRSV-2 type antibodies, the sensitivity and the specificity both reach 97% or above, the method has no cross reaction with other porcine virus antibodies, the operation is simple and convenient, the stability is good, and a reliable tool is provided for accurate prevention and control of PRRS and epidemiological monitoring.