The invention provides combined trace element and microorganism assembled water-soluble fertilizer, comprising a first fertilizer prepared from trace elements and microorganisms, and a second fertilizer prepared from macro elements, medium elements and trace elements; the first fertilizer comprises, in certain weight parts, Maifan stone, realgar, large bluestone, potassium feldspar, zeolite, trace elements including Cu, Fe, Mg, Mn, Zn, B, Mo and Co, Bacillus mucilaginosus, Bacillus subtilis, Bacillus licheniformis, Bacillus laterosporus, phosphorus-dissolving potassium-dissolving bacteria, polypeptide amino acids, nano carbon, and mineral-derived potassium fulvate; the second fertilizer is 20-20-20+Te water-soluble fertilizer or 30-10-10+TE water-soluble fertilizer or 15-15-30+TE water-soluble fertilizer. The combined trace element and microorganism assembled water-soluble fertilizer is convenient to use and low in cost, application for soil can be decreased, soil environment for the growth of crops is improved, soil-borne disease damage is prevented, the ability of crops to resist diseases and pests is enhanced, soil can immobilize effective ingredients less fixedly, fertilizer efficiency is improved and effect period is extended, immunity of crops is improved, and crop yield and quality are increased.