The invention provides a special fertilizer for single cropping rice. The special fertilizer is prepared by the following raw materials by weight: 10-20 parts of cottonseed meal, 8-10 parts of coated nitrogen-phosphorus-potassium mixed fertilizer, 25-40 parts of calcium superphosphate, 8-10 parts of coated potassium chloride, 20-30 parts of plant ash, 1-2 parts of ferrous sulfate, 1-2 parts of zinc sulfate, 0.1-0.3 parts of borax, 10-12 parts of medical stone, 5-7 parts of peat, 3-4 parts of celastrus orbiculatus powder, 3-4 parts of oleander powder, 10-15 parts of bagasse, 10-15 parts of dolomite, 18-22 parts of chicken manure, 3-4 parts of carboxymethylcellulose, 15-20 parts of 30% of sulfuric acid, 5-10 parts of lime, 10-15 parts of chicken feather and 4-6 parts of propanediol sebacate polyester. A preparation method of the single cropping rice comprises the steps as follows: carrying out acidolysis on hairs to obtain amino acid; generating an amino acid chelated micro or medium fertilizer solution; sintering the dolomite to prepare a fertilizer carrier; fermenting an organic fertilizer; mixing the nitrogen-phosphorus-potassium mixed fertilizer and the carrier with the organic fertilizer and a plant insecticide; spraying the amino acid chelated micro or medium fertilizer solution; granulating; and finally coating with the propanediol sebacate polyester. According to the special fertilizer, the utilization rate of fertilizer efficiency can be remarkably increased, the slow release effect is achieved, the fertilizing amount is reduced, and the pesticide residues are reduced, so that the yield and the quality of the rice are improved.