Bacteriophage with environment disinfection capability and applications thereof

Abstract

The invention belongs to the field of bioengineering, and provides a bacteriophage with environment disinfection capability and applications thereof. The bacteriophage monomer is escherichia coli bacteriophage, has a latin name of E.coli phage, and is named Bp7, and has broad-spectrum bactericidal capability to escherichia coli, the bacteriaophage Bp7 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on 6th, June, 2012 and has a preservation number of CGMCC No.6201; the bacteriophage has strong cracking effect on escherichia coli, provides a bacteriophage source for industrial production of bacteriophage and can be used for disinfection; and the bacteriophage can also be used as a safe biological disinfectant for environment disinfection, thus controlling aquaculture ppollution and laboratory pollution; the bacteriophage can be used for industrialization production, can be amplified by specificity of host bacteria Escherichia coli, and can be used as a biological disinfectant for laboratory environment disinfection and chicken house environment disinfection.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a bacteriophage strain capable of disinfecting the environment and its application. Background technique [0002] Phage is a type of virus that infects bacteria. It is often isolated from natural environmental samples such as sewage or feces, and it has strict host specificity, only invading one or a few bacteria, so phage is not infectious to humans, animals and plants , It will not pollute the environment and has high safety. Phages can be divided into two categories: temperate phages and virulent phages, among which virulent phages can lyse sensitive bacteria in a short time. In terms of bactericidal effect, virulent phage can lyse bacteria and have exponential proliferation characteristics, and can be used as a biological disinfectant with antibacterial effect. [0003] The pollution caused by the large-scale breeding of livestock and poultry has always been a seri...

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Problems solved by technology

[0005] The present invention provides a bacteriophage with the ability to disinfect the environment and its use, aiming to solve the serious problem in the breeding industry of large-scale breeding of livestock and poultry. It is not only the main source of pollution in rural areas of our country, but also caused The outbreak of the epidemic also caused huge economic losses to the farmers, and seriously affected my country's social economy, public safety, and international trade in animals and animal products. In order to control pollution, various chemical disinfectants are often used frequently and in large quantities in the farms. However, chemical disinfectants will not only cause harm to the animal body, cause disease, disability, and death of livestock and poultry production accidents, but also cause harm to farm staff, causing allergic reactions and even poisoning symptoms, and domestic researchers have done Many similar bacteriophage disinfection tests have been conducted, but only phage simulation disinfection tests were carried out under laboratory conditions. The phage isolates were isolated from hospital sewage, not from the chicken farm environment, and no disinfection tests on the field environment of phage chicken farms were carried out. question

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