Chinese herb extract with antibacterial effect and preparation method for Chinese herb extract

A technology of Chinese herbal extracts and antibacterial effects, applied in antibacterial drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as increasing the supply of animal products, environmental pollution, and heavy metal residues in animal feces.

Active Publication Date: 2015-11-18
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the problem of drug resistance caused by the abuse of antibiotics in the farming industry cannot be ignored.
Moreover, the long-term and unscientific use of antibiotics in large quantities will also cause heavy metal residues in animal feces, leading to environmental pollution and serious harm to human health. The rapid development of the breeding industry has not only increased the supply of animal products, but also brought food safety. The problem

Method used

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  • Chinese herb extract with antibacterial effect and preparation method for Chinese herb extract
  • Chinese herb extract with antibacterial effect and preparation method for Chinese herb extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1 has the preparation of the Chinese herbal medicine extract of antibacterial effect

[0009] Recipe: 120-180 parts of Polygonum, 120-180 parts of Patchouli, 120-180 parts of Ligustrum lucidum, and 80-120 parts of Astragalus. The specific formula of each sample is shown in Table 1.

[0010] Table 1 has the formula table of the Chinese herbal medicine extract of antibacterial effect

[0011] Sample formula serial number

[0012] Preparation method: mix all the raw materials and extract them with water decoction and alcohol extraction. The water decoction and alcohol extraction are carried out according to the following operations: Weigh 20g of each raw material by weight, pulverize, mix, add 10 times of water and decoct 3 times, Water temperature 100°C, 1.5 hours each time; filter, combine the filtrates for 3 times and centrifuge at 3000r / min for 30min to collect the supernatant; use a rotary evaporator to concentrate the supernatant to a small volu...

Embodiment 2

[0013] The preparation of embodiment 2 control samples

[0014] Water extract control

[0015] Weigh 20 g of each raw material according to formula 4, pulverize, mix, add 10 times of water and decoct 3 times at 100°C for 1.5 hours each time; filter, combine the filtrates 3 times, centrifuge at 3000r / min for 30min to collect the supernatant, The supernatant was concentrated with a rotary evaporator and the volume was adjusted to 20ml, and then stored at 4°C for future use.

[0016] Microwave Extract Control

[0017] Weigh 20 g of each raw material according to formula 4, pulverize, mix, add 10 times of water and mix thoroughly, and place in a microwave sample processing system at 100°C for 15 min.

[0018] Ethanol graded extract control

[0019] Weigh 20 g of each raw material according to formula 4, pulverize, mix, add 10 times volume of 95%, 70%, 45% ethanol to extract 3 times, and the extraction time is 48h, 24h, 24h.

Embodiment 3

[0020] Embodiment 3 has the application pressure test of the Chinese herbal medicine extract of antibacterial effect

[0021] 1. Test material

[0022] Get the sample of formula 4 among the embodiment 1 (hereinafter referred to as the decocted ethanol extract of compound Polygonum polygonum or decocted ethanol extract) and the control sample among the embodiment 2 for following test.

[0023] 2. Determination of antibacterial effect in vitro

[0024] Preparation of bacteria solution: fully recover test bacteria such as Salmonella pullorum, Escherichia coli, Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa and inoculate them on nutrient agar plates, culture them at 37°C for 24 hours, pick typical colonies for inoculation In the nutrient broth medium, culture at 37°C for 18h, adjust the bacterial concentration to 3×10 8 A / mL spare.

[0025] Determination method: The in vitro antibacterial effect of each extract was determined by the disc method, and penicill...

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Abstract

The invention discloses a Chinese herb extract with an antibacterial effect. The Chinese herb extract is prepared by decocting the following raw materials in parts by weight with water and performing alcohol extraction: 120-180 parts of ciderage, 120-180 parts of pogostemon cablin, 120-180 parts of glossy privet fruit and 80-120 parts of astragalus membranaceus. An in-vitro antibacterial test result shows that the Chinese herb extract disclosed by the invention is good in antibacterial effect and has broad spectrum activity; compared with a water extract, a microwave extract and an ethanol fractionated extract, the Chinese herb extract is better in antibacterial effect. The Chinese herb extract is used as an antibacterial medicament for poultry substitute antibiotics and chemical medicaments, so that pollution, caused by drug resistance and drug retaining, to a poultry product can be effectively avoided; the poultry harmful excrements, the protein use amount and the nitrogen pollution are reduced; the Chinese herb extract has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of antibacterial drugs, and in particular relates to an antibacterial Chinese herbal medicine extract and a preparation method thereof. Background technique [0002] With the rapid development of the breeding industry, antibiotics are used more and more widely, and they have played a certain positive role in the prevention and treatment of animal diseases. However, in recent years, the problem of drug resistance caused by the abuse of antibiotics in aquaculture cannot be ignored. Moreover, the long-term and unscientific use of antibiotics in large quantities will also cause heavy metal residues in animal feces, leading to environmental pollution and serious harm to human health. The rapid development of the breeding industry has not only increased the supply of animal products, but also brought food safety. The problem. Broad-spectrum, low-toxicity, and no-residue traditional Chinese medicine compound is m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/704A61P31/04
Inventor 胡庭俊陶俊宇谭红连李璐韦英益曾芸
Owner GUANGXI UNIV
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