The invention discloses a stereotaxic localization rotating disc type multi-project dry-type fluorescence detector. The stereotaxic localization rotating disc type multi-project dry-type fluorescence detector comprises a mounting plate, a testing card bearing plate, a card pulling device, a card entry device, a card reading detection device, a card exit device, a card pressing device and a card clamping device, wherein the mounting plate is fixedly arranged, the testing card bearing plate is fixedly arranged under the mounting plate, the card pulling device is rotationally overlapped on the testing card bearing plate, a card groove for preventing a testing card from sliding is formed in the testing card bearing plate, the testing card is radially conveyed to the clamping groove by the card entry device, the card reading detection device moves radially to detect the testing card, a baffle plate for preventing the testing card from sliding out of the card groove is arranged on the testing card bearing plate, the card pressing device can press the testing card when the card reading detection device performs detection, the card clamping device clamps the testing card from two sides and rights the testing card before the card reading detection device performs detection, and the testing card can be conveyed out of the card groove by the card exit device. According to the stereotaxic localization rotating disc type multi-project dry-type fluorescence detector, the detection error can be avoided, and the reliability and the stability of the detector are improved.