103results about How to "Good sensitivity" patented technology

Reagent box for simultaneously detecting diversified components of animal origin and application of reagent box

The invention discloses a reagent box for simultaneously detecting diversified components of animal origin and application of the reagent box. Fluorescent primer multiplex amplification and capillary electrophoresis methods are combined with one another, so that the 12 components of animal origin can be simultaneously detected by the aid of the reagent box, and a plurality of groups of specific primers can be designed by the aid of Escherichia coli and selected STR (short tandem repeat) sequences of dogs, cattle, sheep, fish, pigs, ducks, chickens, rabbits, cats, horses and rats. The specific primers include specific primers PEZ1 of the dogs, specific primers BM2113 of the cattle, specific primers MAF33 of the sheep, specific primers HLJ392 of the fish, specific primers SW742 of the pigs, specific primers CAUD056 of the ducks, specific primers MCW248 of the chickens, specific primers Sat7 of the rabbits, specific primers FCA955 of the cats, specific primers HMS3 of the horses, specific primers cds of the rats and specific primers CFS073 of the Escherichia coli. The reagent box and the application have the advantages that the reagent box is good in specificity and high in sensitivity, and origin of meat can be quickly determined; multiplex amplification systems contain the primers of the Escherichia coli, and accordingly whether meet foods and products are contaminated by Escherichia coli or not can be simultaneously determined.

Colloidal gold immunochromatography test strip for detecting bovine rotavirus, and preparation method and application thereof

The invention discloses a colloidal gold immunochromatography test strip for detecting a bovine rotavirus, and a preparation method and an application thereof. The test strip includes a sample pad, acolloidal gold pad, a nitrocellulose membrane and an absorbent filter paper which are sequentially connected from left to right, and also includes a PVC base plate positioned at the bottom, wherein the PVC base plate is used to provide an assembling platform, the nitrocellulose membrane is provided with a quality control line formed by spraying a goat anti-mouse IgG and a detection line formed byspraying a rabbit anti-bovine rotavirus polyclonal antibody, the colloidal gold pad is coated with a colloidal gold particle-labeled mouse anti-bovine rotavirus VP6 protein monoclonal antibody, and the sample pad provides a position for adding a sample to be detected, wherein the mouse anti-bovine rotavirus VP6 protein monoclonal antibody is secreted by a hybridoma cell strain preserved in China General Microbiological Culture Collection Center with the preservation number being CGMCC NO.14726. The test strip prepared in the invention has the advantages of good sensitivity, high specificity, simplicity and rapidness in operation, and suitableness for onsite detection.

Tracing method of biological pollution of water body

The invention relates to the field of water quality detection, and in particular relates to a tracing method of biological pollution of a water body. The method comprises the following steps: determining characteristic microorganisms in target water body pollutants; selecting specific gene fragments of the characteristic microorganisms; and quantitating pollution sources and pollution conditions of the target water body through the specific gene fragments. According to the method provided by the invention, the characteristic microorganisms in the target water body pollutants are determined; then the pollution sources and the pollution conditions of the target water body are quantitated through the specific gene fragments of the characteristic microorganisms, so that the detection of a pollution level is improved to a molecular level. Thus, the high accuracy and the good sensitivity are realized; the target pollution sources are positioned well. Therefore, the sources of feces are accurately positioned and the pollution contribution rate of the water body is quantitated. As a result, a certain guiding significance in the objective evaluation of the aquaculture pollution contribution rate and the treatment of the pollution of the water body in a manner of adjusting measures to local conditions is provided.

Method for detecting EDTA (ethylene diamine tetraacetic acid) in laundry detergent

The invention relates to a method for detecting EDTA (ethylene diamine tetraacetic acid) in a laundry detergent. The method comprises steps as follows: (1) preparing a potassium bicarbonate aqueous solution; (2) adding the to-be-detected laundry detergent to the potassium bicarbonate aqueous solution, stirring the solution at the temperature of 45-50 DEG C for 10-20 min, filtering the solution while the solution is hot, and naturally cooling the solution to the room temperature to obtain a pretreated solution; (3) adding an iron liquid to the pretreated solution, performing oscillating extraction and then centrifugation, and removing an aqueous phase to obtain an ion liquid phase; (4) adding trivalent iron salt to the ion liquid phase, after full and even mixing, performing chromatography separation with a gel column chromatography Superose 12 column, performing washing with n-hexane firstly, then performing gradient elution with a petroleum ether-normal propyl alcohol mixed solvent serving as an eluent, and collecting the eluent; (5) evaporation the solvent of the eluent until the solvent is almost dry, and performing dissolution, volume determination and filtration with an organic membrane to obtain a detection sample; (6) performing liquid chromatography separation on the to-be-detected sample to determine the content. The method is excellent in EDTA quantitation accuracy, and a new detection means is provided for control on the content of the EDTA in the laundry detergent.

Preparation of two antibody and antigen conjugates and method thereof for simultaneously detecting alpha-fetoprotein and carcinoembryonic antigen

The invention relates to the field of medical tumor marker detection, in particular to preparation of two antibody and antigen conjugates respectively from 1,1'-ferrocenediacetic acid/gold@platinum and thionine/gold@ platinum and a method thereof for simultaneously performing specificity detection on alpha-fetoprotein and carcinoembryonic antigen. The 1,1'-ferrocenediacetic acid/gold@platinum and thionine/gold@ platinum are respectively used for preparing the two kinds of antibody and antigen conjugates; according to the method for simultaneously detecting the alpha-fetoprotein AFP and the carcinoembryonic antigen CEA by an electrochemical sensor to be built, a modified glassy carbon electrode is put into a mixed solution of the alpha-fetoprotein and the carcinoembryonic antigen; then, active sites which are not subjected to specific binding are sealed; after the glassy carbon electrode is taken out, alpha-fetoprotein antigen and carcinoembryonic antigen mixtures with different concentrations are sequentially used for modifying the glassy carbon electrode, so that the antigen and the antibody realize the specific bonding; a sandwich immunosensor is built; the sandwich immunosensor is used for detecting the concentration of the alpha-fetoprotein and carcinoembryonic antigen by using a DPV (differential pulse voltammetry) method.
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products