Tracing method of biological pollution of water body

A technology for water organisms and water pollutants, used in biochemical equipment and methods, and the determination/inspection of microorganisms. It can solve problems such as pollution, water blackening, human and animal health hazards, and achieve good sensitivity and high accuracy. Effect

Inactive Publication Date: 2014-09-03
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Description
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  • Application Information

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Problems solved by technology

The aquaculture industry is gradually developing towards large-scale intensification, and large-scale aquaculture produces a lot of manure. It is difficult for the traditional manure treatment methods to meet such a large manure treatment requirement.
When the total amount of feces discharged into the water body exceeds the natural purification capacity of the water body, it will cause pollution; in addition, the pathogenic microorganisms brought into the water body by the feces will also cause harm to the health of people and animals; excessive feces pollution will be accompanied by surface runoff and Surface water and groundwater are polluted by underground seepage into nearby water bodies; fecal pollution will lead to a significant increase in suspended solids, chemical oxygen demand, and biological oxygen demand in water bodies after fecal pollution enters water bodies with surface runoff; microorganisms in water bodies can d

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  • Tracing method of biological pollution of water body
  • Tracing method of biological pollution of water body
  • Tracing method of biological pollution of water body

Examples

Experimental program
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Example Embodiment

[0044] Example 1

[0045] Use sterilized sampling bottles to collect pig farm manure samples, cow manure, chicken manure, and human manure samples, store them at low temperature, and transport the samples back to the laboratory for processing within 24 hours.

[0046] Use a 0.22μm microporous filter membrane to filter a certain amount of pig farm wastewater, grind the filtered membrane with liquid nitrogen, and transfer the ground powder to a 1.5ml centrifuge tube; take a certain quality of fecal sample and grind it with liquid nitrogen , Transfer the ground powder to a 1.5ml centrifuge tube; use the modified CTAB method to extract DNA, the steps are as follows:

[0047] Add 1ml TENP buffer (50mmol / L Tris-HCl, 20mmol / L EDTA, 100mmol / L NaCl, 0.01g / ml PVP, pH=10) into a 1.5ml centrifuge tube, vortex and mix well, centrifuge at 10000r / min for 10min , Discard the supernatant, repeat the above steps until the supernatant is clear and transparent, leave the pellet, add 500μL of 2×CTAB ext...

Example Embodiment

[0078] Example 2

[0079] Pig farm wastewater is rich in nitrogen, phosphorus and organic matter, and these pollutants are the main cause of eutrophication of water bodies. Studying the correlation between microorganisms and pollutants in water bodies is a prerequisite for using microorganisms to trace the source of nitrogen and phosphorus organic matter in water bodies. Taking the environmentally controllable micro system as the research object, studying the changes of total nitrogen, total phosphorus and chemical oxygen demand of pig farm wastewater under different temperature treatments, and determining the copy number of pig-origin host Bacteroides biomarker Pig-2-Bac The law of change is to quantify the degree of water eutrophication by measuring the copy number of specific gene fragments.

[0080] 1. The effect of temperature on the stability of pig-derived Bacteroides specific biomarker Pig-2-Bac. Remove the suspended solids in the wastewater from a large-scale pig farm in...

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Abstract

The invention relates to the field of water quality detection, and in particular relates to a tracing method of biological pollution of a water body. The method comprises the following steps: determining characteristic microorganisms in target water body pollutants; selecting specific gene fragments of the characteristic microorganisms; and quantitating pollution sources and pollution conditions of the target water body through the specific gene fragments. According to the method provided by the invention, the characteristic microorganisms in the target water body pollutants are determined; then the pollution sources and the pollution conditions of the target water body are quantitated through the specific gene fragments of the characteristic microorganisms, so that the detection of a pollution level is improved to a molecular level. Thus, the high accuracy and the good sensitivity are realized; the target pollution sources are positioned well. Therefore, the sources of feces are accurately positioned and the pollution contribution rate of the water body is quantitated. As a result, a certain guiding significance in the objective evaluation of the aquaculture pollution contribution rate and the treatment of the pollution of the water body in a manner of adjusting measures to local conditions is provided.

Description

technical field [0001] The invention relates to the field of water quality detection, in particular to a method for tracing the source of water body biological pollution. Background technique [0002] Water is the source of life, and the quality of water directly affects human production and life. In recent years, with the improvement of people's quality of life and the increase in demand for animal husbandry products, my country's livestock and poultry breeding industry has developed rapidly. The aquaculture industry is gradually developing towards scale and intensification, and large-scale aquaculture produces a lot of manure, and the traditional manure treatment methods have been difficult to meet such a large manure treatment requirement. When the total amount of feces discharged into the water body exceeds the natural purification capacity of the water body, it will cause pollution; in addition, the pathogenic microorganisms brought into the water body by the feces wil...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/06C12Q1/04
CPCC12Q1/6851C12Q2531/113C12Q2561/113
Inventor 郭萍朱昌雄王显贵田云龙张莉
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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