Tracing method of biological pollution of water body

Abstract

The invention relates to the field of water quality detection, and in particular relates to a tracing method of biological pollution of a water body. The method comprises the following steps: determining characteristic microorganisms in target water body pollutants; selecting specific gene fragments of the characteristic microorganisms; and quantitating pollution sources and pollution conditions of the target water body through the specific gene fragments. According to the method provided by the invention, the characteristic microorganisms in the target water body pollutants are determined; then the pollution sources and the pollution conditions of the target water body are quantitated through the specific gene fragments of the characteristic microorganisms, so that the detection of a pollution level is improved to a molecular level. Thus, the high accuracy and the good sensitivity are realized; the target pollution sources are positioned well. Therefore, the sources of feces are accurately positioned and the pollution contribution rate of the water body is quantitated. As a result, a certain guiding significance in the objective evaluation of the aquaculture pollution contribution rate and the treatment of the pollution of the water body in a manner of adjusting measures to local conditions is provided.

Description

technical field [0001] The invention relates to the field of water quality detection, in particular to a method for tracing the source of water body biological pollution. Background technique [0002] Water is the source of life, and the quality of water directly affects human production and life. In recent years, with the improvement of people's quality of life and the increase in demand for animal husbandry products, my country's livestock and poultry breeding industry has developed rapidly. The aquaculture industry is gradually developing towards scale and intensification, and large-scale aquaculture produces a lot of manure, and the traditional manure treatment methods have been difficult to meet such a large manure treatment requirement. When the total amount of feces discharged into the water body exceeds the natural purification capacity of the water body, it will cause pollution; in addition, the pathogenic microorganisms brought into the water body by the feces wil...

AI Technical Summary

Problems solved by technology

The aquaculture industry is gradually developing towards large-scale intensification, and large-scale aquaculture produces a lot of manure. It is difficult for the traditional manure treatment methods to meet such a large manure treatment requirement.

When the total amount of feces discharged into the water body exceeds the natural purification capacity of the water body, it will cause pollution; in addition, the pathogenic microorganisms brought into the water body by the feces will also cause harm to the health of people and animals; excessive feces pollution will be accompanied by surface runoff and Surface water and groundwater are polluted by underground seepage into nearby water bodies; fecal pollution will lead to a significant increase in suspended solids, chemical oxygen demand, and biological oxygen demand in water bodies after fecal pollution enters water bodies with surface runoff; microorganisms in water bodies can degrade organic matter The production of a large amount of nitrogen and phosphorus provides excess nutrients for the growth of aquatic plants, increasing the risk of eutrophication of water bodies; generally, in water bodies seriously polluted by aquaculture wastewater, its dissolved oxygen is consumed in large quantities, and aquatic organisms die due to hypoxia and suffocation, eventually Causes water to turn black and stink

[0003] At present, the pollution contribution rate of the aquaculture industry is based on the theoretical value calculated by the pollution coefficient. The consideration is based on the theoretical value of the pollution coefficient, which is different from the actual pollution contribution rate. Most of the current treatment work stays at the terminal treatment stage of pollution control, which increases the cost of pollution control to a certain extent (Stoner N et al., 2007; Feng Guangda et al., 2010)

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