Use method of Wright-Giemsa dye liquor

A technology of Wright's and dyeing solution, applied in the field of medical reagents, can solve the problems of poor dyeing effect and long dyeing time of vaginal secretions

Pending Publication Date: 2021-01-29
URIT MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for using Wright-Giemsa dye solution, aiming to solve the tec

Method used

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  • Use method of Wright-Giemsa dye liquor
  • Use method of Wright-Giemsa dye liquor
  • Use method of Wright-Giemsa dye liquor

Examples

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Example Embodiment

[0031]Example 1, please refer tofigure 1 , The present invention provides a method for using Wright-Giemsa dye solution, which includes the following steps:

[0032]S1: Mix the dye solution A and dye solution B in a quantitative ratio to obtain a mixed dye;

[0033]S2: Spread the vaginal secretions evenly on the glass slide, and put the glass slide coated with the vaginal secretions in the oven, and bake them at 40°C to complete curing;

[0034]S3: Set the slide glass at an angle of 20°, and then drop the mixed dye onto the cured vaginal secretions for staining. The staining time is 20s, and then rinse with purified water;

[0035]S4: Dry the cleaned vaginal secretions, and then perform microscopic examination.

[0036]Wherein, the dye solution A is a Wright dye solution, and the dye solution B is a Giemsa dye solution. In the step of mixing the dye solution A and the dye solution B in a quantitative ratio to obtain the mixed dye, the dye solution A and the dyeing solution B are stirred and mixed ...

Example Embodiment

[0037]Example 2, please refer tofigure 2 , The present invention provides a method for using Wright-Giemsa dye solution, which includes the following steps:

[0038]S1: Mix the dye solution A and dye solution B in a quantitative ratio to obtain a mixed dye;

[0039]S2: Spread the vaginal secretions evenly on the glass slide, and put the glass slide coated with the vaginal secretions into the oven, and bake them at 60°C to complete the curing;

[0040]S3: Set the slide glass at an angle of 40°, and then drop the mixed dye onto the cured vaginal secretions for dyeing, the dyeing time is 40s, and then rinse with purified water;

[0041]S4: Dry the cleaned vaginal secretions, and then perform microscopic examination.

[0042]Wherein, the dye solution A is a Wright dye solution, and the dye solution B is a Giemsa dye solution. In the step of mixing the dye solution A and the dye solution B in a quantitative ratio to obtain the mixed dye, the dye solution A and the dyeing solution B are stirred and mixe...

Example Embodiment

[0043]Example 3, please refer toimage 3 , The present invention provides a method for using Wright-Giemsa dye solution, which includes the following steps:

[0044]S1: Mix the dye solution A and dye solution B in a quantitative ratio to obtain a mixed dye;

[0045]S2: Spread the vaginal secretions evenly on the glass slide, and put the glass slide coated with the vaginal secretions in the oven, and bake them at 50°C to complete curing;

[0046]S3: Set the slide glass at an angle of 30°, and then drop the mixed dye onto the cured vaginal secretions for staining, the staining time is 30 seconds, and then rinse with purified water;

[0047]S4: Dry the cleaned vaginal secretions, and then perform microscopic examination.

[0048]Wherein, the dye solution A is a Wright dye solution, and the dye solution B is a Giemsa dye solution. In the step of mixing the dye solution A and the dye solution B in a quantitative ratio to obtain the mixed dye, the dye solution A and the dyeing solution B are stirred and ...

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Abstract

The invention discloses a use method of a Wright's Giemsa staining solution, and the method comprises the following steps: uniformly mixing a staining solution A and a staining solution B, uniformly coating vaginal secretion on a glass slide, and curing the vaginal secretion on the glass slide; dyeing the vaginal secretion, so that visible components in the vaginal secretion are dyed. According tothe dyeing method, the color depth of the cells is suitable, the observation and the judgment are easily achieved, the bottom color is light, the vaginal secretion is basically colorless, the forms of various cells and microorganisms are clearly presented, and the misjudgment on the visible components cannot be caused; particularly, the dyeing effect of fine particles such as fungi and bacteria is clear and visible, and the dyeing time is greatly shortened.

Description

technical field [0001] The invention relates to the technical field of medical reagents, in particular to a method for using Wright-Giemsa stain. Background technique [0002] Vaginal secretion examination is one of the routine items of clinical examination in gynecology and obstetrics of modern medicine, and plays a very important role in the diagnosis of diseases. For example, in the inspection and analysis system for the formed components of vaginal secretions, the detection rate of pathogenic causes can be improved by using the staining method. In the traditional Wright-Giemsa staining method, after staining with staining solution A for 2 minutes, add staining solution B, blow and mix evenly, and then dye for 2 minutes. The effect is poor and the dyeing time is long. Contents of the invention [0003] The purpose of the present invention is to provide a method for using Wright-Giemsa dye solution, aiming to solve the technical problems that the dyeing method in the p...

Claims

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Application Information

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IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 赵丽珍刘云鹏龙锦婕
Owner URIT MEDICAL ELECTRONICS CO LTD
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