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Use method of Wright-Giemsa dye liquor

A technology of Wright's and dyeing solution, applied in the field of medical reagents, can solve the problems of poor dyeing effect and long dyeing time of vaginal secretions

Pending Publication Date: 2021-01-29
URIT MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for using Wright-Giemsa dye solution, aiming to solve the technical problems that the dyeing method in the prior art has poor dyeing effect on vaginal secretions and the dyeing time is long

Method used

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  • Use method of Wright-Giemsa dye liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1, see figure 1 , the invention provides a method for using Wright-Giemsa stain, comprising the steps of:

[0032] S1: Mix the dyeing solution A and the dyeing solution B according to a quantitative ratio to obtain a mixed dye;

[0033] S2: Spread the vaginal secretion evenly on the glass slide, put the glass slide coated with the vaginal secretion evenly in an oven, and dry it at 40°C to complete the curing;

[0034] S3: Set the slide glass at an inclination of 20°, then drop the mixed dye onto the cured vaginal secretion for staining, the staining time is 20s, and then wash with purified water;

[0035] S4: Dry and wash the vaginal secretions, and then perform a microscopic examination.

[0036] Wherein, the staining solution A is Wright's staining solution, and the staining solution B is Giemsa's staining solution. In the step of mixing the staining solution A and the staining solution B in a quantitative ratio to obtain the mixed dyeing agent, the staining...

Embodiment 2

[0037] Example 2, see figure 2 , the invention provides a method for using Wright-Giemsa stain, comprising the steps of:

[0038] S1: Mix the dyeing solution A and the dyeing solution B according to a quantitative ratio to obtain a mixed dye;

[0039] S2: Spread the vaginal secretion evenly on the glass slide, put the glass slide coated with the vaginal secretion evenly in an oven, and dry it at 60°C to complete the curing;

[0040] S3: set the slide glass at an inclination of 40°, and then drop the mixed dye onto the cured vaginal secretion for staining. The staining time is 40 seconds, and then wash with purified water;

[0041] S4: Dry and wash the vaginal secretions, and then perform a microscopic examination.

[0042] Wherein, the staining solution A is Wright's staining solution, and the staining solution B is Giemsa's staining solution. In the step of mixing the staining solution A and the staining solution B in a quantitative ratio to obtain the mixed dyeing agent, ...

Embodiment 3

[0043] Example 3, see image 3 , the invention provides a method for using Wright-Giemsa stain, comprising the steps of:

[0044] S1: Mix the dyeing solution A and the dyeing solution B according to a quantitative ratio to obtain a mixed dye;

[0045] S2: Spread the vaginal secretion evenly on the glass slide, put the glass slide coated with the vaginal secretion evenly in an oven, and dry it at 50°C to complete the curing;

[0046] S3: set the slide glass at an inclination of 30°, then drop the mixed dye onto the cured vaginal secretion for staining, the staining time is 30s, and then wash with purified water;

[0047] S4: Dry and wash the vaginal secretions, and then perform a microscopic examination.

[0048] Wherein, the staining solution A is Wright's staining solution, and the staining solution B is Giemsa's staining solution. In the step of mixing the staining solution A and the staining solution B in a quantitative ratio to obtain the mixed dyeing agent, the staining s...

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Abstract

The invention discloses a use method of a Wright's Giemsa staining solution, and the method comprises the following steps: uniformly mixing a staining solution A and a staining solution B, uniformly coating vaginal secretion on a glass slide, and curing the vaginal secretion on the glass slide; dyeing the vaginal secretion, so that visible components in the vaginal secretion are dyed. According tothe dyeing method, the color depth of the cells is suitable, the observation and the judgment are easily achieved, the bottom color is light, the vaginal secretion is basically colorless, the forms of various cells and microorganisms are clearly presented, and the misjudgment on the visible components cannot be caused; particularly, the dyeing effect of fine particles such as fungi and bacteria is clear and visible, and the dyeing time is greatly shortened.

Description

technical field [0001] The invention relates to the technical field of medical reagents, in particular to a method for using Wright-Giemsa stain. Background technique [0002] Vaginal secretion examination is one of the routine items of clinical examination in gynecology and obstetrics of modern medicine, and plays a very important role in the diagnosis of diseases. For example, in the inspection and analysis system for the formed components of vaginal secretions, the detection rate of pathogenic causes can be improved by using the staining method. In the traditional Wright-Giemsa staining method, after staining with staining solution A for 2 minutes, add staining solution B, blow and mix evenly, and then dye for 2 minutes. The effect is poor and the dyeing time is long. Contents of the invention [0003] The purpose of the present invention is to provide a method for using Wright-Giemsa dye solution, aiming to solve the technical problems that the dyeing method in the p...

Claims

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Application Information

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IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 赵丽珍刘云鹏龙锦婕
Owner URIT MEDICAL ELECTRONICS CO LTD
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