Preparation method and dyeing method of vaginal secretion dyeing liquid

A technology of vaginal secretions and preparation methods, which is applied in the field of medical reagents, can solve the problems of poor dyeing effect and long dyeing time, and achieve good dyeing effect, appropriate coloring depth, and facilitate observation and judgment

Pending Publication Date: 2021-01-29
URIT MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a preparation method and a dyeing method of a vaginal secretion dyeing solution, aiming to solve the technical problems that the dyeing solution in the prior art has a poor dyeing effect on vaginal secretions and the dyeing time is long

Method used

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  • Preparation method and dyeing method of vaginal secretion dyeing liquid
  • Preparation method and dyeing method of vaginal secretion dyeing liquid
  • Preparation method and dyeing method of vaginal secretion dyeing liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1, see figure 1 , the invention provides a kind of preparation method of vaginal secretion dyeing liquid, comprises the steps:

[0036] S1: In parts by weight, mix 1 part of Swiss dye powder, 1 part of Giemsa dye solution, 15 parts of phosphate buffer saline, 45 parts of methanol, 2 parts of glycerin and 0.01 part of SDS at a speed of 100 r / min, and the stirring time is 1min, mixed uniformly under ambient conditions of 22°C to obtain a mixture;

[0037] S2: The mixture is sealed and stored, and the mixture is left to stand for 38 hours to obtain a staining solution.

[0038] Wherein, in the step of sealing and storing the mixture, and standing the mixture for 38 hours to obtain the dyeing solution: after sealing and storing the mixture, ultrasonication is used for 1-3 hours, and the mixture is left standing in an environment of 5-40°C .

[0039] By weighing 1 part of Swiss dye powder, 1 part of Giemsa dye solution, 15 parts of phosphate buffer saline, 45 part...

Embodiment 2

[0040] Example 2, see figure 2 , the invention provides a kind of preparation method of vaginal secretion staining liquid, comprises the steps:

[0041] S1: In parts by weight, 2.5 parts of Swiss dye powder, 1.5 parts of Giemsa dye solution, 17.5 parts of phosphate buffer, 50 parts of methanol, 3.5 parts of glycerol and 0.02 parts of SDS were mixed at a speed of 200r / min, and the stirring time was 1.5min, mixed uniformly under the ambient condition of 25°C to obtain the mixture;

[0042] S2: seal and store the mixture, and let the mixture stand for 48 hours to obtain a staining solution.

[0043] Wherein, in the step of sealing and storing the mixture, and standing the mixture for 48 hours to obtain the dyeing solution: after sealing and storing the mixture, ultrasonic waves are used for 1-3 hours, and the mixture is left standing in an environment of 5-40°C .

[0044]By weighing 2.5 parts of Swiss dye powder, 1.5 parts of Giemsa dye solution, 17.5 parts of phosphate buffe...

Embodiment 3

[0045] Example 3, see image 3 , the invention provides a kind of preparation method of vaginal secretion dyeing liquid, comprises the steps:

[0046] S1: In parts by weight, mix 4 parts of Swiss dye powder, 2 parts of Giemsa dye solution, 20 parts of phosphate buffer saline, 60 parts of methanol, 5 parts of glycerin and 0.03 part of SDS at a speed of 300r / min, and the stirring time is 2min, mixed uniformly under ambient conditions of 28°C to obtain a mixture;

[0047] S2: The mixture is sealed and stored, and the mixture is left to stand for 58 hours to obtain a staining solution.

[0048] Wherein, in the step of sealing and storing the mixture, and standing the mixture for 58 hours to obtain the dyeing solution: after sealing and storing the mixture, ultrasonic waves are used for 1-3 hours, and the mixture is left standing in an environment of 5-40°C .

[0049] By weighing 4 parts of Swiss dye powder, 2 parts of Giemsa dye solution, 20 parts of phosphate buffer saline, 60...

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Abstract

The invention discloses a preparation method and dyeing method of vaginal secretion dyeing liquid, and the method comprises the following steps: preparing dyeing liquid formed by mixing Swiss dyeing powder, a Giemsa dyeing liquid, a phosphate buffer solution, methanol, glycerol and SDS, uniformly coating a sample on a glass slide, and curing the sample on the glass slide; dripping the prepared dyeing liquid on the cured sample for dyeing, and performing washing; and completing the dyeing of the aired and washed sample. According to the invention, the prepared staining solution is good in staining effect, proper in cell staining depth of the sample, beneficial to observation and judgment, light in bottom color, basically colorless, free of misjudgment on visible components, and capable of greatly shortening the staining time of vaginal secretion by only one-step staining.

Description

technical field [0001] The invention relates to the technical field of medical reagents, in particular to a preparation method and a dyeing method of a vaginal secretion dyeing solution. Background technique [0002] Vaginal secretion examination is one of the routine items of clinical examination in gynecology and obstetrics of modern medicine, and plays a very important role in the diagnosis of diseases. For example, in the inspection and analysis system for the formed components of vaginal secretions, the detection rate of pathogenic causes can be improved by using the staining method. However, the existing dyeing solution has poor dyeing effect on vaginal secretions, and the dyeing time is long. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method and a dyeing method of a vaginal secretion dyeing solution, aiming to solve the technical problems that the dyeing solution in the prior art has poor dyeing effect on vag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31
CPCG01N1/30G01N1/31G01N2001/302
Inventor 赵丽珍刘云鹏龙锦婕
Owner URIT MEDICAL ELECTRONICS CO LTD
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