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Hematoxylin counterstain solution and staining method for immunohistochemical staining

A technique of immunohistochemistry and staining method, applied in the field of hematoxylin counterstain and staining, and can solve problems such as affecting the observation of positive signals of immunohistochemistry

Active Publication Date: 2021-04-02
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blue-green color is not very clear against the gray-brown background, which affects the observation of positive signals in immunohistochemistry

Method used

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  • Hematoxylin counterstain solution and staining method for immunohistochemical staining
  • Hematoxylin counterstain solution and staining method for immunohistochemical staining
  • Hematoxylin counterstain solution and staining method for immunohistochemical staining

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5 and comparative example 1～3

[0049]Follow the following formulations to formulate Suimin Down:

[0050]

[0051]A slice of clinical samples of a prepared thyroid nodule, esophageal carcinoma and pulmonary swollen, respectively, in accordance with the preferred conditions in the above method, and the conditions are shown in Table 1 below. Because the equidistor of the acidic substance in the nucleus is 3.0 ~ 3.3, theoretically when the pH is less than 3.0, Suikin can nuclear colors. Therefore, the pH values ​​of the retrore solution are set between 2.1 to 2.9, respectively.

[0052]Table 1:

[0053]

[0054]After staining, observe the hue of slices in the slices, and calculate the proportion of normal color (blue purple) slice. The results are shown in Table 2 below.

[0055]Table 2:

[0056] pH Main tone Blue purple proportion Coloration Contrast 1 2.1blue-green 50％full Contrast 2 2.3blue-green 59％full Example 1 2.4Blue-green / blue purple 79％full Example 2 2.5blue purple 96％full Example 3 2.6blue purple 100％full Example 4...

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Abstract

The invention discloses a hematoxylin staining liquid for immumohistochemical staining and a staining method. The hematoxylin staining liquid is a Mayer's hematoxylin staining liquid, wherein the pH value of the hematoxylin staining liquid is 2.4-2.8. The hematoxylin staining liquid disclosed by the invention has a staining effect of a high color contrast ratio after an antigen retrieval step.

Description

Technical field[0001]The present invention relates to the field of immunohistochemical slices, and more particularly to eliminum retrofitting solutions and dyeing methods in immunohardized slice preparation.Background technique[0002]Immunohistochemistry is the "gold standard" technology in pathological tumor test, where the reachable effect of retrorefral solution is critical to the observation of immunohardal signals. Sukin retrofunction is one of the most commonly used retrorefi in immunohistochemistry, which makes the cells to nuclear blue purple by combining acidic substances in the nucleus. The biological tissue is fixed by aldehyde reagents, and the paraffin is buried. During the immunohistochemical dyeing process, most of the primary antigen needs to be tissue antigen repair. The tissue samples were configured with aldehyde groups in the tissue after forming aldehyde reagents, and their antigen decisions were blocked, affecting the binding of antibodies to the antigen to be t...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 蒋小龙范文萍李立科陈其云龙腾骧
Owner SICHUAN MACCURA BIOTECH CO LTD
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