Hematoxylin staining solution, and preparation method thereof

A technology of hematoxylin and staining solution, applied in the field of hematoxylin staining solution and its preparation, can solve the problems of variability of Pap staining solution, complicated dyeing steps, easy crystallization, etc., to avoid hematoxylin precipitation and crystallization, good slime stripping effect, and improve The effect of stable performance

Inactive Publication Date: 2020-02-21
武汉医尔特科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Hematoxylin is a natural dye, which becomes hematoxylin through oxidation. It is usually used as Papanicolaou staining solution and nuclear staining solution for staining the nucleus of biological tissue sections; the preparation of traditional Papanicolaou staining solution is complicated, and the staining steps are complicated. It is easy to crystallize in replacement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The components of the hematoxylin staining solution in this example include: 90ml of isopropanol, 20ml of methanol, 50ml of alcohol, 13ml of acetic acid, 37g of aluminum sulfate, 3.5g of hematoxylin, 0.5g of sodium iodate, 7g of sodium chloride, 3g of disodium hydrogen phosphate, and sodium azide 0.4g, 0.3g of dodecyldimethylbenzyl ammonium bromide, 850ml of water, wherein the purity of isopropyl alcohol is 99%, the purity of methanol is 99.9%, the purity of alcohol is 99.99%, and the purity of acetic acid is 98%. The water is deionized water, and the pH value of the hematoxylin staining solution is 2.0-3.0.

[0024] The preparation method of this example hematoxylin staining solution, its steps are as follows:

[0025] a. Dissolving the hematoxylin of the formula quantity in the alcohol of the formula quantity, stirring evenly to obtain solution a;

[0026] b. Dissolving the aluminum sulfate, sodium chloride and sodium azide of the formula quantity in the deionized wa...

Embodiment 2

[0032] The components of the hematoxylin staining solution in this example include: 90ml of isopropanol, 20ml of methanol, 80ml of alcohol, 13ml of acetic acid, 37g of aluminum sulfate, 3.5g of hematoxylin, 0.5g of sodium iodate, 7g of sodium chloride, 3g of disodium hydrogen phosphate, and sodium azide 0.4g, 0.3g of dodecyldimethylbenzyl ammonium bromide, 750ml of water, wherein the purity of isopropyl alcohol is 99%, the purity of methanol is 99.9%, the purity of alcohol is 99.99%, and the purity of acetic acid is 98%. The water is deionized water, and the pH value of the hematoxylin staining solution is 2.0-3.0.

[0033] The preparation method of this example hematoxylin staining solution, its steps are as follows:

[0034] a. Dissolving the hematoxylin of the formula quantity in the alcohol of the formula quantity, stirring evenly to obtain solution a;

[0035] b. Dissolving the aluminum sulfate, sodium chloride and sodium azide of the formula quantity in the deionized wa...

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PUM

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Abstract

The invention discloses a hematoxylin staining solution. The hematoxylin staining solution comprises the following components: 90ml of isopropanol, 20ml of methanol, 50-80ml of alcohol, 13ml of aceticacid, 37g of aluminum sulfate, 3.5g of hematoxylin, 0.5g of sodium iodate, 7g of sodium chloride, 3g of disodium hydrogen phosphate, 0.4g of sodium azide, 0.3g of dodecyl dimethyl benzyl ammonium bromide, and 750-850ml of water. The invention further provides a preparation method of the hematoxylin staining solution. The preparation method of the hematoxylin staining solution is simple, a staining process is safe, staining operation is simple and convenient, and the staining solution achieves high staining capability and a long preservation cycle.

Description

technical field [0001] The invention relates to the field of biomedical reagents, in particular to a hematoxylin staining solution and a preparation method thereof. Background technique [0002] Papanicolaou staining is the best and most commonly used staining method for pathological sections and exfoliated cells. It is not easy to decolorize and can be stored for a long time. It is often used for staining pathological sections because it can show the different degrees of keratinization of squamous epithelial cells. [0003] Hematoxylin is a natural dye, which becomes hematoxylin through oxidation. It is usually used as Papanicolaou staining solution and nuclear staining solution for staining the nucleus of biological tissue sections; the preparation of traditional Papanicolaou staining solution is complicated, and the staining steps are complicated. It is easy to crystallize in replacement or colder environments, and the dyeing time is longer, making it difficult for inspe...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 刘益民卞忠力徐安双
Owner 武汉医尔特科技有限公司
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