Hematoxylin staining solution, and preparation method thereof

Abstract

The invention discloses a hematoxylin staining solution. The hematoxylin staining solution comprises the following components: 90ml of isopropanol, 20ml of methanol, 50-80ml of alcohol, 13ml of aceticacid, 37g of aluminum sulfate, 3.5g of hematoxylin, 0.5g of sodium iodate, 7g of sodium chloride, 3g of disodium hydrogen phosphate, 0.4g of sodium azide, 0.3g of dodecyl dimethyl benzyl ammonium bromide, and 750-850ml of water. The invention further provides a preparation method of the hematoxylin staining solution. The preparation method of the hematoxylin staining solution is simple, a staining process is safe, staining operation is simple and convenient, and the staining solution achieves high staining capability and a long preservation cycle.

Description

technical field [0001] The invention relates to the field of biomedical reagents, in particular to a hematoxylin staining solution and a preparation method thereof. Background technique [0002] Papanicolaou staining is the best and most commonly used staining method for pathological sections and exfoliated cells. It is not easy to decolorize and can be stored for a long time. It is often used for staining pathological sections because it can show the different degrees of keratinization of squamous epithelial cells. [0003] Hematoxylin is a natural dye, which becomes hematoxylin through oxidation. It is usually used as Papanicolaou staining solution and nuclear staining solution for staining the nucleus of biological tissue sections; the preparation of traditional Papanicolaou staining solution is complicated, and the staining steps are complicated. It is easy to crystallize in replacement or colder environments, and the dyeing time is longer, making it difficult for inspe...

AI Technical Summary

Problems solved by technology

[0003] Hematoxylin is a natural dye, which becomes hematoxylin through oxidation. It is usually used as Papanicolaou staining solution and nuclear staining solution for staining the nucleus of biological tissue sections; the preparation of traditional Papanicolaou staining solution is complicated, and the staining steps are complicated. It is easy to crystallize in replacement or colder environments, and the dyeing time is longer, making it difficult for inspectors to operate and control the dyeing effect, and it is not easy to promote in primary medical units; in addition, the traditional Pap staining solution is volatile and the dyeing effect is unstable , affecting the accuracy of the doctor's pathological reading and the diagnosis of the disease