The invention relates to a novel disc type oxygen sensor and a preparation method and a detection method thereof. The sensor is formed by sequentially superposing an upper protection layer, a signal layer, an air reference channel layer and a heating layer from top to bottom and then sintering, wherein the air reference channel layer comprises a tape casting substrate as the fifth layer, a tape casting substrate as the sixth layer and a tape casting substrate as the seventh layer, and an air reference channel with a closed end is arranged on the surface of the tape casting substrate as the fifth layer and is totally filled with a volatile piece; the preparation method comprises the steps of preparation of a plurality of tape casting slurry, tape casting forming, edge removing and cutting, hole punching, printing, filling, superposition, vacuum sealing, warm isostatic pressing, cutting, sintering and detection. The novel disc type oxygen sensor and the preparation method and the detection method thereof have the advantages that the oxygen sensor is simple in technology and stable in performance, has high adaptability, and can work stably in high temperature.