Mercury-free hematoxylin staining fluid

A technology of hematoxylin and staining solution, which is applied in the field of pathology experiments, can solve problems such as not easy to control, and achieve the effect of clear structure, clear staining and short staining time

Inactive Publication Date: 2016-09-21
北京九州柏林生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mercury oxide used in it is recognized as a highly toxic and harmful chemical, and its configuration method has many precautions, which is not easy to control

Method used

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  • Mercury-free hematoxylin staining fluid
  • Mercury-free hematoxylin staining fluid

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Effect test

Embodiment 1

[0037] In this embodiment, the mercury-free hematoxylin staining solution is composed of a mixed solution and added components, wherein the mixed solution is composed of the following components in parts by volume: 1000 mL of distilled water, 70 mL of glycerol, and 40 mL of 1% periodic acid aqueous solution , the added components include the following components, the final concentration of each component in the staining solution is: hematoxylin 6g / L, aluminum potassium sulfate 40g / L and citric acid 3g / L.

[0038] The dyeing time of applying the dyeing liquid of this embodiment only needs 6 minutes, and the dyeing liquid can be continuously used for one and a half years. Proved by tissue section staining experiment (please see figure 1 and figure 2 ), the nuclei were clearly stained, and the structure of chromatin, nuclear membrane and nucleolus was clear.

Embodiment 2

[0040] In this embodiment, the mercury-free hematoxylin staining solution is composed of a mixed solution and additional components, wherein the mixed solution is composed of the following components in parts by volume: 1500 mL of distilled water, 100 mL of glycerol, and 60 mL of 2% periodic acid aqueous solution , the added components include the following components, the final concentration of each component in the staining solution is: hematoxylin 8g / L, aluminum potassium sulfate 60g / L and citric acid 6g / L.

[0041] The dyeing time of applying the dyeing solution of this embodiment only needs 5 minutes, and the dyeing solution can be continuously used for one and a half years. The tissue section staining experiment proved that the nuclei were clearly stained, and the structure of chromatin, nuclear membrane and nucleolus was clear.

Embodiment 3

[0043]In the present embodiment, the mercury-free hematoxylin staining solution is composed of a mixed solution and added components, wherein the mixed solution is composed of the following components in parts by volume: 1000 mL of distilled water, 80 mL of glycerol and 60 mL of 1% periodic acid aqueous solution, The added components include the following components, and the final concentration of each component in the staining solution is: hematoxylin 7g / L, aluminum potassium sulfate 50g / L, citric acid 5g / L and carbon nanoparticles, the carbon nanoparticles The particle size is 10-100nm, and the mass percentage of the carbon nano particles relative to the mixed solution is 0.005%.

[0044] The dyeing time of applying the dyeing liquid of this embodiment only needs 3 minutes, and the dyeing liquid can be continuously used for two years. The tissue section staining experiment proved that the nuclei were clearly stained, and the structure of chromatin, nuclear membrane and nucle...

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Abstract

The invention discloses a mercury-free hematoxylin dyeing solution, which consists of a mixed solution and added components, wherein the mixed solution consists of the following components by volume: 1000-1500 parts of distilled water, 70-100 parts of glycerin, and 40-60 parts of 1-2% periodic acid aqueous solution, the added components include the following components, and the final concentrations of each component in the dyeing solution are: hematoxylin 6-8 g/L, aluminum potassium sulfate 40 ~60g/L and citric acid 3~6g/L. The mercury-free hematoxylin dyeing solution of the invention has short dyeing time and good dyeing effect. The stained cells showed clear nuclear staining and clear chromatin, nuclear membrane and nucleolar structures.

Description

technical field [0001] The invention belongs to the technical field of pathological experiments, and relates to in vitro diagnostic reagents, in particular to a mercury-free hematoxylin staining solution. Background technique [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, as long as there is an operation, the pathology department of the hospital will make a histopathological diagnosis on the surgical tissue samples. [0003] Conventional paraffin-embedded HE staining is the most basic technique currently used to process tissue samples, it is the basis of pathological diagnostic methodology, and it is also the reference standard for evaluating the advantages and disadvantages of many new methods and technologies. The traditional method is to place the tissue sample in the tank of the treatment solution, pour or transfer in different treatment solutions in turn, and the sample is soaked in each reagent for different time to complet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 李明王德田
Owner 北京九州柏林生物科技有限公司
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