Fungus staining solution and method

Abstract

The invention relates to a fungus staining solution and method. The staining solution is divided into a solution A and a solution B, wherein the solution A is an aqueous solution of potassium hydroxide with concentration of 9wt%-15wt%, and the solution A also contains gentiobiose with concentration of 0.1wt%-0.3wt%; the solution B is a methylene blue solution of phenol, the concentration of methylene blue in the solution B is 0.1wt%, the concentration of phenol the in solution B is 2vol%-3vol%, and the solution B also contains L-lysine hydrochloride with concentration of 0.1wt%-0.3wt%. The fungus staining solution has the advantage of small dosage during use, also has good staining effect, can distinguish fine oil drops and fungi very efficiently in fungus detection, improves relevant inspection work efficiency of hospitals, and is suitable for popularization. Correspondingly, the invention provides the fungus staining method adopting the fungus staining solution and an application ofthe fungus staining solution in direct microscopy identification of fungi.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a fungal staining liquid and a method for staining fungi using the fungal staining liquid. Background technique [0002] In the laboratory of the hospital, laboratory staff often need to test substances such as leucorrhea, ear canal secretions, nail scales, and dander. During the inspection, it is necessary to distinguish the background substance from the fungus in order to detect the fungus. However, some specimens contain a small amount of lipid droplets. Because of the small amount of lipid droplets, very small oil droplets are prone to appear, and these very small oil droplets are not suitable to be distinguished from fungal spores. However, the existing staining technology is cumbersome to stain fungi and fungal spores when processing outpatient specimens, and cannot issue test results in a short time. Therefore, it becomes urgent to provide an efficient staining solution ...

AI Technical Summary

Problems solved by technology

However, some specimens contain a small amount of lipid droplets. Because of the small amount of lipid droplets, very small oil droplets are prone to appear, and these very small oil droplets are not suitable

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